本文選題：高脂STZ + 卵巢切除術��； 參考：《華北理工大學》2016年碩士論文

【摘要】：目的研究甲狀旁腺激素(Parathyroid hormone PTH)對高脂STZ誘導并卵巢切除大鼠骨折愈合的影響。方法將健康的50只2月齡雌性SD大鼠進行適應(環(huán)境和溫度以及濕度)飼養(yǎng)7天后,隨機進行對大鼠分為5組,進行實驗研究。骨折+假手術組(F+Sham組)10只,骨折+雙側卵巢切除組(F+OVX組)10只,骨折+假手術+高脂STZ誘導(F+Sham+STZ組)10只,骨折+高脂STZ誘導+雙側卵巢切除(F+STZ+OVX組)10只,骨折+雙側卵巢切除+高脂STZ誘導+甲狀旁腺激素(F+OVX+STZ+PTH組)10只,各組大鼠均在骨折造模立刻行X線攝像評估右側股骨骨折情況。F+Sham和F+OVX組在三周腹腔注射檸檬酸緩沖液1ml/kg,而其他三組在3w腹腔注射STZ 35mg/kg。F+OVX+STZ+PTH組的閉合骨折造模手術后給予皮下注射PTH劑量為50ug/kg,每周5次,F+STZ+OVX組的閉合骨折造模手術后皮下注射等量的生理鹽水,每周5次。給藥三周后,處死前各組大鼠行右側股骨X線攝像,對骨折斷端愈合進行放射學評分。應用Image J軟件分析骨痂面積。處死動物后留取右側股骨行骨組織切片番紅-o-固綠染色,免疫組化分析骨折斷端VEGF表達情況。結果1 X線檢測結果顯示F+Sham組骨折斷端邊緣完全消失,骨膜反應密度近似骨影,骨痂填滿缺損,與骨皮質密度基本相同并相互連接,F+STZ和F+OVX及F+STZ+OVX組可見骨骼組織骨折端骨痂組織細胞形成,但骨折組織斷端無連接,F+OVX+STZ+PTH組可見骨骼組織骨折端骨痂形成及骨痂組織細胞數(shù)量增多,骨折組織斷端可見小梁骨橋接形成骨連接,骨連接密度高。與F+Sham組相比,其余組骨折X線評分顯著低(P0.05),F+STZ+OVX與F+OVX+STZ+PTH組相比骨折X線評分顯著低(P0.05)。2骨骼組織骨折愈合骨痂的面積評估顯示F+Sham與F+STZ、F+STZ+OVX相比骨痂面積值高(P0.05),F+OVX與F+STZ+OVX相比骨骼組織骨痂面積低(P0.05),F+STZ+OVX與F+OVX+STZ+PTH組相比骨痂面積值升高(P0.05)。3免疫組織化結果顯示F+Sham組骨骼組織股骨干骨折斷端可見少量的VEGF的陽性表達,與F+Sham組相比F+STZ骨折斷端骨組織細胞中VEGF的陽性表達下降,與F+Sham組相比F+OVX組可見VEGF的陽性表達也下降,與F+STZ組相比,F+STZ+OVX組可見VEGF的陽性表達也下降,與F+STZ+OVX組相比,F+OVX+STZ+PTH組骨折斷端VEGF的陽性表達升高。結論1高脂STZ誘導并卵巢切除大鼠導致閉合性骨折斷端延期愈合。2 PTH治療可以促進高脂STZ誘導并卵巢切除大鼠閉合性骨折斷端愈合,這可能與其上調骨痂中VEGF的表達水平有關。
[Abstract]:Objective to study the effect of parathyroid hormone (PTH) on fracture healing induced by hyperlipidemic STZ in ovariectomized rats. Methods 50 healthy 2-month-old female SD rats were fed with adaptation (environment, temperature and humidity) for 7 days, and were randomly divided into 5 groups. There were 10 rats in the sham operation group, 10 in the bilateral ovariectomy group, 10 in the F OVX group, 10 in the high fat STZ induced F Sham STZ group, and 10 in the bilateral ovariectomized STZ OVX group induced by high fat STZ. 10 rats were treated with bilateral ovariectomized with hyperlipidemic STZ induced parathyroid hormone (PTH) F OVX STZ PTH group (n = 10). All rats in each group were immediately treated with X-ray imaging to evaluate the fracture of the right femur. F Sham and F OVX groups were intraperitoneally injected with citric acid buffer solution of 1 ml / kg for three weeks, while the other three groups received intraperitoneal injection of closed fracture in STZ 35mg/kg.F OVX STZ PTH group for 3 weeks. PTH was subcutaneously injected with 50ugr / kg after model operation, and the same amount of normal saline was injected subcutaneously after closed fracture modeling operation in F STZ OVX group 5 times a week. Five times a week. After three weeks of administration, the rats in each group were given right femur X-ray before death, and the fracture healing was scored radiologically. The callus area was analyzed by Image J software. After the animals were killed, the right femur was taken to be stained with Fenghong-o-solid green staining and the expression of VEGF was analyzed by immunohistochemistry. Results 1the results of X-ray examination showed that the edge of fracture end disappeared completely, the periosteal reaction density was similar to bone shadow, and callus was filled with defect in F Sham group. Bone callus cells formed at the end of bone fracture were observed in F STZ, F OVX and F STZ OVX groups with the same density of bone cortex. In F OVX STZ PTH group, the formation of bone callus and the number of callus cells were increased. Trabecular bone bridging was seen in the broken end of fracture tissue, and the density of bone connection was high. Compared with F Sham group, Fracture X-ray score of the other groups was significantly lower than that of the F OVX STZ PTH group, the X-ray score of the fracture was significantly lower than that of the F OVX STZ PTH group. The evaluation of the area of bone callus healing of bone tissue showed that F Sham and F STZ F STZ OVX had higher callus area than that of F Sham and F STZ F STZ OVX (P 0.05 / F OVX and F STZ). Compared with the F OVX STZ PTH group, the bone callus area of OVX group was lower than that of the bone tissue callus area. The immunohistochemical results showed that a small amount of VEGF positive expression was found in the fracture end of the femoral shaft fracture in the F Sham group, compared with the value of the bone callus area in the F OVX STZ PTH group. Compared with F Sham group, the positive expression of VEGF in bone tissue of F STZ fracture was decreased, the positive expression of VEGF in F OVX group was lower than that in F Sham group, and the positive expression of VEGF in F STZ OVX group was lower than that in F STZ group. The positive expression of VEGF in fracture end of F OVX STZ PTH group was higher than that in F STZ OVX group. Conclusion (1) delayed healing of closed fracture induced by hyperlipidemic STZ and ovariectomized rats could promote the healing of closed fracture in hyperlipidemic STZ and ovariectomized rats, which may be related to the up-regulation of VEGF expression in callus.
【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R58;R683

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