發(fā)布時(shí)間：2018-04-10 07:25

  本文選題：胰腺創(chuàng)傷模型　切入點(diǎn)：損傷面積　出處：《山東醫(yī)藥》2017年25期

【摘要】：目的探討建立一種新型大鼠單純胰腺創(chuàng)傷模型,并基于損傷面積評(píng)估其傷情變化。方法將大鼠隨機(jī)分為2組,采用自主研發(fā)多功能撞擊儀的3 cm~2(3 cm~2致傷面積組)和6 cm~2(6 cm~2致傷面積組)撞擊頭,分別擠壓各組處于開腹?fàn)顟B(tài)的胰腺,使之在400 k Pa的壓強(qiáng)下形成不同面積的損傷,并設(shè)置對(duì)照組;建模24 h后觀察各組大鼠存活率、一般情況、大體病理和組織病理學(xué)改變,并檢測(cè)血鉀、血鈣的濃度和血清、腹水中淀粉酶及脂肪酶的活性。結(jié)果與對(duì)照組比較,3 cm~2和6 cm~2致傷面積組均能形成明顯的損傷(P0.05),其中,與3 cm~2致傷面積組比較,6 cm~2致傷面積組損傷更顯著(P0.05)。與3 cm~2致傷面積組相比,6 cm~2致傷面積組大鼠存活率、血鉀及血鈣水平更低(P均0.05),一般情況更差,損傷性病理改變的范圍及程度更大,血清淀粉酶、脂肪酶更高(P均0.05)。結(jié)論成功建立了一種基于損傷面積控制的大鼠胰腺創(chuàng)傷模型。該模型簡(jiǎn)單、有效、可控性及可重復(fù)性好,適合用于傷情演化機(jī)制和創(chuàng)傷施救等方面的研究。
[Abstract]:Objective to establish a new simple pancreatic trauma model in rats and to evaluate the injury status based on the injury area.Methods the rats were randomly divided into two groups. The rats were randomly divided into two groups. The rats were randomly divided into two groups. The rats were randomly divided into two groups: the 3 cm~2(3 cm~2 injury area group (3 cm~2(3 cm~2 group) and the 6 cm~2(6 cm~2 injury area group (6 cm~2(6 cm~2 group).After 24 hours of modeling, the survival rate, general condition, gross pathological and histopathological changes of rats were observed, and the concentrations of blood potassium, serum calcium and serum were measured.Activities of amylase and lipase in ascites.Results compared with the control group, the injury area of 3 cm~2 and 6 cm~2 could form significant injury P0.05A, among which, compared with the 3 cm~2 group, the injury of 6 cm~2 group was more significant than that of the 6 cm~2 group.Compared with 3 cm~2 injury area group, the survival rate, blood potassium and blood calcium levels of rats in the 6 cm~2 injury area group were lower (P < 0.05), the general condition was worse, the range and extent of pathological changes were larger, the serum amylase and lipase levels were higher than those in the control group (P < 0.05).Conclusion A rat pancreatic trauma model based on area control was successfully established.The model is simple, effective, controllable and reproducible. It is suitable for the study of injury evolution mechanism and wound rescue.
【作者單位】： 西南醫(yī)科大學(xué);成都軍區(qū)總醫(yī)院全軍普外中心;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(81001695) 四川省科技廳應(yīng)用基礎(chǔ)研究項(xiàng)目(2013JY0046)
【分類號(hào)】：R-332;R657.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳小莉,賈鵬松,張勇;胰腺創(chuàng)傷治療28例[J];河南醫(yī)藥信息;2001年18期

2 李承鋼;胰腺創(chuàng)傷的術(shù)式選擇(附17例)[J];職業(yè)衛(wèi)生與病傷;2003年03期

3 陳光安,公保才旦,馬文飚,逯克棟,張建濤;胰腺創(chuàng)傷44例的外科治療[J];青海醫(yī)藥雜志;2004年08期

4 陳元清;公保才旦;陳光安;;44例胰腺創(chuàng)傷的診斷與治療[J];青海醫(yī)藥雜志;2006年10期

5 王煒鵬;;胰腺創(chuàng)傷的診斷與治療經(jīng)驗(yàn)[J];河南外科學(xué)雜志;2010年05期

6 晉林;;胰腺創(chuàng)傷12例臨床診治體會(huì)[J];基層醫(yī)學(xué)論壇;2011年11期

7 李建鋼;;胰腺創(chuàng)傷163例臨床分析[J];中國鄉(xiāng)村醫(yī)藥;2013年08期

8 呂海旭;鐘翔宇;崔云甫;;胰腺創(chuàng)傷的診斷與治療[J];世界華人消化雜志;2013年19期

9 何國寶;王志鴻;黃國南;吳建山;;胰腺創(chuàng)傷13例診斷與治療體會(huì)[J];基層醫(yī)學(xué)論壇;2013年28期

10 劉君毅;;影響胰腺創(chuàng)傷療效的因素[J];國外醫(yī)學(xué).創(chuàng)傷與外科基本問題分冊(cè);1985年01期

相關(guān)會(huì)議論文 前1條

1 陳元清;公保才旦;陳光安;;44例胰腺創(chuàng)傷的診斷與治療[A];第十一次全國急診醫(yī)學(xué)學(xué)術(shù)會(huì)議暨中華醫(yī)學(xué)會(huì)急診醫(yī)學(xué)分會(huì)成立二十周年慶典論文匯編[C];2006年

相關(guān)博士學(xué)位論文 前1條

1 戴睿武;胰腺創(chuàng)傷Wistar大鼠模型傷情變化特點(diǎn)及Variabilin對(duì)模型PLA_2的抑制效應(yīng)及保護(hù)作用的實(shí)驗(yàn)研究[D];第三軍醫(yī)大學(xué);2007年

，

本文編號(hào)：1730247