本文選題：白藜蘆醇　切入點：七氟烷　出處：《重慶醫(yī)科大學》2017年碩士論文

【摘要】：目的:探討白藜蘆醇(Resveratrol,RES)預處理對新生SD大鼠七氟烷麻醉后認知功能改變的影響。方法:將新生SD大鼠隨機分為對照(Con)組,白藜蘆醇(RES)組,七氟烷(Sevo)組,七氟烷+白藜蘆醇(Sevo+RES)組。采用自制的麻醉箱將新生6天(P6)的SD大鼠暴露于2.5%的七氟烷環(huán)境中,持續(xù)3天,每天2 h,建立七氟烷暴露模型,每次七氟烷暴露前30min給予腹腔注射白藜蘆醇(30mg/kg)。對照組與白藜蘆醇組則暴露于除七氟烷外其余條件相同的環(huán)境中。于第3天暴露結束后(P8)立即處死,取腦組織,HE染色觀察腦組織病理形態(tài)學變化;實時熒光聚合酶鏈反應(Real-time polymerase chain reaction,RT-PCR)檢測海馬組織中Sirt1、PGC-1α及FOXO3αm RNA的表達;免疫印跡法(Western blotting,WB)檢測海馬中沉默信息調(diào)節(jié)因子2相關酶I(Silent information adjustment factor 2 related enzyme I,Sirt1)及半胱氨酸天冬氨酸蛋白酶(Cysteiny1 aspartate-specific proteinase,Caspase3)的蛋白表達;超氧化物歧化酶(Superoxide Dismutase,SOD)和丙二醛(Malonaldehyde,MDA)試劑盒化學法檢測海馬組織勻漿中SOD、MDA活性變化;第30天(P30)進行Morris水迷宮實驗評價各組大鼠學習記憶能力。結果:與Con組比較,Sevo組神經(jīng)組織明顯疏松,水腫,部分胞漿溶解,核固縮、碎裂,給予RES預處理七氟烷暴露(Sevo+RES組)后,也可見細胞水腫,核固縮等病理形態(tài),但程度較Sevo組明顯減輕;與Con組比較,Sevo組Sirt1、PGC-1α、FOXO3αm RNA的表達均降低(P0.05),SOD活性降低(P0.05),MDA水平升高(P0.05);而與Sevo組相比,Sevo+RES組Sirt1、PGC-1α及FOXO3αm RNA表達明顯增強(P0.05),SOD活性增高(P0.05),MDA水平降低(P0.05)。Western blot結果顯示Sevo組與Sevo+RES組Sirt1表達均低于Con組(P0.05),Sevo組Caspase3與Con組相比則明顯增加(P0.05);但是與Sevo組相比,Sevo+RES組Sirt1表達升高(P0.05),Caspase3表達降低(P0.05)。Morris水迷宮實驗提示各組間在逃避潛伏期,穿越平臺次數(shù)以及目標象限停留時間上均無明顯差異(P0.05)。結論:白藜蘆醇可以降低氧化應激水平,減輕七氟烷暴露所致新生SD大鼠腦損傷,但是,不論是否給予白藜蘆醇預處理,七氟烷對于SD大鼠的遠期學習記憶能力無明顯損害。
[Abstract]:Objective: to investigate the effect of resveratrol resveratrolone on cognitive function of neonatal SD rats after sevoflurane anesthesia. Methods: neonatal SD rats were randomly divided into control group, resveratrol group and sevoflurane Sevogroup. Sevoflurane resveratrol Sevo res) group. SD rats of 6 days old were exposed to 2.5% sevoflurane for 3 days, 2 hours a day, to establish sevoflurane exposure model. Before each sevoflurane exposure, 30min was given intraperitoneal injection of resveratrol 30 mg / kg. The control group and resveratrol group were exposed to the same conditions except sevoflurane. The histopathologic changes of brain tissue were observed by HE staining and real-time polymerase chain reactionation (RT-PCR) was used to detect the expression of Sirt1, PGC-1 偽 and FOXO3 偽 m RNA in hippocampal tissue. Western blotting assay was used to detect the expression of silencing signal regulating factor-2 related enzymes I(Silent information adjustment factor 2 related enzyme sirt1 and cysteiny1 aspartate-specific protein caspase3 in hippocampus. Superoxide dismutase (SOD) and malondialdehyde (Malonaldehydede MDA) kit were used to detect the activity of MDA in hippocampus homogenate. Morris water maze test was carried out on the 30th day to evaluate the learning and memory ability of the rats in each group. Results: compared with the Con group, the nerve tissue in the Sevo group was obviously loose, edema, partial cytoplasmic dissolution, nuclear pyknosis and fragmentation. After RES was pretreated with sevoflurane in Sevo RES group, cell edema and nuclear pyknosis were also observed, but the degree was much less than that in Sevo group. Compared with Con group, the expression of P0.05 Con 偽 -FOXO 3 偽 m RNA in Sevo group decreased the activity of P0.05, the MDA level increased in Sevo RES group and the expression of FOXO3 偽 m RNA in Sevo RES group was significantly higher than that in Sevo group. The results of Western blot showed that the Sirt1 expression in Sevo group and Sevo RES group was significantly higher than that in Sevo RES group. The results of Western blot showed that the expression of Sirt1 in Sevo RES group was significantly higher than that in Sevo RES group, and the expression of FOXO3 偽 m RNA in Sevo RES group was significantly higher than that in Sevo RES group. The results of Western blot showed that the expression of Sirt1 in Sevo group and Sevo RES group was significantly higher than that in Sevo RES group. Compared with Con group, the expression of Sirt1 in Sevo group was significantly higher than that in Sevo group, but the expression of Caspase3 in Sevo RES group was lower than that in Sevo group. Morris water maze test indicated that the escape latency was increased in each group. Conclusion: resveratrol can reduce oxidative stress level and reduce brain damage induced by sevoflurane exposure in neonatal SD rats. Sevoflurane had no significant damage to the long-term learning and memory ability of SD rats.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R614

【參考文獻】

相關期刊論文 前3條

1 鄭淑文;朱昭瓊;;吸入麻醉藥與學習記憶的關系[J];遵義醫(yī)學院學報;2013年04期

2 郭倩;余音;潘乾廣;石瑤;葉秀峰;;白藜蘆醇保護腦缺血/再灌注損傷與自噬關系的研究[J];中國藥理學通報;2013年07期

3 Ozkan ARIES;Suleyman CAYLI;Eyup ALTINOZ;Iclal GURSES;Neslihan YUCEL;Ayhan KOCAK;Saim YOLOGLU;Yusuf TURKOZ;;Effects of resveratrol and methylprednisolone on biochemical,neurobehavioral and histopathological recovery after experimental spinal cord injury[J];Acta Pharmacologica Sinica;2006年10期

相關博士學位論文 前2條

1 熊萬霞;SIRT1-p53、cAMP/CREB信號通路參與老年大鼠吸入麻醉致學習與記憶損害的機制研究[D];復旦大學;2014年

2 閻文軍;SirT1在高壓氧預處理誘導大鼠腦缺血耐受中的作用機制研究[D];第四軍醫(yī)大學;2011年

相關碩士學位論文 前1條

1 孟然;丁苯酞通過PGC-1α/Nrf2通路發(fā)揮對多發(fā)性硬化小鼠神經(jīng)系統(tǒng)線粒體的保護作用[D];河北醫(yī)科大學;2015年

，

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