本文選題：頸性眩暈 + 去甲腎上腺素��； 參考：《濟(jì)南大學(xué)》2017年碩士論文

【摘要】：目的:探討C2、C3脊神經(jīng)節(jié)與同側(cè)頸上交感神經(jīng)節(jié)之間的神經(jīng)纖維聯(lián)系及強(qiáng)度。方法:隨機(jī)選取新西蘭大白兔120只。隨機(jī)分為12組。(1)C2脊神經(jīng)節(jié)對照組定義為Y1組;C2脊神經(jīng)實(shí)驗(yàn)組定義為Y2組;C3脊神經(jīng)對照組為Y3組;C3脊神經(jīng)實(shí)驗(yàn)組為Y4組;頸上交感神經(jīng)節(jié)對照組定義為Y5組,頸上交感神經(jīng)節(jié)實(shí)驗(yàn)組定義為Y6組,分別給予Y2、Y4、Y6組的C2脊神經(jīng)、C3脊神經(jīng)、頸上交感神經(jīng)節(jié)注射熒光金試劑,給予Y1、Y3、Y5組的C2脊神經(jīng)、C3脊神經(jīng)、頸上交感神經(jīng)節(jié)注射生理鹽水,再繼續(xù)飼養(yǎng)4天后,取出Y1、Y2、Y3、Y4組的同側(cè)頸上交感神經(jīng)節(jié),分別取出Y5、Y6組同側(cè)C2、C3脊神經(jīng)節(jié)(定以為Y5-C2、Y5-C3、Y6-C2、Y6-C3組),分別做冰凍切片,然后在熒光顯微鏡下觀察、計(jì)數(shù)。(2)只解剖頸上交感神經(jīng)節(jié),取同側(cè)C2神經(jīng)節(jié)組定義為M1,取同側(cè)C3神經(jīng)節(jié)組定義為M3;電刺激頸上交感神經(jīng)節(jié),取同側(cè)C2神經(jīng)節(jié)組定義為M2組,取同側(cè)C3神經(jīng)節(jié)組定義為M4;只解剖C2神經(jīng)節(jié),取同側(cè)頸上交感神經(jīng)節(jié)定義為M5;電刺激C2神經(jīng)節(jié),取同側(cè)頸上交感神經(jīng)節(jié)定義為M6;只解剖C3神經(jīng)節(jié),取同側(cè)頸上交感神經(jīng)節(jié)定義為M7;電刺激C3神經(jīng)節(jié),取同側(cè)頸上交感神經(jīng)節(jié)定義為M8;分別行冰凍切片,然后使用免疫組織法檢測神經(jīng)節(jié)內(nèi)去甲腎上腺素、神經(jīng)肽Y的含量。結(jié)果:(1)Y1、Y3、Y5組的均未發(fā)現(xiàn)熒光金陽性細(xì)胞,Y2、Y4、Y6組均發(fā)現(xiàn)熒光金陽性細(xì)胞;Y2組與Y4組相比較,Y2組的熒光金陽性細(xì)胞數(shù)多于Y4組,差異有統(tǒng)計(jì)學(xué)意義(p0.05);Y6-C2組與Y6-C3組相比較,Y6-C2組的熒光金陽性細(xì)胞數(shù)多于Y6-C3組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。(2)去甲腎上腺素檢測:M2組與M1的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M4組與M3的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M6組與M5的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M8組與M7的平均光密度對比,平均光密度較小,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M6組與M8的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。(3)神經(jīng)肽Y檢測:M2組與M1的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M4組與M3的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M6組與M5的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M8組與M7的平均光密度對比,平均光密度較小,差異有統(tǒng)計(jì)學(xué)意義(p0.05);M6組與M8的平均光密度對比,平均光密度較大,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。結(jié)論:1.C2、C3脊神經(jīng)節(jié)與同側(cè)頸上交感神經(jīng)節(jié)存在神經(jīng)纖維聯(lián)系;2.C2脊神經(jīng)節(jié)與同側(cè)頸上交感神經(jīng)節(jié)的聯(lián)系強(qiáng)度大于C3脊神經(jīng)與同側(cè)頸上交感神經(jīng)的聯(lián)系強(qiáng)度。
[Abstract]:Objective: to study the nerve fiber connection and intensity between C _ 2 C _ 3 spinal ganglion and ipsilateral superior cervical sympathetic ganglion. Methods: 120 New Zealand white rabbits were randomly selected. They were randomly divided into 12 groups: group Y1, group Y2, group C 3, group Y3, group C 3, group Y4, and group Y5, the control group of superior cervical sympathetic ganglion, group Y5. The experimental group of superior cervical sympathetic ganglion was defined as Y6 group, which was treated with C2-spinal nerve C3-spinal nerve, supercervical sympathetic ganglion with fluorescent gold reagent, C2-spinal nerve group with C3 spinal nerve and superior cervical sympathetic ganglion with saline. After further feeding for 4 days, the ipsilateral superior cervical sympathetic ganglion (SCG) was removed from Y1OY2Y2OY3OY4 group, and the ipsilateral C2C3 spinal ganglion (Y5-C2C3Y6-C3-Y6-C3 group) was removed respectively, and the frozen sections were made and observed under fluorescence microscope. The ipsilateral C2 ganglion group was defined as M1, the ipsilateral C3 ganglion group was M3, and the ipsilateral C2 ganglion group was defined as M2 group by electrical stimulation of the