本文選題：病理性瘢痕 + Hippo信號(hào)通路��； 參考：《揚(yáng)州大學(xué)》2017年碩士論文

【摘要】：研究背景:瘢痕是臨床上常見的疾病之一,可分為病理性瘢痕和生理性瘢痕兩類。瘢痕常形成于創(chuàng)傷后,適度的瘢痕形成是一種生理性和自衛(wèi)性的表現(xiàn),但瘢痕過度增生則屬于病理性的表現(xiàn)。病理性瘢痕一般可分為增生性瘢痕和瘢痕疙瘩,常常對(duì)患者的外觀形象以及瘢痕部位的活動(dòng)功能等產(chǎn)生不良影響,同時(shí)也會(huì)造成患者的心理負(fù)擔(dān),嚴(yán)重者可導(dǎo)致抑郁癥等疾病發(fā)生�，F(xiàn)代社會(huì)隨著經(jīng)濟(jì)的發(fā)展和人們生活水平的提高,患者對(duì)于生活質(zhì)量的要求以及美容方面的要求越來越高,因此對(duì)于病理性瘢痕的病因探索、診斷治療新技術(shù)等的需求也更為強(qiáng)烈。病理性瘢痕的兩大特征為成纖維細(xì)胞的過度增殖與瘢痕的類腫瘤性特征。目前研究表明成纖維細(xì)胞是創(chuàng)傷后創(chuàng)面愈合的主要修復(fù)細(xì)胞,在創(chuàng)面修復(fù)的過程中活化、增殖、合成膠原,其過度增殖是病理性瘢痕的一個(gè)主要特征。病理性瘢痕在病因、病理機(jī)制、臨床表現(xiàn)、診斷和治療等方面與腫瘤均具有相似的生物學(xué)特性,即病理性瘢痕的類腫瘤性特性。病理性瘢痕的類腫瘤性特性一方面能夠較好、較全面地概述病理性瘢痕的生物學(xué)特性,另一方面強(qiáng)調(diào)了病理性瘢痕防治的復(fù)雜性和艱巨性,因此對(duì)于病理性瘢痕發(fā)生機(jī)制研究可借鑒腫瘤學(xué)的研究思路和成果。2005年,有學(xué)者首次在果蠅中發(fā)現(xiàn)Hippo信號(hào)通路傳遞從細(xì)胞質(zhì)到細(xì)胞核的細(xì)胞信號(hào),該通路能夠調(diào)節(jié)下游基因的表達(dá),從而調(diào)節(jié)細(xì)胞的生長(zhǎng)、增殖和凋亡,參與調(diào)控器官大小和組織再生,在胚胎發(fā)育和組織器官形成等過程中具有重要作用。研究表明Hippo信號(hào)通路參與調(diào)解多種成纖維細(xì)胞的形態(tài)功能,同時(shí)也與腫瘤的發(fā)生、發(fā)展有直接的關(guān)系。綜上所述,我們猜測(cè)Hippo信號(hào)通路在病理性瘢痕形成過程中發(fā)揮一定的作用。目前已知YAP/TAZ的表達(dá)在Hippo信號(hào)通路中發(fā)揮關(guān)鍵作用,因此本研究擬通過實(shí)驗(yàn)闡述Hippo信號(hào)通路關(guān)鍵性作用分子YAP/TAZ在病理性瘢痕中表達(dá)情況,探索Hippo信號(hào)通路在病理性瘢痕發(fā)生機(jī)制中的作用。目的:研究人真皮成纖維細(xì)胞及人皮膚增生性瘢痕成纖維細(xì)胞中Hippo信號(hào)通路關(guān)鍵作用分子YAP/TAZ表達(dá)情況,并初步建立增生性瘢痕兔耳模型,檢測(cè)兔耳增生性瘢痕中YAP/TAZ表達(dá)情況。方法:(1)流式細(xì)胞術(shù)檢測(cè)人真皮成纖維細(xì)胞及人皮膚增生性瘢痕成纖維細(xì)胞凋亡情況,免疫印跡試驗(yàn)(Western Blot)、逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)(Reverse transcriotion-polymerase chain reaction,RT-PCR)、定量實(shí)時(shí)聚合酶鏈反應(yīng)(Quantitative real time polymerase chain reaction,Q-PCR)方法檢測(cè)人真皮成纖維細(xì)胞及人皮膚增生性瘢痕成纖維細(xì)胞中Hippo信號(hào)通路主要作用分子YAP/TAZ表達(dá)情況。(2)兔耳瘢痕模型建立:用環(huán)鉆在每只兔耳腹側(cè)面各做6個(gè)直徑6mm的圓形全層皮膚缺損創(chuàng)面,每個(gè)創(chuàng)面間隔2cm以上,去除軟骨膜,創(chuàng)傷后約21天時(shí)取瘢痕組織做蘇木素一伊紅(Hematoxylin and eosin,HE)染色檢測(cè)瘢痕形成情況,用Western Blot方法檢測(cè)YAP/TAZ蛋白表達(dá)情況。結(jié)果:(1)人皮膚增生性瘢痕成纖維細(xì)胞凋亡弱于人真皮成纖維細(xì)胞,YAP/TAZ表達(dá)人皮膚增生性瘢痕成纖維細(xì)胞高于人真皮成纖維細(xì)胞。(2)兔耳創(chuàng)傷后約21天左右形成增生性瘢痕,HE染色顯示膠原增生,成纖維細(xì)胞增生,瘢痕組織YAP/TAZ表達(dá)高于正常兔耳組織。結(jié)論:Hippo信號(hào)通路特別是其關(guān)鍵作用分子YAP/TAZ在病理性瘢痕發(fā)生過程中起到一定的作用。
[Abstract]:Research background: scar is one of the common diseases in clinical. It can be divided into two types: pathological scar and physiological scar. Scar is often formed after trauma. Moderate scar formation is a physiological and self-defense manifestation, but hypertrophic scar is a pathological manifestation. Pathological cicatrix can be divided into hypertrophic scar and kelp. In one's heart, it often has a bad effect on the appearance of the patient and the activity function of the scar. It can also cause the psychological burden of the patient, and the serious person can cause the disease of depression. In modern society, with the development of the economy and the improvement of people's living standard, the more the patients demand for the quality of life and the beauty. The higher it is, the need for the pathogeny exploration of pathological scar and the new technique of diagnosis and treatment are also stronger. The two characteristics of pathological scar are the hyperproliferation of fibroblasts and the tumor like characteristics of scar. Activation, proliferation, and collagen synthesis, and their excessive proliferation is a major feature of pathological scars. Pathological scars have similar biological characteristics with tumors in