本文選題：川芎嗪　切入點(diǎn)：神經(jīng)痛　出處：《蘭州大學(xué)》2015年碩士論文

【摘要】：目的：評價川芎嗪對慢性坐骨神經(jīng)壓迫性損傷大鼠的脊髓背根神經(jīng)節(jié)GAP-43 mRNA表達(dá)的影響,初步探討川芎嗪對神經(jīng)病理性疼痛大鼠的作用和相關(guān)的機(jī)制。方法：選取成年雄性健康Wistar大鼠共54只,周齡6～8周,體重180～220 g。分組方法采用隨機(jī)數(shù)字表法,即將54只大鼠分為3組(n=18)：假手術(shù)組(S組)、神經(jīng)病理性疼痛組(NP組)和川芎嗪組(TMP組)。NP組和TMP組采用慢性坐骨神經(jīng)壓迫損傷法建立神經(jīng)病理性疼痛模型,S組大鼠僅暴露坐骨神經(jīng)不結(jié)扎。TMP組于CCI術(shù)畢即刻腹腔注射川芎嗪100mg·kg-1,1天1次,而S組和NP組給予等容量生理鹽水腹腔注射,連續(xù)給藥至處死當(dāng)天。S組、NP組和TMP組分別隨機(jī)取6只大鼠,于術(shù)前1 d,術(shù)后3、7、14 d(T0～3)時測定機(jī)械痛閾(MWT)和熱痛閡(TWL),并于T1-3時測定痛閾后處死,取術(shù)側(cè)L4-L6背根神經(jīng)節(jié)(DRG),采用實(shí)時熒光PCR法檢測生長相關(guān)蛋白(GAP-43)mRNA的表達(dá)水平。結(jié)果：與S組比較,NP組和TMP組MWT和TWL在T1-3時降低,背根神經(jīng)節(jié)GAP-43 mRNA表達(dá)上調(diào)(P0.05)。與NP組比較,TMP組MWT和TWL在T1-3時升高,背根神經(jīng)節(jié)GAP-43 mRNA表達(dá)下調(diào)(P0.05)。與T1時比較,NP組和TMP組MWT和TWL在T2-3時升高,背根神經(jīng)節(jié)GAP-43mRNA表達(dá)下調(diào)(P0.05)。結(jié)論：川芎嗪減輕大鼠神經(jīng)病理性疼痛的機(jī)制可能與其抑制脊髓背根神經(jīng)節(jié)GAP-43 mRNA表達(dá)上調(diào)有關(guān)。
[Abstract]:Aim: to evaluate the effect of ligustrazine on the expression of GAP-43 mRNA in spinal dorsal root ganglion (DRG) of rats with chronic sciatic nerve compression injury, and to explore the effect of ligustrazine on neuropathic pain in rats.Methods: 54 adult male healthy Wistar rats were selected.The grouping method is random digital table,54 rats were divided into 3 groups: sham operation group (S group), neuropathic pain group (NP group), tetramethylpyrazine group (TMP group) and TMP group (using chronic sciatic nerve compression injury) to establish neuropathic pain model rats in S group.In the unligated sciatic nerve group, ligustrazine 100mg kg-1 was injected intraperitoneally immediately after CCI.Group S and group NP were intraperitoneally injected with normal saline of equal volume, and 6 rats were randomly selected from group S and group TMP, respectively, on the day of death.The mechanical pain threshold (MWTT) and thermal pain barrier (TWL) were measured 1 day before operation and 7 days after operation, and the pain threshold was killed at T1-3. The expression of growth associated protein (GAP-43) was detected by real-time fluorescence PCR method in the dorsal root ganglion (DRG) of the dorsal root ganglion of L4-L6.Results: compared with S group, MWT and TWL in NP group and TMP group were decreased at T1-3, and GAP-43 mRNA expression was up-regulated in dorsal root ganglion (DRG).Compared with NP group, MWT and TWL in TMP group increased at T1-3 and the expression of GAP-43 mRNA in DRG was down-regulated (P 0.05).Compared with T1, MWT and TWL in NP group and TMP group were increased at T2-3, and the expression of GAP-43mRNA in dorsal root ganglion was down-regulated (P 0.05).Conclusion: the mechanism of ligustrazine in relieving neuropathic pain may be related to the up-regulation of GAP-43 mRNA expression in dorsal root ganglion of spinal cord.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R614

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 黃露露;于世英;;神經(jīng)病理性疼痛的中樞敏化發(fā)病機(jī)制[J];中國疼痛醫(yī)學(xué)雜志;2011年08期

2 張振玉,王崇文,祝金泉,張昆和,謝勇,黃德強(qiáng);川芎嗪及聯(lián)用化療藥物對胃癌細(xì)胞殺傷作用的研究[J];中國現(xiàn)代醫(yī)學(xué)雜志;1999年01期

3 陳輝樂;毛朝鳴;金可可;邱曉曉;方周溪;王萬鐵;徐正yD;;川芎嗪干預(yù)腎缺血再灌注損傷時內(nèi)皮素-1、一氧化氮和超微結(jié)構(gòu)變化[J];中華中醫(yī)藥學(xué)刊;2013年02期

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本文編號：1699361