本文選題：髓核間質(zhì)干細(xì)胞 + 脂肪間質(zhì)干細(xì)胞�。� 參考：《山西醫(yī)科大學(xué)》2015年碩士論文

【摘要】：目的:比較髓核間質(zhì)干細(xì)胞與脂肪間質(zhì)干細(xì)胞體外向類髓核細(xì)胞誘導(dǎo)分化能力的差異。方法:分別取大鼠腹股溝處脂肪組織與尾段脊柱,采用機(jī)械酶消化法分離培養(yǎng)AD-MSC與NP-MSC。流式細(xì)胞儀檢測兩種干細(xì)胞CD105、CD90、CD29、CD45、CD44、CD34、CD24的表達(dá)。AD-MSC與NP-MSC各自分為誘導(dǎo)組、無因子誘導(dǎo)組和對(duì)照組。誘導(dǎo)組以TGF-beta1標(biāo)準(zhǔn)軟骨誘導(dǎo)液培養(yǎng);無因子誘導(dǎo)組以不含TGF-beta1的軟骨誘導(dǎo)液培養(yǎng);對(duì)照組以含10%胎牛血清的DMEM/F12培養(yǎng)液培養(yǎng)。培養(yǎng)14d后用RT-PCR檢測各組細(xì)胞Ⅱ型膠原、蛋白多糖、SOX-9基因的表達(dá)。結(jié)果:兩種干細(xì)胞CD105、CD90、CD29表達(dá)陽性;CD45、CD44、CD34、CD24表達(dá)陰性。向類髓核細(xì)胞誘導(dǎo)培養(yǎng)14d后兩種細(xì)胞誘導(dǎo)組Collagen typeⅡ、Aggrecan、SOX-9基因表達(dá)水平較對(duì)照組均明顯升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05);NP-MSC誘導(dǎo)組三種基因的表達(dá)水平均明顯高于AD-MSC誘導(dǎo)組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:髓核間質(zhì)干細(xì)胞與脂肪間質(zhì)干細(xì)胞體外均具有向類髓核細(xì)胞分化的能力,而髓核間質(zhì)干細(xì)胞可能更優(yōu)于脂肪間質(zhì)干細(xì)胞,更適合于作為組織工程髓核研究的種子細(xì)胞。
[Abstract]:Aim: to compare the differentiation ability of nucleus pulposus mesenchymal stem cells and adipose mesenchymal stem cells into nucleus pulposus cells in vitro. Methods: AD-MSC and NP-MSCs were isolated from rat inguinal adipose tissue and caudal spine by mechanical enzyme digestion. Flow cytometry was used to detect the expression of CD105, CD90, CD29, CD45, CD44, CD34 and CD24 in stem cells. AD-MSC and NP-MSC were divided into three groups: induction group, non-factor induced group and control group. The induction group was cultured with TGF-beta1 standard cartilage induction medium, the non-factor induced group with TGF-beta1 free cartilage culture medium, and the control group with DMEM/F12 culture medium containing 10% fetal bovine serum. After cultured for 14 days, the expression of type 鈪，

本文編號(hào)：1827169