發(fā)布時(shí)間：2018-05-29 05:24

  本文選題：高脂STZ + 卵巢切除術(shù)　； 參考：《華北理工大學(xué)》2016年碩士論文

【摘要】：目的研究甲狀旁腺激素(Parathyroid hormone PTH)對(duì)高脂STZ誘導(dǎo)并卵巢切除大鼠骨折愈合的影響。方法將健康的50只2月齡雌性SD大鼠進(jìn)行適應(yīng)(環(huán)境和溫度以及濕度)飼養(yǎng)7天后,隨機(jī)進(jìn)行對(duì)大鼠分為5組,進(jìn)行實(shí)驗(yàn)研究。骨折+假手術(shù)組(F+Sham組)10只,骨折+雙側(cè)卵巢切除組(F+OVX組)10只,骨折+假手術(shù)+高脂STZ誘導(dǎo)(F+Sham+STZ組)10只,骨折+高脂STZ誘導(dǎo)+雙側(cè)卵巢切除(F+STZ+OVX組)10只,骨折+雙側(cè)卵巢切除+高脂STZ誘導(dǎo)+甲狀旁腺激素(F+OVX+STZ+PTH組)10只,各組大鼠均在骨折造模立刻行X線攝像評(píng)估右側(cè)股骨骨折情況。F+Sham和F+OVX組在三周腹腔注射檸檬酸緩沖液1ml/kg,而其他三組在3w腹腔注射STZ 35mg/kg。F+OVX+STZ+PTH組的閉合骨折造模手術(shù)后給予皮下注射PTH劑量為50ug/kg,每周5次,F+STZ+OVX組的閉合骨折造模手術(shù)后皮下注射等量的生理鹽水,每周5次。給藥三周后,處死前各組大鼠行右側(cè)股骨X線攝像,對(duì)骨折斷端愈合進(jìn)行放射學(xué)評(píng)分。應(yīng)用Image J軟件分析骨痂面積。處死動(dòng)物后留取右側(cè)股骨行骨組織切片番紅-o-固綠染色,免疫組化分析骨折斷端VEGF表達(dá)情況。結(jié)果1 X線檢測(cè)結(jié)果顯示F+Sham組骨折斷端邊緣完全消失,骨膜反應(yīng)密度近似骨影,骨痂填滿缺損,與骨皮質(zhì)密度基本相同并相互連接,F+STZ和F+OVX及F+STZ+OVX組可見骨骼組織骨折端骨痂組織細(xì)胞形成,但骨折組織斷端無(wú)連接,F+OVX+STZ+PTH組可見骨骼組織骨折端骨痂形成及骨痂組織細(xì)胞數(shù)量增多,骨折組織斷端可見小梁骨橋接形成骨連接,骨連接密度高。與F+Sham組相比,其余組骨折X線評(píng)分顯著低(P0.05),F+STZ+OVX與F+OVX+STZ+PTH組相比骨折X線評(píng)分顯著低(P0.05)。2骨骼組織骨折愈合骨痂的面積評(píng)估顯示F+Sham與F+STZ、F+STZ+OVX相比骨痂面積值高(P0.05),F+OVX與F+STZ+OVX相比骨骼組織骨痂面積低(P0.05),F+STZ+OVX與F+OVX+STZ+PTH組相比骨痂面積值升高(P0.05)。3免疫組織化結(jié)果顯示F+Sham組骨骼組織股骨干骨折斷端可見少量的VEGF的陽(yáng)性表達(dá),與F+Sham組相比F+STZ骨折斷端骨組織細(xì)胞中VEGF的陽(yáng)性表達(dá)下降,與F+Sham組相比F+OVX組可見VEGF的陽(yáng)性表達(dá)也下降,與F+STZ組相比,F+STZ+OVX組可見VEGF的陽(yáng)性表達(dá)也下降,與F+STZ+OVX組相比,F+OVX+STZ+PTH組骨折斷端VEGF的陽(yáng)性表達(dá)升高。結(jié)論1高脂STZ誘導(dǎo)并卵巢切除大鼠導(dǎo)致閉合性骨折斷端延期愈合。2 PTH治療可以促進(jìn)高脂STZ誘導(dǎo)并卵巢切除大鼠閉合性骨折斷端愈合,這可能與其上調(diào)骨痂中VEGF的表達(dá)水平有關(guān)。
[Abstract]:Objective to study the effect of parathyroid hormone (PTH) on fracture healing induced by hyperlipidemic STZ in ovariectomized rats. Methods 50 healthy 2-month-old female SD rats were fed with adaptation (environment, temperature and humidity) for 7 days, and were randomly divided into 5 groups. There were 10 rats in the sham operation group, 10 in the bilateral ovariectomy group, 10 in the F OVX group, 10 in the high fat STZ induced F Sham STZ group, and 10 in the bilateral ovariectomized STZ OVX group induced by high fat STZ. 10 rats were treated with bilateral ovariectomized with hyperlipidemic STZ induced parathyroid hormone (PTH) F OVX STZ PTH group (n = 10). All rats in each group were immediately treated with X-ray imaging to evaluate the fracture of the right femur. F Sham and F OVX groups were intraperitoneally injected with citric acid buffer solution of 1 ml / kg for three weeks, while the other three groups received intraperitoneal injection of closed fracture in STZ 35mg/kg.F OVX STZ PTH group for 3 weeks. PTH was subcutaneously injected with 50ugr / kg after model operation, and the same amount of normal saline was injected subcutaneously after closed fracture modeling operation in F STZ OVX group 5 times a week. Five times a week. After three weeks of administration, the rats in each group were given right femur X-ray before death, and the fracture healing was scored radiologically. The callus area was analyzed by Image J software. After the animals were killed, the right femur was taken to be stained with Fenghong-o-solid green staining and the expression of VEGF was analyzed by immunohistochemistry. Results 1the results of X-ray examination showed that the edge of fracture end disappeared completely, the periosteal reaction density was similar to bone shadow, and callus was filled with defect in F Sham group. Bone callus cells formed at the end of bone fracture were observed in F STZ, F OVX and F STZ OVX groups with the same density of bone cortex. In F OVX STZ PTH group, the formation of bone callus and the number of callus cells were increased. Trabecular bone bridging was seen in the broken end of fracture tissue, and the density of bone connection was high. Compared with F Sham group, Fracture X-ray score of the other groups was significantly lower than that of the F OVX STZ PTH group, the X-ray score of the fracture was significantly lower than that of the F OVX STZ PTH group. The evaluation of the area of bone callus healing of bone tissue showed that F Sham and F STZ F STZ OVX had higher callus area than that of F Sham and F STZ F STZ OVX (P 0.05 / F OVX and F STZ). Compared with the F OVX STZ PTH group, the bone callus area of OVX group was lower than that of the bone tissue callus area. The immunohistochemical results showed that a small amount of VEGF positive expression was found in the fracture end of the femoral shaft fracture in the F Sham group, compared with the value of the bone callus area in the F OVX STZ PTH group. Compared with F Sham group, the positive expression of VEGF in bone tissue of F STZ fracture was decreased, the positive expression of VEGF in F OVX group was lower than that in F Sham group, and the positive expression of VEGF in F STZ OVX group was lower than that in F STZ group. The positive expression of VEGF in fracture end of F OVX STZ PTH group was higher than that in F STZ OVX group. Conclusion (1) delayed healing of closed fracture induced by hyperlipidemic STZ and ovariectomized rats could promote the healing of closed fracture in hyperlipidemic STZ and ovariectomized rats, which may be related to the up-regulation of VEGF expression in callus.
【學(xué)位授予單位】：華北理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：R58;R683

