人參皂苷Rb1治療兔耳增生性瘢痕相關(guān)機(jī)制的探索
發(fā)布時(shí)間：2018-08-03 11:54
【摘要】：目的:通過(guò)兔耳創(chuàng)建增生性疤痕模型,疤痕內(nèi)注射藥物人參皂苷Rb1,觀察療效及探索相關(guān)機(jī)理。方法:隨機(jī)取8只大耳型新西蘭白兔,每耳6處直徑1cm圓形傷口,創(chuàng)建增生性疤痕模型,待其上皮化后設(shè)空白組(N),地塞米松陽(yáng)性對(duì)照組(D),生理鹽水對(duì)照組(S),人參皂苷Rb1組(R)。其中地塞米松組及人參皂苷組均應(yīng)用生理鹽水配置濃度為1mg/ml,疤痕內(nèi)注射藥物,每次0.3ml,每三天1次,共三次。注射后第1、2、4、8周隨機(jī)收集2只兔子標(biāo)本(24例)行HE染色觀察瘢痕增生指數(shù)(SEI)、行Masson染色觀察膠原增生情況、免疫組化法檢測(cè)增值細(xì)胞核抗原(PCNA)表達(dá)、逆轉(zhuǎn)錄聚合酶鏈反應(yīng)檢測(cè)轉(zhuǎn)化生長(zhǎng)因子β(TGF-β)及Ⅰ型膠原表達(dá)。各組數(shù)據(jù)除以空白組數(shù)據(jù)進(jìn)行數(shù)據(jù)標(biāo)準(zhǔn)化。軟件使用SPSS19.0,計(jì)量資料以“x±s”表示,使用方差分析,若數(shù)據(jù)不符合正態(tài)性及方差齊性,改用非參數(shù)檢驗(yàn)。選α=0.05,當(dāng)P≤0.05,數(shù)據(jù)有統(tǒng)計(jì)學(xué)意義。結(jié)果:用藥后第1、2周三組間未見(jiàn)統(tǒng)計(jì)學(xué)差異,第4、8周,D及R組較S組瘢痕增生改善明顯,并隨時(shí)間的延長(zhǎng)療效增加,SHI降低(P0.05),膠原增生面積減少(P0.05),PCNA表達(dá)降低(P0.05),TGF-β1及Ⅰ型膠原m RNA表達(dá)降低(P0.05),但D組及R組間未見(jiàn)統(tǒng)計(jì)學(xué)差異。結(jié)論:局部注射人參皂苷Rb1能抑制活性細(xì)胞增殖,血管生成減少,抑制TGF-β1表達(dá),膠原合成減少,有效改善兔耳疤痕的增生,為使用人參皂苷Rb1防治疤痕形成提供理論基礎(chǔ)。
[Abstract]:Aim: to establish a hypertrophic scar model in rabbit ear and inject ginsenoside Rb1 into the scar to observe the curative effect and explore the related mechanism. Methods: eight New Zealand white rabbits with large ears were randomly selected, with 6 diameter 1cm round wounds in each ear, and then the hypertrophic scar model was created. After epithelization, a blank group of (N), dexamethasone positive control group, (D), normal saline control group, (S), ginsenoside Rb1 group (R). Was set up. The dexamethasone group and ginsenoside group were treated with saline (1 mg / ml). The drug was injected into the scar with 0.3 ml once every three days for three times. Two rabbits (24 cases) were randomly collected at 8 weeks after injection to observe the proliferation of collagen by Masson staining and the expression of proliferating cell nuclear antigen (PCNA) by immunohistochemical staining. The expression of transforming growth factor 尾 (TGF- 尾) and type I collagen were detected by reverse transcriptase polymerase chain reaction (RT PCR). Each group of data divided by blank group data for data standardization. The software uses SPSS 19.0, the measurement data is expressed as "x 鹵s", and the analysis of variance is used. If the data do not conform to normality and homogeneity of variance, the non-parametric test is used. When P 鈮，
本文編號(hào)：2161662
[Abstract]:Aim: to establish a hypertrophic scar model in rabbit ear and inject ginsenoside Rb1 into the scar to observe the curative effect and explore the related mechanism. Methods: eight New Zealand white rabbits with large ears were randomly selected, with 6 diameter 1cm round wounds in each ear, and then the hypertrophic scar model was created. After epithelization, a blank group of (N), dexamethasone positive control group, (D), normal saline control group, (S), ginsenoside Rb1 group (R). Was set up. The dexamethasone group and ginsenoside group were treated with saline (1 mg / ml). The drug was injected into the scar with 0.3 ml once every three days for three times. Two rabbits (24 cases) were randomly collected at 8 weeks after injection to observe the proliferation of collagen by Masson staining and the expression of proliferating cell nuclear antigen (PCNA) by immunohistochemical staining. The expression of transforming growth factor 尾 (TGF- 尾) and type I collagen were detected by reverse transcriptase polymerase chain reaction (RT PCR). Each group of data divided by blank group data for data standardization. The software uses SPSS 19.0, the measurement data is expressed as "x 鹵s", and the analysis of variance is used. If the data do not conform to normality and homogeneity of variance, the non-parametric test is used. When P 鈮，
本文編號(hào)：2161662
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