本文選題：外科腦損傷 + -脂氧合酶�。� 參考：《第二軍醫(yī)大學(xué)學(xué)報》2017年06期

【摘要】：目的研究大鼠外科腦損傷(SBI)模型腦組織中5-脂氧合酶(5-LOX)表達(dá)的空間分布、細(xì)胞定位和隨時間變化的規(guī)律,并探討其在SBI發(fā)病中的可能作用機制。方法將72只健康成年雄性SD大鼠隨機分為假手術(shù)(Sham)組及SBI術(shù)后1d(SBI-1d)組、3d(SBI-3d)組、7d(SBI-7d)組,每組18只。對SBI-1d、SBI-3d和SBI-7d組大鼠采用右側(cè)額葉切除法建立SBI模型,Sham組大鼠只移除相應(yīng)部位的顱骨,不傷及硬腦膜。采用干濕質(zhì)量法測量損傷同側(cè)、對側(cè)腦組織的含水量;Garcia評分和杠桿平衡評分評估各組大鼠的神經(jīng)行為學(xué)功能;免疫熒光化學(xué)染色確定5-LOX的空間分布及細(xì)胞定位;蛋白質(zhì)印跡法檢測5-LOX和核轉(zhuǎn)錄因子(NF)-κB的蛋白表達(dá);生物化學(xué)法測定損傷區(qū)周圍腦組織中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。結(jié)果 (1)與Sham組相比,SBI-1d和SBI-3d組大鼠損傷側(cè)腦組織含水量增加(P0.05);SBI-1d、SBI-3d和SBI-7d組大鼠出現(xiàn)神經(jīng)功能障礙(P0.01),SBI-7d組大鼠的Garcia評分較SBI-1d、SBI-3d組改善(P0.05)。(2)5-LOX主要分布在損傷區(qū)周圍的腦組織中,其中以神經(jīng)元表達(dá)為主,星形膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞也有表達(dá)。(3)與Sham組相比,SBI-1d和SBI-3d組大鼠損傷區(qū)周圍腦組織中5-LOX、NF-κB表達(dá)增加(P0.05),而SBI-7d組兩者表達(dá)低于SBI-1d和SBI-3d組(P0.05),其中5-LOX表達(dá)增加在SBI術(shù)后第1天最明顯。(4)與Sham組相比,SBI-1d、SBI-3d和SBI-7d組大鼠損傷區(qū)周圍腦組織中SOD活性降低(P0.05),SBI-1d和SBI-3d組MDA含量增加(P0.05)。結(jié)論 SBI后,5-LOX主要表達(dá)于損傷區(qū)周圍神經(jīng)元,星形膠質(zhì)細(xì)胞和小膠質(zhì)細(xì)胞也有表達(dá);且在SBI術(shù)后1d表達(dá)最多。5-LOX加重腦損傷可能與NF-κB表達(dá)增多以及氧化應(yīng)激增加有關(guān)。
[Abstract]:Objective to study the spatial distribution of 5- lipoxygenase (5-LOX) expression in the brain tissue of the rat model of surgical brain injury (SBI), the regularities of cell location and time change, and to explore the possible mechanism of its role in the pathogenesis of SBI. Methods 72 healthy adult male SD rats were randomly divided into sham operation (Sham) group and 1D (SBI-1d) group after SBI, 3D (SBI-3d) group, 7 D (SBI-7d) group, 18 rats in each group. SBI model was established by right frontal lobectomy in the rats of SBI-1d, SBI-3d and SBI-7d. The rats in group Sham only removed the skull of the corresponding parts and did not hurt the dura mater. The moisture content of the lateral brain tissue was measured by the dry wet mass method, and the Garcia score and the lever balance score were used to evaluate the neural behavior of each group. The spatial distribution and location of 5-LOX were determined by immunofluorescence staining, and the protein expression of 5-LOX and nuclear factor (NF) - kappa B was detected by Western blot; the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in the brain tissue around the damaged area were measured by biochemical method. Results (1) compared with the Sham group, the SBI-1d and SBI-3d groups were larger. The water content of the injured side of the rat brain was increased (P0.05); the rats in the SBI-1d, SBI-3d and SBI-7d groups had neural dysfunction (P0.01). The Garcia score of the rats in group SBI-7d was higher than that of SBI-1d and SBI-3d group (P0.05). (2) 5-LOX mainly distributed in the brain tissue around the injured area, mainly in neurons, astrocytes and microglia. (3) compared with the Sham group, the expression of 5-LOX and NF- kappa B in the brain tissue around the injured area of SBI-1d and SBI-3d rats increased (P0.05), but the expression of SBI-7d group was lower than that of SBI-1d and SBI-3d groups (P0.05), and the increase of 5-LOX expression was the most obvious after first days. (4) The activity decreased (P0.05), and the content of MDA in group SBI-1d and SBI-3d increased (P0.05). After SBI, 5-LOX was mainly expressed in the neurons around the injured area, astrocytes and microglia were also expressed, and the increase of 1D.5-LOX and the aggravation of brain injury after SBI operation may be related to the increase of NF- kappa B expression and the increase of oxidative stress.
【作者單位】： 暨南大學(xué)基礎(chǔ)醫(yī)學(xué)院生理學(xué)系;中山大學(xué)附屬第三醫(yī)院肝臟外科;
【基金】：廣東省科技計劃項目(2012B060300020) 中央高校基礎(chǔ)科研業(yè)務(wù)費專項基金(101201221612427) 廣東省醫(yī)學(xué)科研基金(A2016010) 廣州市科技計劃項目(2014J4100128)~~
【分類號】：R-332;R651.15

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