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳同度,張昌穎;素食大鼠的貧血現(xiàn)象[J];營(yíng)養(yǎng)學(xué)報(bào);1957年04期

2 陳偉強(qiáng);趙善廣;;自制注射用大鼠固定裝置[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);1992年04期

3 肖柳英,林培英,馮昭明,張丹;不同周齡的SD大鼠生理、生化及體重的正常值測(cè)定[J];中藥新藥與臨床藥理;1996年03期

4 李淑云;簡(jiǎn)易大鼠灌胃器的制作[J];錦州醫(yī)學(xué)院學(xué)報(bào);2001年04期

5 楊明智,陳積圣;一種大鼠抓取與固定的新工具介紹[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2001年03期

6 戴英,陸群;復(fù)方H_(505)對(duì)Wistar大鼠外周血的血液流變學(xué)指標(biāo)的影響[J];中國(guó)血液流變學(xué)雜志;2001年01期

7 韋應(yīng)波,孫喜慶,曹新生,姚永杰,馮岱雅,楊長(zhǎng)斌;+Gz暴露時(shí)間對(duì)大鼠記憶功能和行為的影響[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2003年01期

8 呂學(xué)軍,郭俊生,李敏,周利梅,張永娟;暈船大鼠體內(nèi)鐵含量的變化[J];中國(guó)職業(yè)醫(yī)學(xué);2003年04期

9 湯仁仙,王迎偉,王慧,周峰;201A中藥合劑對(duì)大鼠抗腎小球基底膜腎炎病變的影響[J];徐州醫(yī)學(xué)院學(xué)報(bào);2003年06期

10 孫同柱,付小兵,翁立新,梁雪梅,陳偉;介紹一種簡(jiǎn)易的大鼠保定方法[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2004年01期

相關(guān)會(huì)議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對(duì)大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國(guó)顳下頜關(guān)節(jié)病學(xué)及（牙合）學(xué)大會(huì)論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號(hào)：1949658