本文選題：白藜蘆醇　切入點(diǎn)：七氟烷　出處：《重慶醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探討白藜蘆醇(Resveratrol,RES)預(yù)處理對(duì)新生SD大鼠七氟烷麻醉后認(rèn)知功能改變的影響。方法:將新生SD大鼠隨機(jī)分為對(duì)照(Con)組,白藜蘆醇(RES)組,七氟烷(Sevo)組,七氟烷+白藜蘆醇(Sevo+RES)組。采用自制的麻醉箱將新生6天(P6)的SD大鼠暴露于2.5%的七氟烷環(huán)境中,持續(xù)3天,每天2 h,建立七氟烷暴露模型,每次七氟烷暴露前30min給予腹腔注射白藜蘆醇(30mg/kg)。對(duì)照組與白藜蘆醇組則暴露于除七氟烷外其余條件相同的環(huán)境中。于第3天暴露結(jié)束后(P8)立即處死,取腦組織,HE染色觀察腦組織病理形態(tài)學(xué)變化;實(shí)時(shí)熒光聚合酶鏈反應(yīng)(Real-time polymerase chain reaction,RT-PCR)檢測(cè)海馬組織中Sirt1、PGC-1α及FOXO3αm RNA的表達(dá);免疫印跡法(Western blotting,WB)檢測(cè)海馬中沉默信息調(diào)節(jié)因子2相關(guān)酶I(Silent information adjustment factor 2 related enzyme I,Sirt1)及半胱氨酸天冬氨酸蛋白酶(Cysteiny1 aspartate-specific proteinase,Caspase3)的蛋白表達(dá);超氧化物歧化酶(Superoxide Dismutase,SOD)和丙二醛(Malonaldehyde,MDA)試劑盒化學(xué)法檢測(cè)海馬組織勻漿中SOD、MDA活性變化;第30天(P30)進(jìn)行Morris水迷宮實(shí)驗(yàn)評(píng)價(jià)各組大鼠學(xué)習(xí)記憶能力。結(jié)果:與Con組比較,Sevo組神經(jīng)組織明顯疏松,水腫,部分胞漿溶解,核固縮、碎裂,給予RES預(yù)處理七氟烷暴露(Sevo+RES組)后,也可見細(xì)胞水腫,核固縮等病理形態(tài),但程度較Sevo組明顯減輕;與Con組比較,Sevo組Sirt1、PGC-1α、FOXO3αm RNA的表達(dá)均降低(P0.05),SOD活性降低(P0.05),MDA水平升高(P0.05);而與Sevo組相比,Sevo+RES組Sirt1、PGC-1α及FOXO3αm RNA表達(dá)明顯增強(qiáng)(P0.05),SOD活性增高(P0.05),MDA水平降低(P0.05)。Western blot結(jié)果顯示Sevo組與Sevo+RES組Sirt1表達(dá)均低于Con組(P0.05),Sevo組Caspase3與Con組相比則明顯增加(P0.05);但是與Sevo組相比,Sevo+RES組Sirt1表達(dá)升高(P0.05),Caspase3表達(dá)降低(P0.05)。Morris水迷宮實(shí)驗(yàn)提示各組間在逃避潛伏期,穿越平臺(tái)次數(shù)以及目標(biāo)象限停留時(shí)間上均無(wú)明顯差異(P0.05)。結(jié)論:白藜蘆醇可以降低氧化應(yīng)激水平,減輕七氟烷暴露所致新生SD大鼠腦損傷,但是,不論是否給予白藜蘆醇預(yù)處理,七氟烷對(duì)于SD大鼠的遠(yuǎn)期學(xué)習(xí)記憶能力無(wú)明顯損害。
[Abstract]:Objective: to investigate the effect of resveratrol resveratrolone on cognitive function of neonatal SD rats after sevoflurane anesthesia. Methods: neonatal SD rats were randomly divided into control group, resveratrol group and sevoflurane Sevogroup. Sevoflurane resveratrol Sevo res) group. SD rats of 6 days old were exposed to 2.5% sevoflurane for 3 days, 2 hours a day, to establish sevoflurane exposure model. Before each sevoflurane exposure, 30min was given intraperitoneal injection of resveratrol 30 mg / kg. The control group and resveratrol group were exposed to the same conditions except sevoflurane. The histopathologic changes of brain tissue were observed by HE staining and real-time polymerase chain reactionation (RT-PCR) was used to detect the expression of Sirt1, PGC-1 偽 and FOXO3 偽 m RNA in hippocampal tissue. Western blotting assay was used to detect the expression of silencing signal regulating factor-2 related enzymes I(Silent information adjustment factor 2 related enzyme sirt1 and cysteiny1 aspartate-specific protein caspase3 in hippocampus. Superoxide dismutase (SOD) and malondialdehyde (Malonaldehydede MDA) kit were used to detect the activity of MDA in hippocampus homogenate. Morris water maze test was carried out on the 30th day to evaluate the learning and memory ability of the rats in each group. Results: compared with the Con group, the nerve tissue in the Sevo group was obviously loose, edema, partial cytoplasmic dissolution, nuclear pyknosis and fragmentation. After RES was pretreated with sevoflurane in Sevo RES group, cell edema and nuclear pyknosis were also observed, but the degree was much less than that in Sevo group. Compared with Con group, the expression of P0.05 Con 偽 -FOXO 3 偽 m RNA in Sevo group decreased the activity of P0.05, the MDA level increased in Sevo RES group and the expression of FOXO3 偽 m RNA in Sevo RES group was significantly higher than that in Sevo group. The results of Western blot showed that the Sirt1 expression in Sevo group and Sevo RES group was significantly higher than that in Sevo RES group. The results of Western blot showed that the expression of Sirt1 in Sevo RES group was significantly higher than that in Sevo RES group, and the expression of FOXO3 偽 m RNA in Sevo RES group was significantly higher than that in Sevo RES group. The results of Western blot showed that the expression of Sirt1 in Sevo group and Sevo RES group was significantly higher than that in Sevo RES group. Compared with Con group, the expression of Sirt1 in Sevo group was significantly higher than that in Sevo group, but the expression of Caspase3 in Sevo RES group was lower than that in Sevo group. Morris water maze test indicated that the escape latency was increased in each group. Conclusion: resveratrol can reduce oxidative stress level and reduce brain damage induced by sevoflurane exposure in neonatal SD rats. Sevoflurane had no significant damage to the long-term learning and memory ability of SD rats.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R614

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