本文選題：雙光子成像 + 樹突棘　； 參考：《中國人民解放軍醫(yī)學院》2015年碩士論文

【摘要】：目的脊髓損傷可以導致多層次的中樞神經(jīng)系統(tǒng)的重塑,其中就包括大腦皮層。本實驗使用雙光子顯微鏡活體觀察的方法探究脊髓半切后小鼠大腦雙側(cè)后肢感覺和運動皮層樹突棘和軸突膨體的變化以探討脊髓損傷后皮層的重塑問題。方法將40只YFP小鼠隨機分為兩大組：感覺皮層組和運動皮層組,每組20只,每大組再隨機分為兩小組：對照組和脊髓損傷組(SCI組),每組10只,對照組切除椎板不損傷脊髓,脊髓損傷組切除椎板同時半切左側(cè)胸段脊髓(T12)。分別在脊髓損傷前1天、損傷后3天(3d)、2周(2w)和1月(1m)時對兩側(cè)后肢感覺皮層(感覺皮層組)和運動皮層(運動皮層組)區(qū)域各成像四次。通過對同一位置的同一樹突棘或軸突膨體(運動皮層)在不同時間點圖像的對比,統(tǒng)計出新形成、消失和穩(wěn)定存在的樹突棘或軸突膨體(運動皮層)的數(shù)量,計算變化的概率以及和時間的關(guān)系,同時計算出穩(wěn)定、消失、新形成的樹突棘在以后較長時間段的存活率。結(jié)果感覺皮層重塑方面,與對照組相比,SCI組半切后3d和2w時對側(cè)感覺皮層新形成的樹突棘明顯增加,雙側(cè)感覺皮層在脊髓半切后2w和1m時消失的樹突棘明顯增多,穩(wěn)定的樹突棘數(shù)量明顯減少。在損傷后3d到2w期間,SCI組雙側(cè)皮層的原本穩(wěn)定的樹突棘的存活率明顯下降。與SCI組對側(cè)皮層和對照組相比,SCI組同側(cè)皮層消失的樹突棘重新出現(xiàn)的概率下降。在損傷后2w到1m期間,SCI組雙側(cè)皮層新形成的樹突棘的存活率下降。運動皮層重塑方面,與對照組相比,SCI組半切后在1個月內(nèi)兩側(cè)運動皮層軸突膨體并無明顯變化;而在樹突棘方面,與對照組相比,SCI組雙側(cè)運動皮層在脊髓半切后2w時消失的樹突棘明顯增多,穩(wěn)定的樹突棘數(shù)量明顯減少,半切后1m時對側(cè)運動皮層新形成的樹突棘明顯增加。在損傷后3d到2w期間,SCI組雙側(cè)運動皮層的原本穩(wěn)定的樹突棘的存活率明顯下降,消失的樹突棘重新出現(xiàn)的概率下降。在損傷后2w到lm期間,SCI組對側(cè)運動皮層新形成的樹突棘的存活率下降。結(jié)論脊髓半切后小鼠后肢感覺和運動皮層均發(fā)生重塑,重塑發(fā)生在兩側(cè)皮層。重塑不僅涉及樹突棘的減少,也存在大量新形成的樹突棘。對于運動皮層來說,皮層中突觸的重塑主要發(fā)生在突觸后組成部分樹突棘而非突觸前組成部分軸突膨體。在脊髓損傷后前期,感覺皮層重塑主要發(fā)生在半切對側(cè)皮層,以樹突棘新形成為主;其后重塑便涉及雙側(cè)皮層,以樹突棘消失為主。脊髓損傷后,感覺和運動皮層以往穩(wěn)定的樹突棘不再穩(wěn)定,消失后的樹突棘的重新出現(xiàn)的概率下降,新形成的樹突棘的存活率同樣下降。
[Abstract]:Objective Spinal cord injury can lead to multiple levels of central nervous system remodeling, including the cerebral cortex. In order to explore the remodeling of cortex after spinal cord injury (sci), the changes of dendritic spine and axonal bulge in bilateral posterior limb and motor cortex of mice after spinal cord hemisectomy were investigated by using two-photon microscope in vivo. Methods 40 YFP mice were randomly divided into two groups: sensory cortex group (n = 20) and motor cortex group (n = 20). Each group was randomly divided into two groups: control group (n = 10) and spinal cord injury group (n = 10). In the spinal cord injury group, the vertebral lamina was excised and the left thoracic spinal cord was cut half. The sensory cortex (sensory cortex group) and motor cortex (motor cortex group) of bilateral hind limbs were respectively imaged four times before 1 day, 3 days after injury for 2 weeks and 1 month for 1 m. By comparing the images of the same dendritic spine or axonal bulge (motor cortex) in the same position at different time points, the number of newly formed, disappeared and stable dendritic spine or axocele (motor cortex) was calculated. The probability of change and the relationship between time and change were calculated, and the survival rate of the newly formed dendritic spine in a longer period of time was calculated. Results in the sensory cortex remodeling, compared with the control group, the newly formed dendritic spine in contralateral sensory cortex increased significantly 3 days and 2 weeks after hemisection in sci group, and the