superior cervical sympathetic ganglion. The ipsilateral C3 ganglion group was defined as M4; only the C2 ganglion was dissected, the ipsilateral superior cervical sympathetic ganglion was defined as M5; the ipsilateral superior cervical sympathetic ganglion was defined as M6 by electrical stimulation of C2 ganglion; only C3 ganglion was dissected. The ipsilateral superior cervical sympathetic ganglion was defined as M7, and the ipsilateral superior cervical sympathetic ganglion was defined as M8 by electrical stimulation of C3 ganglia. The contents of norepinephrine and neuropeptide Y in ganglion were determined by immunomechanical method. Results the number of fluorescent gold positive cells in Y2 group was higher than that in Y4 group, and the number of FG positive cells in Y2 group was higher than that in Y4 group, and the number of FG positive cells in Y2 group was higher than that in Y4 group, and the number of FG positive cells in Y2 group was higher than that in Y4 group, and the number of FG positive cells in Y2 group was higher than that in Y4 group. The number of fluorescent gold positive cells in Y6-C2 group was higher than that in Y6-C3 group, and the difference was statistically significant (P < 0.05). The average optical density of norepinephrine in M _ 2 group was higher than that in M _ 1 group, and the average optical density was higher in Y6-C2 group than in Y6-C3 group. The difference was statistically significant in the average optical density between group M4 and M3, the average optical density was larger than that in group M4, and there was significant difference between group M6 and group M5, and the average optical density of group M6 was higher than that of group M5, and the average optical density of group M6 was higher than that of group M5. The difference was statistically significant in the average optical density between the M8 group and the M7 group, the average optical density was smaller, the difference was statistically significant between the M8 group and the M8 group, and the average optical density was higher. There was significant difference in the average optical density between M 1 and M 2 group. The average optical density of M 2 group was higher than that of M 2 group, and the average optical density of M 3 group was higher than that of P 0.05 M4 group, and the average optical density of M 3 group was higher than that of M 3 group. The difference was statistically significant in the average optical density between the M6 group and the M5 group, the average optical density was larger, the difference was statistically significant between the M8 group and the M7 group, and the average optical density was smaller. The average optical density of M6 group was higher than that of M8 group, and the difference was significant (p 0.05). Conclusion 1. The connection between C _ 2 C _ 3 spinal ganglion and ipsilateral superior cervical sympathetic ganglion is stronger than that between C _ 3 spinal nerve and ipsilateral superior cervical sympathetic ganglia. (2) the connection between C _ 2 spinal ganglion and ipsilateral superior cervical sympathetic ganglion is stronger than that between C _ 3 spinal nerve and ipsilateral superior cervical sympathetic nerve.
【學(xué)位授予單位】：濟(jì)南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R681.55

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