本文選題：骨折 + 骨鈣素　； 參考：《中國(guó)組織工程研究》2016年51期

【摘要】：背景:對(duì)血液中骨代謝標(biāo)志物的濃度進(jìn)行檢測(cè)以對(duì)骨折愈合情況進(jìn)行評(píng)估具有使用簡(jiǎn)單、創(chuàng)傷小、變異小的特點(diǎn),如何找到一種合適的標(biāo)志物以預(yù)測(cè)骨不連的發(fā)生是當(dāng)前該領(lǐng)域研究的熱點(diǎn)。目的:建立骨折不愈合動(dòng)物模型,探討實(shí)驗(yàn)性骨折不愈合的生化指標(biāo)變化規(guī)律。方法:選取五六月齡純種新西蘭大白兔20只,隨機(jī)均分為2組,骨缺損組在左前臂橈骨中段截除1.5 cm(包括骨膜),骨斷端用骨蠟封閉髓腔;骨折組只是在前臂橈骨中段造成骨折。2組分別于術(shù)前、術(shù)后2-8,10,12周進(jìn)行前臂的X射線片檢查觀察橈骨缺損區(qū)愈合情況,并抽取血清采用生物素雙抗體夾心ELISA法檢測(cè)骨吸收特異標(biāo)志物(包括Ⅰ型膠原羧基端末肽、Ⅰ型膠原氨基端末肽、人抗酒石酸酸性磷酸酶5b)及骨形成特異標(biāo)志物(包括骨鈣素、骨特異性堿性磷酸酶)的表達(dá)變化。結(jié)果與結(jié)論:1分析2組手術(shù)前后骨代謝指標(biāo)的變化發(fā)現(xiàn),骨缺損組骨代謝一直持續(xù)在較高水平,骨不連早期診斷的臨床預(yù)測(cè)可以依賴于多個(gè)血清生化指標(biāo)的同時(shí)跟蹤監(jiān)測(cè);2除人抗酒石酸酸性磷酸酶5b意義不大之外,骨缺損組中I型膠原羧基端末肽在術(shù)后第5周均值最高,骨鈣素、骨特異性堿性磷酸酶、Ⅰ型膠原氨基端末肽在術(shù)后4或5周均值明顯下降,可以說明Ⅰ型膠原羧基端末肽、Ⅰ型膠原氨基端末肽、骨鈣素、骨特異性堿性磷酸酶能快速隨骨形成和骨吸收而迅速改變,敏感地反映體內(nèi)骨代謝的具體狀態(tài);3系統(tǒng)性監(jiān)測(cè)生化指標(biāo)如Ⅰ型膠原羧基端末肽、骨鈣素、骨特異性堿性磷酸酶、Ⅰ型膠原氨基端末肽的變化能反映兔骨折不愈合的早期進(jìn)程,它們是否均可以作為反映骨吸收與骨形成的敏感性、特異性較強(qiáng)的指標(biāo),是否可以早期診斷兔骨不連,需要進(jìn)一步深入探討。
[Abstract]:Background: the detection of bone metabolic markers in blood to assess fracture healing is characterized by simple use, minimal trauma, and little variation. How to find a suitable marker to predict the occurrence of bone nonunion is a hot topic in this field. Objective: to establish an animal model of nonunion and to investigate the changes of biochemical indexes in experimental nonunion. Methods: twenty purebred New Zealand white rabbits aged five or six months were randomly divided into two groups: the bone defect group had 1.5 cm amputation at the middle segment of the radius of the left forearm (including periosteum, bone wax was used to seal the medullary cavity at the broken end of the bone; In the fracture group, the fracture occurred in the middle radius of the forearm, and the healing of the defect area of the radius was observed by X-ray examination of the forearm before operation and 2 ~ 810 ~ (th) weeks after operation. The specific markers of bone resorption (including type I collagen carboxyl terminal peptide, type I collagen amino terminal peptide, human tartrate resistant acid phosphatase 5b) and bone formation specific markers (including osteocalcin) were detected by biotin double antibody sandwich ELISA assay. The expression of bone specific alkaline phosphatase. Results and ConclusionThe changes of bone metabolism indexes before and after operation in the two groups were analyzed by 1: 1. It was found that bone metabolism in the bone defect group had been maintained at a high level. The clinical prediction of early diagnosis of bone nonunion can depend on multiple serum biochemical indexes and follow up and monitor. Except for the significance of human tartrate resistant acid phosphatase 5b, the carboxyl terminal peptide of type I collagen was the highest in the bone defect group at the 5th week after operation. The mean value of osteocalcin, bone specific alkaline phosphatase and type 鈪，

本文編號(hào)：1858497