the etiology, pathological mechanism, clinical manifestations, diagnosis and treatment, that is, the tumor like characteristics of the cicatricial cicatrix. The tumor like characteristics of pathological scars can be compared on one hand. Well, a comprehensive overview of the biological characteristics of pathological cicatrix is outlined. On the other hand, the complexity and arduous nature of the prevention and control of pathological scars is emphasized. Therefore, for the study of the pathogenesis of pathological cicatrix, the study of oncology can be used for reference in.2005 years. Some scholars first found the Hippo signaling pathway from the cytoplasm to the nucleus in the Drosophila. Cell signaling, which regulates the expression of downstream genes, regulates cell growth, proliferation and apoptosis, participates in the regulation of organ size and tissue regeneration, and plays an important role in the process of embryonic development and tissue formation. The study shows that Hippo signaling pathway participates in the mediation of the morphological functions of multiple fibroblasts, as well as in the form of fibroblasts. There is a direct relationship with the development of the tumor. In summary, we have guessed that the Hippo signaling pathway plays a role in the formation of pathological scar. The expression of YAP/TAZ is known to play a key role in the Hippo signaling pathway. Therefore, this study intends to elaborate the key role of the Hippo signaling pathway, YAP/TAZ, in the disease. To explore the role of Hippo signaling pathway in the pathogenesis of pathological scar. Objective: To study the expression of the key role of Hippo signaling pathway in human dermal fibroblasts and human dermal hypertrophic scar fibroblasts, and to establish an initial model of hypertrophic scar rabbit ears to detect the proliferation of rabbit ears. YAP/TAZ expression in the scar. Methods: (1) flow cytometry was used to detect the apoptosis of human dermal fibroblasts and human dermal hypertrophic scar fibroblasts, Western Blot, reverse transcription polymerase chain reaction (Reverse transcriotion-polymerase chain reaction, RT-PCR), and quantitative real-time polymerase chain reaction (Quantitative R). Eal time polymerase chain reaction, Q-PCR) method to detect the main role of Hippo signaling pathway YAP/TAZ expression in human dermal fibroblasts and human dermal hypertrophic scar fibroblasts. (2) the rabbit ear scar model was established: 6 round full layer skin defect wounds with diameter 6mm in each rabbit ear were made by ring drill. The wound interval was above 2cm, and the cartilage membrane was removed. The scar tissue was stained by Hematoxylin and eosin (HE) to detect the scar formation when the scar tissue was stained about 21 days after the trauma. The expression of YAP/TAZ protein was detected by Western Blot. Results: (1) the apoptosis of human dermal proliferative scar fibroblasts was weaker than human dermal fibroblasts, YAP/TAZ The expression of human dermal hypertrophic scar fibroblasts was higher than that of human dermal fibroblasts. (2) hypertrophic scar formed about 21 days after trauma in rabbit ears. HE staining showed collagen proliferation, fibroblast proliferation, and YAP/TAZ expression in scar tissue was higher than normal rabbit ear tissue. Conclusion: the Hippo signal pathway, especially its key molecule YAP/TAZ, is in pathology It plays a certain role in the process of sexual scarring.

