【摘要】：檢測(cè)MMP-2、MMP-3、MMP-9、TIMP-3及Ⅱ型膠原細(xì)胞外基質(zhì)因子在正常兔關(guān)節(jié)軟骨細(xì)胞和劃傷后不同時(shí)間點(diǎn)的表達(dá)變化。在無(wú)菌條件下獲取兔膝關(guān)節(jié)軟骨細(xì)胞,原代體外培養(yǎng)軟骨細(xì)胞,六孔板內(nèi)制備細(xì)胞損傷模型。用顯微鏡觀察正常細(xì)胞和損傷后1 d、3 d和7 d 3個(gè)時(shí)間點(diǎn)的軟骨細(xì)胞增殖情況。采用實(shí)時(shí)PCR檢測(cè)正常軟骨細(xì)胞和損傷后1 d、3 d和7 d 3個(gè)時(shí)間點(diǎn)的基質(zhì)金屬蛋白酶-2、3和9,基質(zhì)金屬蛋白酶抑制物-3及Ⅱ型膠原的m RNA的表達(dá)水平。成功分離軟骨,經(jīng)原代培養(yǎng)后成功建立損傷細(xì)胞模型。MMP-2損傷后第1天與正常組相比升高,第3天時(shí)下降,第7天明顯水平最高。與正常組相比,MMP-3在細(xì)胞損傷后第1天表達(dá)明顯下降,隨后第3天逐漸下降,第7天與第3天未見明顯變化。MMP-9劃傷后第1天比正常組升高,第3天上升至最高水平,第7天下降。TIMP-3基因在損傷后第1天與正常組相比明顯下降,隨后第3天稍有升高,第7天水平最低。Ⅱ型膠原劃傷后第1天與正常組相比明顯升高,隨后第3天呈下降趨勢(shì)接近于正常組,第7天又呈上升趨勢(shì)。MMP-2、MMP-3、MMP-9、TIMP-3因子和Ⅱ型膠原在細(xì)胞損傷后的不同時(shí)間點(diǎn)有不同的表達(dá)行為,這可為調(diào)節(jié)細(xì)胞外基質(zhì)基因治療關(guān)節(jié)軟骨損傷選擇合適的靶基因和時(shí)間窗提供實(shí)驗(yàn)依據(jù)。
[Abstract]:The expression of MMP-2, MMP-3, MMP-9, TIMP-3 and extracellular matrix factor (ECF) in normal rabbit articular chondrocytes and at different time points after scratch were detected. The chondrocytes of rabbit knee joint were obtained under aseptic condition. The primary cultured chondrocytes were cultured in vitro. The proliferation of normal cells and chondrocytes at 3 and 7 days after injury were observed by microscope. The expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-3 (MMP-2), matrix metalloproteinase-3 (MMP-2) and collagen type 鈪，

本文編號(hào)：2149730