本文選題：雌激素 + 脊髓缺血　； 參考：《中國(guó)現(xiàn)代醫(yī)學(xué)雜志》2017年16期

【摘要】：目的研究雌激素對(duì)脊髓缺血再灌注損傷保護(hù)作用的機(jī)制。方法按隨機(jī)數(shù)表字法把成年的新西蘭大白兔分為A~E 5組,每組9只。鉗夾腎下腹主動(dòng)脈20 min恢復(fù)再灌注;B組,接受與A組同樣的麻醉和手術(shù)準(zhǔn)備,但不行缺血/再灌注損傷;C組,再灌注開(kāi)始時(shí)從兔耳的緣靜脈注射200μg/kg雌激素;D組,再灌注開(kāi)始時(shí)從兔耳的緣靜脈注射400μg/kg雌激素;E組,再灌注開(kāi)始時(shí)從兔耳的緣靜脈注射800μg/kg雌激素。再灌注48 h后根據(jù)Tarlov法對(duì)下肢的神經(jīng)功能進(jìn)行評(píng)分,然后取脊髓組織蘇木素-伊紅染色法(HE)進(jìn)行病理分析。結(jié)果 Tarlov評(píng)分中雌激素組的神經(jīng)功能評(píng)分高于A組,與A組比較有統(tǒng)計(jì)學(xué)意義(P0.05)。組織病理表明脊髓的前角運(yùn)動(dòng)神經(jīng)元出現(xiàn)凋亡,有顯著壞死。A組脊髓的前角運(yùn)動(dòng)神經(jīng)元計(jì)數(shù)少于雌激素組,兩者比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論雌激素可明顯改善兔脊髓缺血再灌注的下肢神經(jīng)功能,使脊髓的前角正常運(yùn)動(dòng)神經(jīng)元的數(shù)量增加,減小缺血再灌注的損傷。
[Abstract]:Objective to study the protective effect of estrogen on spinal cord ischemia reperfusion injury.Methods the adult New Zealand white rabbits were divided into five groups according to the random numeration method, 9 rabbits in each group.Group B received the same anesthesia and operation preparation as group A, but no ischemia / reperfusion injury. Group D received 200 渭 g/kg estrogen at the beginning of reperfusion.At the beginning of reperfusion, 400 渭 g/kg estrogen was injected into the marginal vein of rabbit ear and 800 渭 g/kg estrogen was injected into the vein of rabbit ear at the beginning of reperfusion.After 48 hours of reperfusion, the nerve function of lower extremity was assessed by Tarlov method, and then the histopathological analysis was performed by hematoxylin-eosin staining in spinal cord tissue.Results the neurological function score of estrogen group was higher than that of A group in Tarlov score, which was significantly higher than that in A group (P 0.05).Histopathology showed that the number of anterior horn motor neurons in group A was less than that in estrogen group, and there was significant difference between the two groups (P 0.05).Conclusion estrogen can obviously improve the nerve function of lower extremity after spinal cord ischemia reperfusion, increase the number of normal motor neurons in the anterior horn of spinal cord, and reduce the injury of ischemia reperfusion.
【作者單位】： 南華大學(xué)附屬第一醫(yī)院乳腺甲狀腺外科;南華大學(xué)醫(yī)學(xué)院外科學(xué)教研室;
【分類(lèi)號(hào)】：R651.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 區(qū)泳芳;伏學(xué)坤;梅興莎;鄭惠珍;;三七總皂苷對(duì)大鼠骨髓間充質(zhì)干細(xì)胞凋亡的抑制作用[J];生理學(xué)報(bào);2016年03期

2 劉敬霞;任非非;劉會(huì)賢;劉洋;李娟;虎喜成;;扎里奴思方干預(yù)骨髓間充質(zhì)干細(xì)胞移植對(duì)腦缺血再灌注損傷大鼠黏附分子及NeuN表達(dá)的影響[J];中國(guó)實(shí)驗(yàn)方劑學(xué)雜志;2016年12期

3 李映辰;王瑾茜;劉侃;胡國(guó)恒;;腎腦復(fù)元湯聯(lián)合人臍帶間充質(zhì)干細(xì)胞移植對(duì)腦缺血再灌注大鼠神經(jīng)營(yíng)養(yǎng)因子表達(dá)的影響[J];中草藥;2016年05期

4 屈新輝;王萬(wàn)松;吳凌峰;謝琛;張昆南;吳曉牧;;動(dòng)、靜脈移植骨髓間充質(zhì)干細(xì)胞治療急性期缺血再灌注大鼠模型的比較[J];中國(guó)老年學(xué)雜志;2015年24期

5 司曉蕓;李靜靜;畢曉紅;吳小燕;;骨髓間充質(zhì)干細(xì)胞表面CXCR4過(guò)表達(dá)影響缺血-再灌注大鼠腎損傷修復(fù)的機(jī)制研究[J];中華急診醫(yī)學(xué)雜志;2015年12期

6 林海泓;羅心平;施海明;龔輝;史益軍;;促紅細(xì)胞生成素基因修飾對(duì)H_2O_2誘導(dǎo)的小鼠骨髓間充質(zhì)干細(xì)胞凋亡的影響[J];中國(guó)分子心臟病學(xué)雜志;2015年03期

7 戚朦;劉文佳;馬洋;楊q，

本文編號(hào)：1736854