dendritic spine disappeared in bilateral sensory cortex at 2 weeks and 1 m after spinal cord hemisection. The number of stable dendritic spine decreased significantly. The survival rate of the previously stable dendritic spine in bilateral cortex of sci group decreased significantly between 3 days and 2 weeks after injury. Compared with the contralateral cortex of SCI group and control group, the probability of reappearance of dendritic spine disappeared in ipsilateral cortex of sci group was decreased. The survival rate of the newly formed dendritic spine in sci group decreased from 2 weeks to 1 m after injury. In motor cortex remodeling, there was no significant change in axonal bulge of bilateral motor cortex in sci group within 1 month after hemisection compared with control group, but in dendritic spine, there was no significant change in axonal bulge in bilateral motor cortex in sci group. Compared with the control group, the number of dendritic spine disappeared and the number of stable dendritic spine decreased significantly at 2 weeks after spinal cord hemisection in sci group, and the newly formed dendritic spine in contralateral motor cortex increased significantly at 1 m after hemisection. The survival rate of the previously stable dendritic spine in the bilateral motor cortex of the sci group decreased significantly from 3 days to 2 weeks after injury, and the probability of reappearance of the disappeared dendritic spine decreased. The survival rate of the newly formed dendritic spine in the contralateral motor cortex decreased between 2 weeks and 1 m after injury in the sci group. Conclusion the sensory and motor cortex of the hind limb were remodeled after hemisection of spinal cord in mice, and the remodeling occurred in the bilateral cortex. Remodeling involves not only the reduction of dendritic spine, but also a large number of newly formed dendritic spine. For motor cortex, synaptic remodeling occurs mainly in postsynaptic dendritic spine rather than presynaptic axonocele. In the early stage of spinal cord injury, sensory cortex remodeling mainly occurred in the half-cut contralateral cortex, mainly as dendritic spine, and then the remodeling involved bilateral cortex, mainly the disappearance of dendritic spine. After spinal cord injury, the previously stable dendritic spine of sensory and motor cortex was no longer stable, and the probability of reappearance of the disappeared dendritic spine was decreased, and the survival rate of newly formed dendritic spine was also decreased.
【學位授予單位】：中國人民解放軍醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R651.2

【參考文獻】

相關(guān)期刊論文 前1條

1 MOHAMMAD FAROOQUE,JONAS ISAKSSON,YNGVE OLSSON;Changes in Synapses and Axons Demonstrated by Synaptophysin Immunohistochemistry Following Spinal Cord Compression Trauma in the Rat and Mouse[J];Biomedical and Environmental Sciences;2004年03期

，

本文編號：1776277