本文選題：六磷酸肌醇(IP) + 骨肉瘤細(xì)胞株��； 參考：《西安交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年04期

【摘要】：目的研究六磷酸肌醇(inositol hexaphosphate,IP6)對(duì)人骨肉瘤細(xì)胞株MG-63和原代培養(yǎng)成骨細(xì)胞增殖的影響,并探求其抗癌效果的最佳作用濃度。方法原代培養(yǎng)人成骨細(xì)胞并鑒定,復(fù)蘇并常規(guī)培養(yǎng)人骨肉瘤細(xì)胞株MG-63,MTT法檢測(cè)不同濃度IP6干預(yù)培養(yǎng)時(shí)兩種細(xì)胞的增殖情況,確定IP6的最佳抑癌濃度,并進(jìn)一步用流式細(xì)胞儀檢測(cè)MG-63細(xì)胞周期及凋亡情況。結(jié)果當(dāng)IP6濃度≥1mmol/L時(shí)開始抑制MG-63細(xì)胞增殖,在4mmol/L時(shí)達(dá)到最大,并呈時(shí)間-劑量依賴性;當(dāng)IP6濃度為0.5、1mmol/L時(shí),對(duì)成骨細(xì)胞增殖抑制不明顯,2mmol/L時(shí)輕度抑制,4mmol/L時(shí)導(dǎo)致成骨細(xì)胞凋亡。結(jié)論 IP6能夠抑制人骨肉瘤細(xì)胞MG-63的增殖并導(dǎo)致其凋亡,其抗癌的最佳作用濃度為2mmol/L。
[Abstract]:Objective to study the effects of inositol hexaphosphate IP6) on the proliferation of human osteosarcoma cell line MG-63 and primary cultured osteoblasts, and to find out the best concentration of its anticancer effect.Methods Primary culture of human osteoblasts and identification, resuscitation and routine culture of human osteosarcoma cell line MG-63 were used to detect the proliferation of human osteosarcoma cells treated with different concentrations of IP6, and to determine the best inhibitory concentration of IP6.Furthermore, MG-63 cell cycle and apoptosis were detected by flow cytometry.Results when the concentration of IP6 鈮，

本文編號(hào)：1770104