本文選題：皮膚撕脫傷 + 冷藏皮　； 參考：《中國老年學(xué)雜志》2017年19期

【摘要】：目的對比研究冷藏皮和反植皮的成活率及冷藏皮和液氮冷凍皮膚的病理學(xué)變化。方法分析冷藏皮延遲植皮的成活率及病理學(xué)變化情況,并對冷藏皮和液氮冷凍皮進(jìn)行病理形態(tài)學(xué)和細(xì)胞凋亡率觀察。結(jié)果動物實(shí)驗(yàn)研究表明,即時(shí)回植組與冷藏皮24 h、48 h組成活率無統(tǒng)計(jì)學(xué)差異(P0.05);冷藏皮72 h、120 h、168 h回植組成活率明顯低于即時(shí)回植組(P0.05),提示冷藏皮回植黃金時(shí)間為48 h以內(nèi)。大鼠皮膚細(xì)胞空泡變性率比較,冷藏皮在第1,3,5,10天的空泡變性率均高于即時(shí)回植組(P0.05),但第1天的空泡變性率接近即時(shí)回植組,而第3,5,10天的空泡變性率與即時(shí)回植組差距較大。液氮組第1,3,5,10天的空泡變性率也高于即時(shí)回植組(P0.05),但是空泡變性率均較接近即時(shí)回植組,組間絕對數(shù)差距也較冷藏組的組間差距小。大鼠皮膚細(xì)胞凋亡率比較,冷藏皮在第1,3,5,10天的凋亡率均高于即時(shí)回植組(P0.05),但第1、3天的凋亡率無統(tǒng)計(jì)學(xué)差異(P0.05),而與第5、10天的凋亡率差距較大。液氮組的第1,3,5,10天的凋亡率明顯高于即時(shí)回植組(P0.05),凋亡率也明顯高于冷藏組。結(jié)論皮膚冷藏48 h以內(nèi)為回植的安全期限,液氮冷藏皮膚,細(xì)胞變性率低,但是凍存和解凍過程可能導(dǎo)致細(xì)胞凋亡率增加。
[Abstract]:Objective to study the survival rate of cold storage skin and anti-graft skin and the pathological changes of refrigerated skin and liquid nitrogen frozen skin. Methods the survival rate and pathological changes of frozen skin and liquid nitrogen frozen skin were analyzed, and the pathomorphology and apoptosis rate of frozen skin and liquid nitrogen frozen skin were observed. Results Animal experiments showed that there was no significant difference in survival rate between the immediate replanting group and the cold storage skin group at 24 h and 48 h, and the survival rate of the cold storage skin group was significantly lower than that of the immediate replanting group within 48 h. The survival rate of the cold storage skin group was significantly lower than that of the immediate replanting group within 48 h. Compared with the denaturation rate of skin cells, the denaturation rate of frozen skin on day 10 was higher than that of immediate replantation group (P 0.05), but the rate of denaturation of frozen skin was close to that of immediate replantation group on day 1, but the rate of denaturation of vacuolation on day 3 510 was larger than that of group B. The denaturation rate of vacuolation in liquid nitrogen group was also higher than that in immediate replantation group on the 10th day, but the denaturation rate of cavitation was similar to that of immediate replantation group, and the absolute difference between groups was also smaller than that in cold storage group. The apoptotic rate of skin cells in the cold storage skin group was higher than that in the immediate replantation group on the 10th day, but there was no significant difference in the apoptosis rate on the 1st day and the 3rd day, but there was a big difference between the cold storage skin and the 5th day. The apoptosis rate of liquid nitrogen group was significantly higher than that of immediate replantation group on the 10th day, and the apoptotic rate was significantly higher than that of cold storage group. Conclusion the safe time of skin replantation is within 48 h, and the denaturation rate of skin is low when liquid nitrogen is refrigerated, but the rate of apoptosis may be increased during freezing and thawing.
【作者單位】： 鄭州市第一人民醫(yī)院骨外科;濟(jì)南軍區(qū)總醫(yī)院骨外科;濟(jì)南市婦幼保健院桿石橋社區(qū)服務(wù)中心;山東大學(xué)齊魯醫(yī)院皮膚科;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(No.30973018)
【分類號】：R622.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 孫同柱,吳志谷,付小兵,陳偉,胥照平,戴紅梅,朱厚礎(chǔ);重組人表皮生長因子對大鼠皮膚生長的影響[J];中華實(shí)驗(yàn)外科雜志;2003年07期

2 楊磊;高建華;王穎;王甲漢;;Ⅰ型胰島素樣生長因子重組真核表達(dá)載體的構(gòu)建及在燙傷大鼠皮膚中的表達(dá)[J];中國臨床康復(fù);2006年20期

3 楊磊;高建華;王甲漢;;單純皰疹病毒胸苷激酶基因重組真核表達(dá)載體的構(gòu)建及在燙傷大鼠皮膚組織中的表達(dá)[J];中國臨床康復(fù);2006年12期

4 吳本s，

本文編號：1783403