本文選題：人臍帶間充質(zhì)干細(xì)胞 + 富含血小板血漿�。� 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2017年02期

【摘要】：目的探討不同濃度富含血小板血漿(platelet-rich plasma,PRP)在體外對(duì)人臍帶間充質(zhì)干細(xì)胞(human umbilical cord mesenchymal stem cells,h UC-MSCs)增殖、分化的影響,評(píng)估h UC-MSCs聯(lián)合PRP對(duì)大鼠跟腱損傷修復(fù)的影響。方法 PRP活化后成為PRP-clot releasate(PRCR)。取P3代h UC-MSCs于培養(yǎng)皿中培養(yǎng)24 h后,分別加入含0%(對(duì)照組)、10%、20%、40%PRCR的DMEM/F12培養(yǎng)基,于第1、2、3、4天測(cè)定h UC-MSCs的增殖速度。P3代h UC-MSCs按相同分組培養(yǎng),于第1、3、5天收集細(xì)胞并提取蛋白。Western blot檢測(cè)h UC-MSCs中成肌腱分化標(biāo)志性基因肌腱蛋白C(tenascin c,TNC)、SCX(scleraxis)、Ⅰ型膠原的表達(dá)。選取48只8周齡雄性SD大鼠,Ⅰ型膠原酶溶液50μL注射于大鼠右側(cè)跟腱制備跟腱損傷模型,3 d后于損傷部位再次分別注射50μL生理鹽水(對(duì)照)、h UC-MSCs懸液、PRP或PRP+h UC-MSCs混懸液,術(shù)后2、4周安樂死大鼠,Western blot檢測(cè)跟腱中TNC、SCX及Ⅰ型膠原的表達(dá),跟腱切片HE染色行組織學(xué)評(píng)估。結(jié)果 PRCR在體外對(duì)h UC-MSCs增殖均有促進(jìn)作用,20%PRCR組促增殖作用最顯著(P0.05);20%PRCR組較對(duì)照組在體外可以顯著地促進(jìn)h UCMSCs成肌腱分化標(biāo)志性基因的表達(dá)(P0.05);術(shù)后2、4周,PRP+h UC-MSCs組跟腱中成肌腱分化相關(guān)基因在蛋白水平表達(dá)更高,跟腱切片HE染色顯示PRP+h UC-MSCs組纖維排列更規(guī)整,炎細(xì)胞更少。結(jié)論適宜濃度PRP在體外顯著促進(jìn)了h UC-MSCs的增殖、成肌腱分化;PRP聯(lián)合h UC-MSCs能有效促進(jìn)大鼠受損跟腱的修復(fù)。
[Abstract]:Objective to investigate the effects of platelet-rich plasma-rich plasma with different concentrations of platelet-rich plasma on proliferation and differentiation of human umbilical cord mesenchymal stem cells hUC-MSCs in vitro, and to evaluate the effects of h UC-MSCs combined with PRP on the repair of Achilles tendon injury in rats. Methods PRP was activated to become PRP-clot release. After cultured in culture dish for 24 hours, P3 generation h UC-MSCs was added into DMEM/F12 medium containing 0 (control group 10) and 20% 40 PRCR respectively. The proliferation rate of h UC-MSCs. P3 generation h UC-MSCs were cultured according to the same group on day 1, 2, 3 and 3, 4 days later. The cells were collected on the 3rd day of the first trimester, and the protein was extracted. Western blot was used to detect the expression of the tendon protein (C(tenascin), a typical gene of tendon differentiation in h UC-MSCs, SCX scleraxisus, type 鈪，

本文編號(hào)：1781375