本文選題：TSG-6 + 橋血管　； 參考：《安徽醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:觀察TSG-6(tumor necrosis factor alpha-stimulated gene-6)在大鼠移植靜脈壁中的表達,探討其作用及可能的機制,了解血管外支架、腫瘤壞死因子α(TNF-α)和白細胞介素-1(IL-1)對其表達的影響,為臨床治療移植后血管再狹窄提供新方向和理論依據(jù)。方法:安徽省動物中心供應(yīng)的SPF級大鼠30只,雌雄不分,體重在270-330g,隨機分成三組,即假手術(shù)組(JA)、支架保護組(ZA)和非支架保護組(WA),每組10只。JA組模型建立,游離大鼠右側(cè)頸外靜脈后,原位保留,縫合切口皮下組織及皮膚;WA組模型建立,采用袖套法(改良cuff法),取大鼠自體頸外靜脈長約1.5cm,移植到同側(cè)頸總動脈,建立血管移植模型;ZA組模型建立,在WA組手術(shù)操作中對移植靜脈血管植入血管外支架(20G靜脈留置穿刺針尾端輸液管),預(yù)防移植血管的過度擴張。2周和8周后分別對ZA組和JA組行血管彩超檢查,評估血管通暢情況。術(shù)后大鼠統(tǒng)一飼養(yǎng),阿司匹林腸溶片10mg/kg/d灌胃,預(yù)防血栓形成。三組大鼠模型飼養(yǎng)12周,原切口切開取材。標(biāo)本分兩份,一份立即置于-80℃冰箱里冷凍,另一份以10%多聚甲醛固定。應(yīng)用蘇木精-伊紅觀察移植靜脈內(nèi)、中、外膜增生情況,采用計算機圖像分析系統(tǒng)測量和計算各膜厚度;應(yīng)用Western-blot檢測移植靜脈壁細胞內(nèi)TSG-6、TNF-α和IL-1的表達。結(jié)果:30只大鼠無死亡,術(shù)后2周血管彩超各組動物模型血管通暢,血流速達15cm/s。術(shù)后8周血管彩超提示ZA組和WA組各有一只大鼠移植血管血流信號差,血管堵塞不通,總血管通暢率達93.3%。取材時肉眼觀察:JA組血管與正常頸外靜脈損傷血管無明顯改變;ZA組血管支架外纖維組織包裹,受支架保護血管外膜較光滑,血管壁較JA組增厚;WA組血管囊袋狀擴張明顯,動脈搏動明顯,外膜與周圍組織有粘連,較ZA組血管壁增厚顯著,WA組與ZA組相比較,內(nèi)膜、中層、外膜增生分別達到1.5倍、1.7倍和1.8倍。HE染色顯示,血管壁三層界限清晰WA組與JA組相比,血管壁各層增生明顯,以中層為著;TSG-6在JA組中的表達比手術(shù)組(ZA組和WA組)中表達低;TSG-6在JA組中表達低于低于支架保護組P0.05,TSG-6在ZA組中表達低于WA組(P0.001);炎癥因子TNF-α和IL-1的表達與TSG-6呈正相關(guān),在ZA組的TNF-α和IL-1表達水平均顯著低于WA組(P0.05)。結(jié)論:1)建立大鼠頸靜脈移植動物模型,套管法簡單、易操作;2)TSG-6、TNF-α、IL-1在大鼠靜脈移植靜脈血管壁細胞中存在表達;3)TSG-6與TNF-α、IL-1炎癥因子一起參與血管的炎癥反應(yīng)和內(nèi)膜增生;4)血管外限制性支架能有效抑制血管壁細胞增生,控制移植靜脈的過度擴張,預(yù)防靜脈血管壁細胞過度增生所致血管再狹窄。
[Abstract]:Objective: to observe the expression of TSG-6(tumor necrosis factor alpha-stimulated gene-6 in the vein wall of rats, and to investigate the effects of extracellular stents, tumor necrosis factor 偽 -TNF- 偽 (TNF- 偽) and interleukin-1 (IL-1) on the expression of TSG-6(tumor necrosis factor alpha-stimulated gene-6. To provide a new direction and theoretical basis for clinical treatment of vascular restenosis after transplantation. Methods: thirty SPF rats, male and female, weighing 270-330 g, supplied by Anhui Animal Center, were randomly divided into three groups: sham-operated group, stent-protected group and non-stenting protection group, with 10 rats in each group. After the right external jugular vein was free, the right jugular vein was preserved in situ, and the subcutaneous tissue and skin were sutured to establish the model of WA group. The cuff method (modified cuff method) was used. The length of the external jugular vein was about 1.5 cm, and transplanted to the ipsilateral common carotid artery. The model of blood vessel transplantation was established in group ZA. During the operation of WA group, 20 G vein indwelling catheter was inserted into the vein to prevent the excessive dilatation of the transplanted blood vessel at the end of 2 weeks and 8 weeks after the operation, the blood vessels in group ZA and group JA were examined by color Doppler ultrasound, respectively. To evaluate the patency of blood vessels. After operation, rats were fed with aspirin enteric-coated tablets (10mg/kg/d) to prevent thrombosis. Three groups of rats were fed for 12 weeks. The specimen is divided into two parts, one is immediately frozen in-80 鈩，

本文編號：1805772