【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R622

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊雪松;付_g;王永儉;于立紅;劉靈;;低壓無針注射聯(lián)合硅膠膜治療瘢痕疙瘩療效觀察[J];中國(guó)美容醫(yī)學(xué);2015年11期

2 鄭錦標(biāo);;增生性瘢痕和瘢痕疙瘩的非手術(shù)治療[J];北方藥學(xué);2015年03期

3 皮超;王鴻健;梁瑜珩;蔣艷;陳曉莉;高鵑;;凹陷性痤瘡瘢痕的治療進(jìn)展[J];臨床皮膚科雜志;2014年12期

4 王芳;章杰;劉偉;李文芳;;2940nm餌像素激光對(duì)早期、成熟瘢痕干預(yù)效果的臨床觀察[J];實(shí)用中西醫(yī)結(jié)合臨床;2013年08期

5 王家亮;徐陽;李海濤;祝賀;敖俊紅;楊蓉婭;;2940nm點(diǎn)陣鉺激光對(duì)兔耳增生性瘢痕血管內(nèi)皮生長(zhǎng)因子的影響[J];實(shí)用皮膚病學(xué)雜志;2013年03期

6 許傳銘;萬福生;;哺乳動(dòng)物Hippo信號(hào)通路:腫瘤治療的新標(biāo)靶[J];遺傳;2012年03期

7 閆貴春;裴銀輝;;瘢痕發(fā)生機(jī)制研究進(jìn)展[J];中國(guó)美容醫(yī)學(xué);2010年08期

8 于蓉;宋燁;陳俊杰;劉曉雪;岑瑛;;一種兔耳中期增生性瘢痕動(dòng)物模型的建立[J];華西醫(yī)學(xué);2010年07期

9 朱桂英;徐斌;蔡景龍;;兔耳解剖特點(diǎn)與成功建立增生性瘢痕模型的相關(guān)性實(shí)驗(yàn)研究[J];中華整形外科雜志;2008年03期

10 宗憲磊;姜篤銀;蔡景龍;徐斌;;瘢痕疙瘩的腫瘤特性研究進(jìn)展[J];中國(guó)美容整形外科雜志;2007年05期

相關(guān)碩士學(xué)位論文 前2條

1 閆倫;咪喹莫特對(duì)兔耳瘢痕增生抑制作用的實(shí)驗(yàn)研究[D];桂林醫(yī)學(xué)院;2013年

2 張傳永;瘢痕神經(jīng)構(gòu)筑及與瘙癢關(guān)系的研究[D];安徽醫(yī)科大學(xué);2001年

，

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