本文選題：原發(fā)性膝骨性關(guān)節(jié)炎 + TGF-β1　； 參考：《石河子大學》2017年碩士論文

【摘要】：目的探索并分析轉(zhuǎn)化生長因子β(transforming growth factor-beta1,TGF-β1)與白細胞介素1β(interleukin 1beta,IL-1β)基因中各基因型及等位基因頻率與原發(fā)性膝骨性關(guān)節(jié)炎(primary knee osteoarthritis,PKOA)的相關(guān)性方法本研究利用現(xiàn)況調(diào)查的基本方法,對新疆地區(qū)漢族、維族中老年人群OA的患病情況進行流行病學調(diào)查,根據(jù)患者的癥狀及放射線改變的證據(jù)篩選出患有OA的患者及健康體檢人群分別列入實驗組對照組。采用聚合酶鏈式反應(yīng)(polymerase chain reactionrestriction fragment length polymorphism,PCR-RFLP)技術(shù),檢測所有被研究者的TGF-β1-509C/T位點、-1348C/T位點以及IL-1β-511C/T位點的基因型,并對檢測出的單核苷酸多態(tài)性進行統(tǒng)計學分析,并將擴增結(jié)果送入北京六合華大基因科技股份有限公司進行基因測序,得出TGF-β1、IL-1β的基因多態(tài)性與0A的關(guān)系。結(jié)果1.在對照組及OA組TGF-β1-509C/T、-1348C/T位點以及IL-1β-511C/T位點均檢測出T、C兩種等位基因,其有三種組合基因型:TT型、CT型、CC型。2.轉(zhuǎn)化生長因子β1-1348C/T位點中漢族關(guān)節(jié)炎組CC、CT、TT的基因型頻率分別為19、74、36,對照組中CC、CT、TT的基因型頻率分別為73、134、37,并進行卡方檢驗,漢族關(guān)節(jié)炎與漢族對照組之間的χ2=14.986、檢驗統(tǒng)計量P=0.001;關(guān)節(jié)炎組等位基因C、T分別為112(0.43)、146(0.57),對照組等位基因分別為280(0.57)、208(0.43),OR為0.57,95%CI為(0.420~0.773)。3.白細胞介素1β-511C/T位點中維族關(guān)節(jié)炎組CC、CT、TT的基因型頻率分別為15、27、30,對照組中CC、CT、TT的基因型頻率分別為36、123、63,并進行卡方檢驗,漢族關(guān)節(jié)炎與漢族對照組之間的χ2=7.119、檢驗統(tǒng)計量P=0.028;維族關(guān)節(jié)炎組等位基因C、T分別為57(0.40)、87(0.60),維族對照組等位基因分別為195(0.44)、249(0.56),OR為0.837,95%CI為(0.570~1.227)。結(jié)論IL-1β-511C/T位點的TT基因型與維族關(guān)節(jié)炎的發(fā)生具有較高的相關(guān)性;TGF-β1-1348C/T位點的TT基因型與漢族關(guān)節(jié)炎的發(fā)生具有較高的相關(guān)性。
[Abstract]:Objective to explore and analyze the relationship between the frequencies of genotype and allele of transforming growth factor-beta1 (TGF- 尾 1) gene and interleukin-1 尾 interleukin 1beta1 尾 gene and primary knee osteoarthritis (PKOAA) in patients with primary knee osteoarthritis. The prevalence of OA in the Han and Uygur populations in Xinjiang was investigated. According to the symptoms of the patients and the evidence of radiographic changes, the patients with OA and the healthy people were selected as the control group. Polymerase chain reactionrestriction fragment length polymorphismPCR (PCR-RFLP) technique was used to detect the genotypes of TGF- 尾 1-509C/T locus and IL-1 尾 -511C / T locus in all the subjects, and the single nucleotide polymorphisms were analyzed statistically. The results of amplification were sent to Beijing Liuhe Huada Gene Technology Co., Ltd for gene sequencing. The relationship between gene polymorphism of TGF- 尾 1 and IL-1 尾 and 0A was obtained. Result 1. Two alleles of TGF- 尾 1-509C / TF- 1348C / T and IL-1 尾 -511C / T were detected in the control group and OA group. There were three genotypes of TGF- 尾 1-509C / T / T and IL-1 尾 -511C / T, and there were three genotypes of TGF- 尾 1-509C / TGF-1348C / T and IL-1 尾 -511C / T, respectively. The genotype frequencies of CTTTT in the Han nationality arthritis group were 1974f36 in the transforming growth factor 尾 1-1348C/T locus, and 73134r-37 in the control group, respectively, and chi-square test was performed. There was significant difference between the Han nationality arthritis group and the Han nationality control group (蠂 ~ 2 = 14.986, P < 0.001), the allele C _ (T) of the arthritis group was 112A 0.43 ~ (146N) 0.57, and the control group's OR was (0.57) ~ (0.57) ~ (th) ~ (0.43) (OR = 0.57 ~ (95) CI = 0.4200.773n 路3 respectively). The genotypic frequencies of CCT in Uygur arthritis group were 15 / 27 / 30 in IL-1 尾 -511C / T locus and 36123 / 63 in control group, respectively, and chi-square test was performed. There was significant difference between the Han nationality arthritis group and the Han nationality control group (蠂 2 / 7.119, P 0.028), the allele CnT of Uygur arthritis group was 570.40, the allele of Uygur nationality was 0.60, and the OR of Uygur control group was 1950.44 / 2490.56A / L = 0.83795CI = 0.5701.227a, respectively. Conclusion the TT genotype of IL-1 尾 -511C / T locus is highly correlated with the occurrence of Uygur arthritis. The TT genotype of TGF- 尾 1-1348C/T locus is highly correlated with the occurrence of arthritis in Han nationality.
【學位授予單位】：石河子大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R684.3

【參考文獻】

相關(guān)期刊論文 前10條

1 谷艷超;劉世清;夏韶強;廖琦;王文靜;;骨關(guān)節(jié)炎發(fā)病機制和治療的研究進展[J];中國骨與關(guān)節(jié)雜志;2016年10期

2 陳偉;呂紅芝;張曉琳;殷兵;劉松;張英澤;;中國中老年人群膝關(guān)節(jié)骨性關(guān)節(jié)炎患病率流行病學調(diào)查設(shè)計[J];河北醫(yī)科大學學報;2015年04期

3 閆虎;蘇友新;林學義;;IL-1β誘導新西蘭大白兔膝關(guān)節(jié)退變軟骨細胞的體外培養(yǎng)及鑒定[J];中國中西醫(yī)結(jié)合雜志;2014年01期

4 高金亮;李建民;孫剛;董建文;王磊;;膝骨關(guān)節(jié)炎與腰椎間盤突出癥關(guān)系的臨床研究[J];中國矯形外科雜志;2013年03期

5 樊z巖，

本文編號：1880479