本文選題：激素性股骨頭壞死 + 膠質(zhì)細(xì)胞源性神經(jīng)營(yíng)養(yǎng)因子��； 參考：《中國(guó)骨質(zhì)疏松雜志》2017年06期

【摘要】：目的探究膠質(zhì)細(xì)胞源性神經(jīng)營(yíng)養(yǎng)因子(glial cell line-derived neurotrophic factor,GDNF)對(duì)早期激素性股骨頭壞死(steroid-induced avascular necrosis of the femoral head,SANFH)的保護(hù)作用。方法采用改良技術(shù)建立早期激素性股骨頭壞死兔模型,隨機(jī)分成實(shí)驗(yàn)組(n=12)和對(duì)照組(n=12)。實(shí)驗(yàn)組給予髖周臀大肌注射GDNF(8.0μg/只),對(duì)照組相同部位注射等體積生理鹽水,干預(yù)1周。分別于第5、10、15、20天進(jìn)行股骨頭多排螺旋CT、顯微CT及蘇木素-伊紅(HE)染色檢測(cè)。結(jié)果影像學(xué)觀察示,GDNF注射后第15天開(kāi)始,實(shí)驗(yàn)組股骨頭骨小梁逐漸豐富、密集,間距變窄,骨皮質(zhì)厚度及密度增加;對(duì)照組股骨頭骨小梁分布稀疏,間距增寬,部分?jǐn)嗔?骨質(zhì)密度減低。第15、20天,實(shí)驗(yàn)組與對(duì)照組股骨頭骨礦物質(zhì)密度、組織骨礦物質(zhì)密度及骨體積分?jǐn)?shù),比較差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。組織學(xué)觀察示,實(shí)驗(yàn)組股骨頭內(nèi)骨小梁粗大,骨細(xì)胞分布廣泛,細(xì)胞核著色深,脂肪細(xì)胞分布較少;對(duì)照組骨小梁分布稀疏,形態(tài)不完整,部分?jǐn)嗔?骨細(xì)胞空陷窩和脂肪細(xì)胞增多,骨細(xì)胞和造血細(xì)胞減少。第15、20天,實(shí)驗(yàn)組與對(duì)照組股骨頭骨細(xì)胞空陷窩率分別為:13.3±3.2%、10.6±2.4%和71.0±7.5%、78.6±5.3%,兩組比較,實(shí)驗(yàn)組骨細(xì)胞空陷窩率顯著減低(P0.05)。結(jié)論 GDNF可通過(guò)抑制骨細(xì)胞壞死,保持骨小梁的完整性。
[Abstract]:Objective to investigate the protective effect of glial cell line-derived neurotrophic factor (GDF) on steroid-induced avascular necrosis of the femoral head (SANFH) in early steroid-induced femoral head necrosis. Methods the model of early steroid-induced femoral head necrosis in rabbits was established by modified technique and randomly divided into two groups: the experimental group and the control group. The experimental group was injected with GDNF 8.0 渭 g / rat, and the control group was injected with the same volume of normal saline for 1 week. Multislice spiral CT, microCT and hematoxylin-eosin HEH staining were performed on 15 ~ 20 days of 5 ~ (th) ~ 10 ~ (th) ~ (th) day of femoral head. Results from the 15th day after GDNF injection, the trabeculae of femoral head in the experimental group became richer, denser and narrower, the thickness and density of the bone cortex increased, and the trabeculae of the femoral head in the control group were distributed sparsely, the spacing was widened, and some fractures were observed. Bone density decreased. The bone mineral density, tissue bone mineral density and bone volume fraction of the femoral head in the experimental group and the control group were significantly higher than those in the control group (P 0.05). Histological observation showed that the trabeculae in the femoral head in the experimental group were thick, the bone cells were widely distributed, the nuclei were deeply stained, and the adipocytes were less, while the bone trabeculae in the control group were sparsely distributed, incomplete in shape and partially broken. The number of empty fossa and adipocytes increased, and the number of bone cells and hematopoietic cells decreased. On the 15th and 20th day, the empty recess rates of bone cells in the experimental group and the control group were respectively 10. 6 鹵2. 4% and 71. 0 鹵7. 5%, respectively. Compared with the control group, the rate of empty bone cells in the experimental group was significantly lower than that in the control group (10. 06 鹵2. 4% and 71. 0 鹵7. 5%). Conclusion GDNF can maintain the integrity of trabecular bone by inhibiting osteonecrosis.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院放射科;成都醫(yī)學(xué)院第一附屬醫(yī)院放射科;
【基金】：國(guó)家臨床重點(diǎn)專科建設(shè)項(xiàng)目([2013]544) 四川省教育廳基金資助項(xiàng)目(15ZB0243)
【分類號(hào)】：R681.8

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 吳潔,邱勇,孫燕芳,陳新;青少年特發(fā)性脊柱側(cè)凸患者骨密度變化的分析[J];中國(guó)脊柱脊髓雜志;2004年10期

2 梅鐵成;高玉紅;梅菊紅;蘇恩亮;張進(jìn)學(xué);;骨折病人與正常人骨密度的對(duì)比分析[J];中國(guó)骨質(zhì)疏松雜志;2005年02期

3 劉英杰;高順紅;白俊清;;外側(cè)半月板切除術(shù)對(duì)成人膝關(guān)節(jié)周圍骨密度的影響[J];中國(guó)骨質(zhì)疏松雜志;2006年03期

