本文選題：KPNA2 + 骨肉瘤��； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：背景:核轉(zhuǎn)運(yùn)蛋白α2是核轉(zhuǎn)運(yùn)蛋白的一種,在腫瘤的發(fā)生發(fā)展中起著重要的作用。研究表明KPNA2在多種腫瘤中表達(dá),然而目前并無有關(guān)KPNA2在骨肉瘤中的作用相關(guān)報道。目的:本實(shí)驗研究骨肉瘤細(xì)胞系中和正常組織中KPNA2的蛋白及m RNA的表達(dá)差異。并進(jìn)一步研究KPNA2是否影響骨肉瘤細(xì)胞系Saos-2的增值和凋亡。方法:免疫組化(IHC)用于檢測骨肉瘤組織和正常骨組織中KPNA2的蛋白的表達(dá)量;實(shí)時熒光定量PCR(q PCR)用于檢測四種骨肉瘤細(xì)胞系中KPNA2m RNA表達(dá)水平,小片段RNA干擾慢病毒技術(shù)用于敲減Saos-2中KPNA2的表達(dá);四唑鹽比色法(MTT)和高內(nèi)涵篩選(HCS)用于檢測細(xì)胞的增值和生長;流式細(xì)胞術(shù)用于檢測細(xì)胞周期和細(xì)胞凋亡。結(jié)果:結(jié)果顯示骨肉瘤組織中KPNA2高表達(dá),并且在四種骨肉瘤細(xì)胞系中KPNA2m RNA也高表達(dá)。在轉(zhuǎn)染慢病毒后,與對照組相比,實(shí)驗組中細(xì)胞增值率明顯下降,流式結(jié)果顯示實(shí)驗組中在S期和G2/M期細(xì)胞數(shù)目明顯增多,表明KPNA2可能干擾Saos-2的細(xì)胞周期和細(xì)胞的生長。細(xì)胞凋亡的結(jié)果表明KPNA2-sh RNA組的凋亡率為13.38±0.48%,明顯高于對照組(5.13±0.33%)。結(jié)論:該實(shí)驗表明,KPNA2在骨肉瘤中高表達(dá),干擾骨肉瘤細(xì)胞中KPNA2的表達(dá)可抑制Saos-2細(xì)胞的增值,促進(jìn)其凋亡。
[Abstract]:Background: nuclear transporter 偽 2 is a nuclear transporter, which plays an important role in tumorigenesis and development. Studies have shown that KPNA2 is expressed in a variety of tumors, but there are no reports on the role of KPNA2 in osteosarcoma. Objective: to study the expression of KPNA2 protein and m RNA in osteosarcoma cell line and normal tissue. Furthermore, the effect of KPNA2 on the proliferation and apoptosis of osteosarcoma cell line Saos-2 was studied. Methods: immunohistochemistry was used to detect the expression of KPNA2 protein in osteosarcoma and normal bone tissue, and real-time fluorescence quantitative PCR(q was used to detect the expression of KPNA2m RNA in four kinds of osteosarcoma cell lines. Small fragment RNA interference lentivirus technique was used to knock down the expression of KPNA2 in Saos-2; tetrazolium colorimetric assay (MTT) and high intension screening assay were used to detect cell proliferation and growth; flow cytometry was used to detect cell cycle and apoptosis. Results: high expression of KPNA2 was found in osteosarcoma tissues and high expression of KPNA2m RNA in four kinds of osteosarcoma cell lines. Compared with the control group, the proliferation rate of lentivirus in the experimental group was significantly decreased, and the number of cells in S phase and G 2 / M phase was significantly increased in the experimental group, indicating that KPNA2 might interfere with the cell cycle and cell growth of Saos-2. The apoptosis rate in KPNA2-sh RNA group was 13.38 鹵0.48, which was significantly higher than that in control group (5.13 鹵0.33). Conclusion: KPNA2 is highly expressed in osteosarcoma and interfering with the expression of KPNA2 in osteosarcoma cells can inhibit the proliferation of Saos-2 cells and promote its apoptosis.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R738.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 孫曉莉;Abdallah Dlykan;賈宇;魏園玉;劉帥;岳保紅;;核干細(xì)胞因子RNA干擾慢病毒載體的構(gòu)建及鑒定[J];中華臨床醫(yī)師雜志(電子版);2013年11期

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