本文選題：髓核間質干細胞 + 脂肪間質干細胞��； 參考：《山西醫(yī)科大學》2015年碩士論文

【摘要】：目的:比較髓核間質干細胞與脂肪間質干細胞體外向類髓核細胞誘導分化能力的差異。方法:分別取大鼠腹股溝處脂肪組織與尾段脊柱,采用機械酶消化法分離培養(yǎng)AD-MSC與NP-MSC。流式細胞儀檢測兩種干細胞CD105、CD90、CD29、CD45、CD44、CD34、CD24的表達。AD-MSC與NP-MSC各自分為誘導組、無因子誘導組和對照組。誘導組以TGF-beta1標準軟骨誘導液培養(yǎng);無因子誘導組以不含TGF-beta1的軟骨誘導液培養(yǎng);對照組以含10%胎牛血清的DMEM/F12培養(yǎng)液培養(yǎng)。培養(yǎng)14d后用RT-PCR檢測各組細胞Ⅱ型膠原、蛋白多糖、SOX-9基因的表達。結果:兩種干細胞CD105、CD90、CD29表達陽性;CD45、CD44、CD34、CD24表達陰性。向類髓核細胞誘導培養(yǎng)14d后兩種細胞誘導組Collagen typeⅡ、Aggrecan、SOX-9基因表達水平較對照組均明顯升高,差異有統(tǒng)計學意義(P0.05);NP-MSC誘導組三種基因的表達水平均明顯高于AD-MSC誘導組,差異有統(tǒng)計學意義(P0.05)。結論:髓核間質干細胞與脂肪間質干細胞體外均具有向類髓核細胞分化的能力,而髓核間質干細胞可能更優(yōu)于脂肪間質干細胞,更適合于作為組織工程髓核研究的種子細胞。
[Abstract]:Aim: to compare the differentiation ability of nucleus pulposus mesenchymal stem cells and adipose mesenchymal stem cells into nucleus pulposus cells in vitro. Methods: AD-MSC and NP-MSCs were isolated from rat inguinal adipose tissue and caudal spine by mechanical enzyme digestion. Flow cytometry was used to detect the expression of CD105, CD90, CD29, CD45, CD44, CD34 and CD24 in stem cells. AD-MSC and NP-MSC were divided into three groups: induction group, non-factor induced group and control group. The induction group was cultured with TGF-beta1 standard cartilage induction medium, the non-factor induced group with TGF-beta1 free cartilage culture medium, and the control group with DMEM/F12 culture medium containing 10% fetal bovine serum. After cultured for 14 days, the expression of type 鈪，

本文編號：1827169