【摘要】：目的觀察微小RNA-200c(miR-200c)與酪氨酸激酶受體B(Trk B)腰椎間盤髓核組織中的表達變化,并探討其與腰椎間盤髓核退變程度的相關性。方法收集腰椎間盤髓核組織標本60例,用Pfirmlann分級對髓核退變程度進行分級,Ⅱ、Ⅲ、Ⅳ級各20例。采用細胞衰老β-半乳糖苷酶實驗檢測不同分級髓核組織中衰老髓核細胞百分比,RT-PCR技術檢測髓核組織中miR-200c、Trk B mRNA表達,Western blotting技術檢測髓核組織中Trk B蛋白表達,采用Pearson相關分析miR-200c、Trk B與衰老髓核細胞的關系。結果 PfirmlannⅡ、Ⅲ、Ⅳ級髓核組織中衰老髓核細胞百分比、miR-200c相對表達量、Trk B mRNA及蛋白相對表達量兩兩比較,P均0.05。miR-200c mRNA與Trk B mRNA相對表達量呈負相關(r=-0.71,P0.05),miR-200c mRNA相對表達量與衰老髓核細胞百分比呈正相關(r=0.75,P0.05),Trk B mRNA相對表達量與衰老髓核細胞百分比呈負相關(r=-0.70,P0.05)。結論腰椎間盤髓核退變程度越高,miR-200c表達越高,Trk B mRNA表達越低;miR-200c可能通過下調Trk B表達參與腰椎間盤髓核組織的退變過程。
[Abstract]:Objective to observe the expression of small RNA-200c (miR-200c) and tyrosine kinase receptor (B (Trk B) in nucleus pulposus of lumbar disc and to explore the relationship between miR-200c and degeneration of nucleus pulposus of lumbar intervertebral disc. Methods 60 specimens of nucleus pulposus of lumbar intervertebral disc were collected. The grade of degeneration of nucleus pulposus was graded by Pfirmlann classification, including 20 cases of grade 鈪，

本文編號：2157983