本文選題：糖尿病 + 代謝手術(shù)�。� 參考：《山東醫(yī)藥》2017年30期

【摘要】：目的探討胃腸轉(zhuǎn)流術(shù)對(duì)鏈脲佐菌素(STZ)所致胰島損傷大鼠的影響并探討其機(jī)制。方法選擇SD大鼠30只,隨機(jī)分為空白對(duì)照組、模型組、代謝手術(shù)組各10只。代謝手術(shù)組行改良Roux-en-Y胃腸轉(zhuǎn)流術(shù),空白對(duì)照組、模型組僅做單純開腹、關(guān)腹處理。手術(shù)7 d后,模型組、代謝手術(shù)組均腹腔注射STZ建立糖尿病模型,空白對(duì)照組腹腔注入等量生理鹽水。分別于造模前1天、造模后第7、14天時(shí)抽取各組大鼠空腹尾靜脈血,檢測(cè)血糖水平。于造模前1天、造模后2 h、造模后第7、14天時(shí)抽取空腹尾靜脈血,采用ELISA法檢測(cè)血漿C肽水平,以評(píng)價(jià)胰島損傷情況。于造模前1天、造模后24 h、造模后第7天時(shí)抽取空腹尾靜脈血,采用ELISA法檢測(cè)血漿TNF-α水平。于造模第2天時(shí)取胰腺組織,采用Western blotting法檢測(cè)凋亡相關(guān)蛋白B細(xì)胞淋巴瘤-2相關(guān)蛋白X(Bax)、B細(xì)胞淋巴瘤蛋白-2(Bcl2)、C/EBP環(huán)磷酸腺苷反應(yīng)元件結(jié)合轉(zhuǎn)錄因子同源蛋白(CHOP)表達(dá)。結(jié)果造模后第7、14天時(shí),模型組血糖高于造模前及同時(shí)間點(diǎn)空白對(duì)照組,代謝手術(shù)組血糖低于模型組(P均0.01)。造模后第7、14天時(shí),模型組血漿C肽水平均低于造模前及同時(shí)點(diǎn)空白對(duì)照組,代謝手術(shù)組血漿C肽水平均低于模型組(P均0.01)。造模后24 h及第7天時(shí),模型組血漿TNF-α水平均高于造模前及同時(shí)間點(diǎn)空白對(duì)照組,代謝手術(shù)組血漿TNF-α水平均低于模型組(P均0.01)。與代謝手術(shù)組相比,模型組Bcl2蛋白表達(dá)降低,CHOP、Bax蛋白表達(dá)增高(P均0.01)。結(jié)論胃腸轉(zhuǎn)流術(shù)可減輕STZ導(dǎo)致的大鼠胰腺組織損傷,其機(jī)制可能與減輕胰島細(xì)胞的炎癥反應(yīng)、抑制胰島細(xì)胞凋亡有關(guān)。
[Abstract]:Objective to investigate the effect of gastrointestinal bypass on islet injury induced by streptozotocin (STZ) in rats and its mechanism. Methods Thirty SD rats were randomly divided into control group, model group and metabolic surgery group. The metabolic operation group was treated with modified Roux-en-Y gastroenteropulmonary bypass, the blank control group and the model group were treated with simple laparotomy and abdominal closure. After 7 days of operation, the model group and the metabolic operation group were injected intraperitoneally with STZ to establish the diabetic model, and the blank control group were injected with the same amount of normal saline intraperitoneally. Fasting caudal venous blood samples were taken from rats on day 7 and 14 before modeling and blood glucose levels were detected. Fasting caudal venous blood was drawn on day 714 before and 2 hours after modeling. Plasma C-peptide levels were measured by Elisa to evaluate the injury of pancreatic islets. The plasma TNF- 偽 levels were measured by Elisa on the first day before and 24 hours after modeling, and on the 7th day after modeling, the fasting caudal venous blood was collected and the plasma levels of TNF- 偽 were measured by Elisa. On the 2nd day of model, pancreatic tissues were taken and the expression of apoptosis-related protein B cell lymphoma protein X (Bax) and B cell lymphoma protein 2 (BCL 2) C / EBP cyclic adenosine monophosphate receptor binding transcription factor (chop) was detected by Western blotting method. Results the blood glucose in the model group was higher than that in the blank control group before model making and at the same time point at the 14th day after modeling, and the blood glucose in the metabolic operation group was lower than that in the model group (P 0.01). The plasma C-peptide levels in the model group were lower than those in the model group and the blank control group at the same time, and the plasma C-peptide levels in the metabolic surgery group were lower than those in the model group (P0.01). The plasma TNF- 偽 levels in the model group were higher than those in the model group and the blank control group at the same time, and the plasma TNF- 偽 levels in the metabolic surgery group were lower than those in the model group (P0.01). Compared with the metabolic surgery group, the expression of Bcl2 protein in the model group was lower than that in the control group (P 0.01). Conclusion Gastrointestinal bypass can attenuate the pancreatic tissue injury induced by STZ in rats, and its mechanism may be related to the reduction of inflammatory reaction of islet cells and the inhibition of apoptosis of islet cells.
【作者單位】： 華北理工大學(xué)附屬醫(yī)院;
【基金】：河北省醫(yī)學(xué)科學(xué)研究重點(diǎn)課題計(jì)劃項(xiàng)目(20150085) 河北聯(lián)合大學(xué)科學(xué)研究基金項(xiàng)目任務(wù)(Z201427)
【分類號(hào)】：R587.1;R656

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 胥平湘;鄭陽;楊光華;邱振方;張國(guó)志;王長(zhǎng)友;;改良式十二指腸空腸轉(zhuǎn)流術(shù)動(dòng)物模型的建立與評(píng)價(jià)[J];中國(guó)現(xiàn)代普通外科進(jìn)展;2015年10期

相關(guān)碩士學(xué)位論文 前1條

1 鄭陽;胃轉(zhuǎn)流術(shù)聯(lián)合胰島移植治療糖尿病大鼠的實(shí)驗(yàn)研究[D];天津醫(yī)科大學(xué);2013年

【共引文獻(xiàn)】

相關(guān)期刊論文 前1條

1 張琪;鄭陽;楊光華;單雨靜;趙永魁;張博;陳建立;張國(guó)志;王長(zhǎng)友;;胃腸轉(zhuǎn)流術(shù)對(duì)鏈脲佐菌素所致大鼠胰腺損傷的影響[J];山東醫(yī)藥;2017年30期

相關(guān)碩士學(xué)位論文 前1條

1 趙方曉;振腹法對(duì)T2DM大鼠FBG、CRH、皮質(zhì)酮及腎上腺指數(shù)影響的研究[D];北京中醫(yī)藥大學(xué);2016年

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉斌;王瑜;王烈;黃盛;王暢;;正確認(rèn)識(shí)胃旁路術(shù)后并發(fā)癥[J];中國(guó)現(xiàn)代普通外科進(jìn)展;2010年10期

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4 劉洋;消化道轉(zhuǎn)流和非轉(zhuǎn)流重建術(shù)對(duì)2型糖尿病影響的回顧性分析（附57例臨床報(bào)告）[D];大連醫(yī)科大學(xué);2010年

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7 陳志雄;胃轉(zhuǎn)流術(shù)對(duì)2型糖尿病大鼠肝臟和胰腺Fox01表達(dá)影響的研究[D];重慶醫(yī)科大學(xué);2013年

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