【摘要】：【目的】檢測(cè)ADAMTS-7、ADAMTS-12在人體腰椎間盤髓核組織中的表達(dá)情況,探討ADAMTS-7、ADAMTS-12在人體腰椎間盤髓核組織退變中的作用,為研究人體腰椎間盤退變的機(jī)制提供一種新的研究方向�！痉椒ā繉�(shí)驗(yàn)組40例,為手術(shù)切除腰椎間盤突出癥患者退變的椎間盤組織,其中后外層纖維環(huán)破裂22例,后外層纖維環(huán)未破裂18例;對(duì)照組10例,為腰椎骨折患者正常的腰椎間盤組織;采用半定量RT-PCR和免疫組織化學(xué)技術(shù)分別從m RNA和蛋白質(zhì)水平檢測(cè)標(biāo)本中ADAMTS-7、ADAMTS-12的表達(dá)情況,進(jìn)行統(tǒng)計(jì)學(xué)分析。【結(jié)果】ADAMTS-7、ADAMTS-12蛋白質(zhì)表達(dá)含量在后外層纖維環(huán)破裂組和后外層纖維環(huán)未破裂組均明顯高于正常組,后外層纖維環(huán)破裂組又明顯高于后外層纖維環(huán)未破裂組(P0.01);ADAMTS-7、ADAMTS-12 m RNA表達(dá)量在后外層纖維環(huán)破裂組和后外層纖維環(huán)未破裂組均明顯高于正常組,后外層纖維環(huán)破裂組又明顯高于后外層纖維環(huán)未破裂組(P0.01)。【結(jié)論】ADAMTS-7、ADAMTS-12均有可能參與人體腰椎間盤組織退變的病理過(guò)程,有一定的臨床研究意義。且隨著人體腰椎間盤組織退變程度的增加,ADAMTS-7、ADAMTS-12的表達(dá)逐漸升高。
[Abstract]:[objective] to detect the expression of ADAMTS-7 and ADAMTS-12 in the nucleus pulposus of human lumbar disc, and to explore the role of ADAMTS-7 and ADAMTS-12 in the degeneration of the nucleus pulposus of human lumbar intervertebral disc. To provide a new research direction for studying the mechanism of degeneration of human lumbar intervertebral disc. [methods] Forty patients with lumbar disc herniation underwent surgical excision of degenerated intervertebral disc tissue, 22 of them had posterior outer fibrous ring rupture. There were 18 cases of unruptured posterior outer fibrous ring and 10 cases of control group, which were normal lumbar intervertebral disc tissue. The expression of ADAMTS-7 and ADAMTS-12 was detected by semi-quantitative RT-PCR and immunohistochemistry from m RNA and protein levels, respectively. [results] the protein expression of ADAMTS-7 and ADAMTS-12 was significantly higher in the group of the rupture of the posterior outer fiber ring and the group of the non-rupture of the posterior outer fiber ring than that of the normal group. The expression of ADAMTS-7 and ADAMTS-12 m RNA in the ruptured posterior outer fibrous ring group was significantly higher than that in the unruptured posterior outer fibrous ring group (P0.01), and the expression of ADAMTS-12 m RNA in the posterior outer fibrous ring rupture group and the posterior outer fibrous ring unruptured group was significantly higher than that in the normal group. ADAMTS-7 and ADAMTS-12 may be involved in the pathological process of degeneration of human lumbar intervertebral disc, which has some clinical significance. The expression of ADAMTS-12 increased gradually with the increase of degeneration degree of human lumbar intervertebral disc.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R681.53

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 盧永江;徐杰;;退變腰椎間盤髓核組織中ADAMTS-5蛋白表達(dá)的臨床意義[J];福建醫(yī)藥雜志;2010年03期

2 俞海明;李毅中;;炎癥在腰椎間盤退變、突出、吸收發(fā)病機(jī)制中的作用[J];國(guó)際骨科學(xué)雜志;2006年04期

3 崔春榮,劉文剛;腰椎間盤突出癥的退變機(jī)制與實(shí)驗(yàn)研究概況[J];河南中醫(yī)學(xué)院學(xué)報(bào);2003年03期

4 董振風(fēng);黃秀芳;董永東;李垂青;文明;;IL-8及TNF在突出椎間盤中的表達(dá)意義[J];濰坊醫(yī)學(xué)院學(xué)報(bào);2008年05期

5 張威林;王海強(qiáng);陳宇飛;劉志恒;張泳照;萬(wàn)中元;孫振;羅卓荊;;椎間盤退變的分子機(jī)制研究現(xiàn)狀[J];脊柱外科雜志;2012年04期

6 陳衛(wèi)明;徐鴿;金若敏;;腰椎間盤突出癥病因機(jī)制的研究進(jìn)展[J];時(shí)珍國(guó)醫(yī)國(guó)藥;2007年06期

7 常強(qiáng);馬迅;牛建鵬;王詠波;;ADAMTS-4在退變腰椎間盤髓核組織中的表達(dá)及其意義[J];山西醫(yī)科大學(xué)學(xué)報(bào);2008年05期

相關(guān)博士學(xué)位論文 前1條

1 田野;促炎因子誘導(dǎo)ADAMTS-4表達(dá)促進(jìn)椎間盤退變的分子機(jī)制研究[D];第二軍醫(yī)大學(xué);2013年

相關(guān)碩士學(xué)位論文 前1條

1 張林華;TNF-α、IL-1β在退變椎間盤組織中的表達(dá)及其意義[D];安徽醫(yī)科大學(xué);2010年

，

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