發(fā)布時(shí)間：2018-05-28 10:57

  本文選題：miRNA- + SOX��； 參考：《中國病理生理雜志》2017年02期

【摘要】：目的:探討骨肉瘤組織中miRNA-363及SOX4的表達(dá)水平,以及miRNA-363對骨肉瘤細(xì)胞活力、細(xì)胞周期以及凋亡的影響及機(jī)制。方法:用real-time PCR法檢測63例骨肉瘤患者腫瘤組織與癌旁組織中miRNA-363及SOX4的表達(dá)水平及相關(guān)性。用脂質(zhì)體法將miRNA-363 mimics瞬時(shí)轉(zhuǎn)染入MG-63人骨肉瘤細(xì)胞,檢測細(xì)胞中miRNA-363及SOX4的表達(dá)水平;CCK-8實(shí)驗(yàn)檢測各組細(xì)胞的活力;流式細(xì)胞術(shù)檢測各組細(xì)胞的周期及凋亡率;檢測轉(zhuǎn)染SOX4 siRNA或質(zhì)粒后miRNA-363的表達(dá)水平。結(jié)果:骨肉瘤組織miRNA-363表達(dá)明顯低于瘤旁組織,SOX4的mRNA表達(dá)明顯高于瘤旁組織,miRNA-363與SOX4的表達(dá)水平呈明顯負(fù)相關(guān)。轉(zhuǎn)染miRNA-363 mimics后MG-63細(xì)胞miRNA-363的表達(dá)水平明顯上調(diào),SOX4 mRNA及蛋白的表達(dá)水平明顯下降;同時(shí),細(xì)胞活力明顯下降細(xì)胞周期阻滯在G_0/G_1期,凋亡率增高。改變SOX4表達(dá)后,miRNA-363的表達(dá)無明顯變化,升高SOX4表達(dá)能夠恢復(fù)miRNA-363抑制的細(xì)胞活力。結(jié)論:miRNA-363在骨肉瘤中呈低表達(dá)水平,過表達(dá)miRNA-363可能通過調(diào)控SOX4抑制骨肉瘤細(xì)胞的生長,并促進(jìn)細(xì)胞凋亡。
[Abstract]:Aim: to investigate the expression of miRNA-363 and SOX4 in osteosarcoma, and the effect of miRNA-363 on the viability, cell cycle and apoptosis of osteosarcoma cells. Methods: the expression of miRNA-363 and SOX4 in 63 cases of osteosarcoma were detected by real-time PCR method. MiRNA-363 mimics was transiently transfected into MG-63 human osteosarcoma cells by liposome method. The expression levels of miRNA-363 and SOX4 were detected by CCK-8 assay, and the cell cycle and apoptosis rate were detected by flow cytometry. The expression level of miRNA-363 was detected after transfection of SOX4 siRNA or plasmid. Results: the expression of miRNA-363 in osteosarcoma was significantly lower than that in the adjacent tissues of osteosarcoma. The expression of miRNA-363 and SOX4 was negatively correlated with the expression of SSOX4 in paraneoplastic tissues. After transfection of miRNA-363 mimics, the miRNA-363 expression level of MG-63 cells decreased significantly, and the cell viability decreased significantly, and the cell cycle arrest in G_0/G_1 phase, and the apoptosis rate increased. There was no significant change in the expression of miRNA-363 after the change of SOX4 expression, but the increased expression of SOX4 could restore the cell viability inhibited by miRNA-363. Conclusion the expression of miRNA-363 is low in osteosarcoma. Overexpression of miRNA-363 may inhibit the growth of osteosarcoma cells and promote apoptosis by regulating SOX4.
【作者單位】： 新鄉(xiāng)醫(yī)學(xué)院第一附屬醫(yī)院骨科;新鄉(xiāng)市中心醫(yī)院神經(jīng)內(nèi)科;
【基金】：吳階平醫(yī)學(xué)基金會臨床科研專項(xiàng)資助基金(No.320274516224)
【分類號】：R738.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 翟，

本文編號：1946440