本文選題：七氟烷　切入點(diǎn)：高氧性肺損傷　出處：《川北醫(yī)學(xué)院》2017年碩士論文

【摘要】：目的:比較不同濃度七氟烷麻醉對大鼠高氧性肺損傷的影響。方法:1、使用SPSS17.0隨機(jī)數(shù)字發(fā)生器將60只大鼠分為空白對照組(C組,10只)和實(shí)驗(yàn)組(S組,50只)。S組使用5L/min左右的氧氣,濃度維持95%以上,暴露時間48小時,復(fù)制高氧性肺損傷大鼠模型;2、使用SPSS17.0隨機(jī)數(shù)字發(fā)生器將S組的50只大鼠分為5組,分別吸入0%、1%、1.5%、2%、2.5%的七氟烷1小時,并分別命名為S0、S1.0、S1.5、S2.0、S2.5組;3、在完成高氧處理48小時(T1)、七氟烷吸入1小時(T2)兩個時間點(diǎn),經(jīng)10%的水合氯醛麻醉下經(jīng)腹主動脈抽取動脈血、血清及收集左右肺組織;4、動脈血行血?dú)夥治鯬O2、PCO2;使用ELISA法檢測血清TNF-α、IL-8和IL-6濃度;取左肺組織進(jìn)行濕/干(Wet/dry,W/D)比測量;取右肺組織進(jìn)行HE染色觀察病理損傷程度并進(jìn)行評分。評分參考指標(biāo):肺泡腔充血、出血、肺泡腔或血管壁中性粒細(xì)胞浸潤聚集、肺泡壁增厚和透明膜形成等。結(jié)果:1、在高氧處理48小時后(T1),與C組比較,S組的PO2降低、PCO2增加、TNF-α、IL-8和IL-6濃度增加、W/D比值增加,病理損傷評分增加,差異具有顯著性(P0.05);S0至S2.5組組間兩兩比較,上述指標(biāo)均無顯著性差異(P0.05)。2、經(jīng)七氟烷處理1小時后(T2):(1)與C組比較,S組的PO2降低、PCO2增加、TNF-α、IL-8和IL-6濃度增加、W/D比值增加,病理損傷評分增加,差異具有顯著性(P0.05);(2)與七氟烷處理前比較,S1至S2.5組的PO2增加、PCO2降低、TNF-α、IL-8和IL-6濃度降低、W/D比值降低,病理損傷評分降低,差異具有顯著性(P0.05),S0組上述指標(biāo)無顯著性改變(P0.05);(3)與S2.0組比較,S1.0與S1.5組的PO2降低、PCO2增加、TNF-α、IL-8和IL-6濃度增加、W/D比值增加,病理損傷評分增加,差異具有顯著性(P0.05);(4)與S2.0組比較,S2.5組PO2、PCO2、TNF-α、IL-8和IL-6濃度、濕/干比值及病理學(xué)評分無顯著性差異(P0.05)。結(jié)論:1、使用95%濃度氧氣48小時可復(fù)制高氧性肺損傷大鼠模型;2、七氟烷可以有效降低大鼠高氧導(dǎo)致的肺損傷程度;3、2%濃度七氟烷對大鼠高氧性肺損傷有較好的保護(hù)效應(yīng)。
[Abstract]:Objective: to compare the effects of sevoflurane anesthesia at different concentrations on hyperoxia-induced lung injury in rats. Methods: #number0# rats were randomly divided into control group (n = 10) and control group (n = 50) and group S (n = 50). The rat model of hyperoxia lung injury was established by using SPSS17.0 random number generator. The 50 rats in group S were divided into 5 groups and inhaled with 1.5% and 2.5% sevoflurane for 1 hour, respectively. They were named S0 S1.0S1.0S1.5S2.0S2.5 groups. After 48 hours of hyperoxia treatment and 1 hour of sevoflurane inhalation, arterial blood was drawn from the abdominal aorta under 10% chloral hydrate anesthesia at two time points, one hour after hyperoxia treatment, and one hour after inhalation of sevoflurane, and the arterial blood was extracted from the abdominal aorta under 10% chloral hydrate anesthesia. Serum and left and right lung tissues were collected and arterial blood was used to analyze PO2PCO2. Serum TNF- 偽 IL-8 and IL-6 were detected by ELISA method. Wet / dry Wet / dry WR / D ratio was measured in left lung tissue. The right lung tissue was stained with HE to observe the degree of pathological injury and to grade it. The reference indexes were as follows: congestion, hemorrhage, neutrophil infiltration and aggregation in alveolar cavity or vascular wall. Results the alveolar wall thickening and hyaline membrane formation were observed after hyperoxia treatment for 48 hours. Compared with group C, PO2 decreased, PCO2 increased, TNF- 偽 -IL-8 and IL-6 concentration increased, and pathological injury score increased. There was significant difference between group S0 and S2.5. There was no significant difference in the above indexes. After 1 hour of sevoflurane treatment, the PO2 of group S was decreased, and the concentration of TNF- 偽 IL-8 and IL-6 increased, the ratio of TNF- 偽 IL-8 and IL-6 increased, the score of pathological injury increased, compared with group C, the ratio of TNF- 偽 IL-8 and TNF- 偽 IL-8 and the ratio of TNF- 偽 IL-8 and IL-6 increased, and the pathological injury score increased after 1 hour of sevoflurane treatment. Compared with sevoflurane treatment, PO2 increased in S1-S2.5 group and decreased in TNF- 偽 -IL-8 and IL-6 concentration. The ratio of TNF- 偽, IL-8 and IL-6 decreased, and the pathological injury score decreased, compared with that of sevoflurane treatment. There was no significant difference in the above indexes between S0 group and S0 group. Compared with S2.0 group, the decrease of PO2 in S1.0 and S1.5 groups increased the concentration of TNF- 偽 IL-8 and IL-6, and the ratio of TNF- 偽 IL-8 and IL-6 increased, and the pathological injury score increased. The concentrations of IL-8 and IL-6 in group S2.5 were significantly higher than those in group S2.0, and the concentration of IL-8 and TNF- 偽 in group S2.5 were significantly higher than that in group S2.0. There was no significant difference in wet / dry ratio and pathological score (P 0.05). Conclusion: 95% oxygen for 48 hours can induce hyperoxia-induced lung injury in rats. Sevoflurane can effectively reduce the degree of lung injury induced by hyperoxia in rats. Halothane has a better protective effect on hyperoxic lung injury in rats.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R614

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