本文選題：糖尿病 + 骨折延遲愈合　； 參考：《中國老年學(xué)雜志》2017年15期

【摘要】：目的探討骨形態(tài)發(fā)生蛋白(BMP)-2、胰島素生長因子(IGF)-1基因治療糖尿病(DM)骨折延遲愈合的作用和機(jī)制。方法選取6~7周齡雄性Wistar大鼠150只,分為骨折對照組、DM骨折組和治療組。利用酶聯(lián)免疫試劑盒檢測各組大鼠血清中BMP-2、IGF-1的表達(dá);測定各組大鼠血清中堿性磷酸酶(ALP)的含量,以判斷對成骨細(xì)胞生長的影響;利用放射免疫競爭法檢測血清中骨鈣素的含量變化;利用局部骨密度儀檢測骨折區(qū)域局部骨密度的變化情況。結(jié)果術(shù)后前3 w,DM骨折組與骨折對照組BMP-2、IGF-1的濃度均呈上升趨勢,第3周達(dá)到最大值,治療組BMP-2含量在第2周達(dá)到最大值,IGF-1的濃度在第3周達(dá)到最大,隨后開始下降、實(shí)驗(yàn)中,治療組BMP-2、IGF-1的表達(dá)量最高,DM骨折組表達(dá)量始終最低。DM骨折組ALP與骨鈣素的含量始終顯著低于骨折對照組與治療組,骨折前3 w,治療組ALP與骨鈣素的含量顯著低于對照組骨折無顯著差異(P0.05)。骨折區(qū)域局部骨密度檢測結(jié)果顯示治療組骨密度最高,骨折對照組其次,DM骨折組骨密度最低。結(jié)論 BMP-2、IGF-1基因通過提高DM大鼠骨折愈合過程中與成骨細(xì)胞增殖成熟相關(guān)的細(xì)胞因子的表達(dá),促進(jìn)骨折愈合過程中骨折區(qū)域局部骨密度的增加,從而顯著改善DM骨折延遲愈合情況。
[Abstract]:Objective to investigate the effect and mechanism of bone morphogenetic protein BMP-2, IGF-1 gene on delayed healing of DM fracture. Methods 150 male Wistar rats aged 6 weeks and 7 weeks were divided into two groups: fracture group and treatment group. The expression of BMP-2TIGF-1 in serum and the content of alkaline phosphatase (ALP) in serum of rats in each group were detected by enzyme-linked immunosorbent assay (Elisa) to determine the effect on the growth of osteoblasts. The changes of serum osteocalcin and bone mineral density in fracture area were detected by radioimmunoassay. Results the concentration of BMP-2 IGF-1 in the DM fracture group and the fracture control group increased in the first 3 weeks after operation and reached the maximum at the third week. The BMP-2 content in the treatment group reached the maximum at the second week and then began to decrease. The expression of BMP-2 and IGF-1 was the highest in the treatment group and the lowest in the DM fracture group. The contents of ALP and osteocalcin in the DM fracture group were significantly lower than those in the fracture control group and the treatment group. The levels of ALP and osteocalcin in the treatment group were significantly lower than those in the control group 3 weeks before fracture (P 0.05). The local bone mineral density in fracture area was the highest in the treatment group and the lowest in the DM fracture group in the fracture control group. Conclusion BMP-2 + IGF-1 gene enhances the expression of cytokines related to the proliferation and maturation of osteoblasts in the process of fracture healing in DM rats, and promotes the increase of bone mineral density in the fracture region during fracture healing. The delayed union of DM fracture was improved significantly.
【作者單位】： 杭州市解放軍117醫(yī)院骨科;安徽醫(yī)科大學(xué)解放軍九八臨床學(xué)院;
【基金】：浙江省自然科學(xué)基金資助項(xiàng)目(No.Y2080991)
【分類號】：R587.2;R683
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本文編號：1802420