4 陳礎(chǔ)，朱建鋒，李惠萍;偏癱患者骨密度掃描35例報(bào)告[J];福建醫(yī)藥雜志;1994年04期

5 邵志宏，，李前鐸，孫雅琴;游泳對(duì)絕經(jīng)后婦女骨密度的影響[J];河南醫(yī)藥信息;1994年05期

6 徐明 ,楊恒;骨折危險(xiǎn)度的預(yù)測(cè):骨密度[J];國(guó)外醫(yī)學(xué)(臨床放射學(xué)分冊(cè));1997年06期

7 李巧羽;蘇濤;蔡小玲;;老年婦女骨折與骨密度關(guān)系的探討[J];實(shí)用預(yù)防醫(yī)學(xué);2010年03期

8 秦林林,陳金標(biāo),馬海波,葛崇華,張衛(wèi),肖艷霞;中國(guó)北方人骨密度及影響因素的多元回歸分析[J];中日友好醫(yī)院學(xué)報(bào);1997年03期

9 孫旭;邱勇;;青少年特發(fā)性脊柱側(cè)凸患者骨密度的研究進(jìn)展[J];中國(guó)脊柱脊髓雜志;2007年04期

10 楊定焯，楊小明;常用部位骨密度、骨紋、骨周的正常范圍的建議[J];職業(yè)衛(wèi)生與病傷;1995年01期

相關(guān)會(huì)議論文 前10條

1 張冬梅;RuthCox;TainaTaube;LindaReaney;JohnKanis;EugeneMcCloskey;;半自動(dòng)的方法測(cè)定掌骨皮質(zhì)指數(shù)和骨密度的臨床評(píng)價(jià)[A];全國(guó)老年骨質(zhì)疏松專題學(xué)術(shù)研討會(huì)論文匯編[C];2000年

2 黃任;謝華麗;;服用三髓粉1～5年對(duì)老年人骨密度、臨床癥狀及骨折的影響[A];中華醫(yī)學(xué)會(huì)第七次全國(guó)骨質(zhì)疏松和骨礦鹽疾病學(xué)術(shù)會(huì)議論文匯編[C];2013年

3 王玲;汪偉;馬毅民;程曉光;徐黎;;中國(guó)女性腹內(nèi)脂肪和皮下脂肪與骨密度的相關(guān)分析研究[A];中華醫(yī)學(xué)會(huì)第五次中青年骨質(zhì)疏松和骨礦鹽疾病學(xué)術(shù)會(huì)議論文集[C];2013年

4 杜峻華;林向進(jìn);;老年人股骨頸抗骨折能力的檢測(cè)與分析[A];2004年浙江省骨科學(xué)術(shù)會(huì)議論文匯編[C];2004年

5 汪偉;李佳錄;孔令懿;錢(qián)占華;陳祥述;程曉光;;肌肉的QCT測(cè)量及其與骨密度的關(guān)系[A];中華醫(yī)學(xué)會(huì)第三次骨質(zhì)疏松和骨礦鹽疾病中青年學(xué)術(shù)會(huì)議論文匯編[C];2011年

6 黃強(qiáng);沈彬;楊靜;周宗科;康鵬德;裴福興;;全髖關(guān)節(jié)表面置換術(shù)與大頭金屬對(duì)金屬全髖關(guān)節(jié)置換術(shù)后假體周圍骨密度比較[A];第20屆中國(guó)康協(xié)肢殘康復(fù)學(xué)術(shù)年會(huì)論文選集[C];2011年

7 朱繼珩;楊蕊敏;;老年人骨折與骨密度及骨代謝生化測(cè)定探討[A];中華醫(yī)學(xué)會(huì)第三次全國(guó)骨質(zhì)疏松和骨礦鹽疾病學(xué)術(shù)會(huì)議暨骨質(zhì)疏松診斷技術(shù)繼續(xù)教育學(xué)習(xí)班論文匯編[C];2004年

8 遇呈祥;陳亮;晏錚劍;楚磊;粱凱路;陳富;柯珍勇;鄧忠良;;骨密度對(duì)骨質(zhì)疏松性骨折的判斷價(jià)值[A];中華醫(yī)學(xué)會(huì)第五次中青年骨質(zhì)疏松和骨礦鹽疾病學(xué)術(shù)會(huì)議論文集[C];2013年

9 趙寧;高長(zhǎng)平;;關(guān)注兒童骨健康[A];第九屆全國(guó)骨質(zhì)疏松年會(huì)暨第六屆全國(guó)鈣劑年會(huì)會(huì)議文集[C];2003年

10 羅冬梅;秦嶺;;骨力學(xué)強(qiáng)度與骨密度間的相關(guān)研究[A];2002年第9屆全國(guó)運(yùn)動(dòng)醫(yī)學(xué)學(xué)術(shù)會(huì)議論文摘要匯編[C];2002年

相關(guān)重要報(bào)紙文章 前2條

1 馮衛(wèi)東;髖部骨密度含多種疾病信息[N];科技日?qǐng)?bào);2008年

2 呂乃群;消除骨折的危險(xiǎn)因素[N];衛(wèi)生與生活報(bào);2007年

相關(guān)碩士學(xué)位論文 前2條

1 王佳丹;絕經(jīng)后女性促甲狀腺激素生理變異對(duì)骨密度和骨折風(fēng)險(xiǎn)的影響[D];貴州醫(yī)科大學(xué);2016年

2 黃碧;女性習(xí)慣性飲茶對(duì)骨密度、骨質(zhì)疏松、低骨量及骨折風(fēng)險(xiǎn)影響的初步研究[D];中南大學(xué);2009年

本文編號(hào)：2022179