發(fā)布時(shí)間：2018-03-26 15:32

  本文選題：丙泊酚　切入點(diǎn)：高脂血癥　出處：《中國(guó)現(xiàn)代醫(yī)學(xué)雜志》2017年13期

【摘要】：目的探討亞麻醉、鎮(zhèn)靜及麻醉劑量丙泊酚對(duì)高脂血癥大鼠的空間記憶及海馬組織的影響。方法65只健康成年雄性SD大鼠隨機(jī)分為基礎(chǔ)性喂養(yǎng)組(15只)和高脂飼料喂養(yǎng)組(50只),喂養(yǎng)6周后隨即抽取10只測(cè)定血脂。將復(fù)制模型成功的40只大鼠隨機(jī)分為4組:A組腹腔注射生理鹽水75 mg/kg;B、C、D組分別腹腔注射亞麻醉(10 mg/kg)、鎮(zhèn)靜(30 mg/kg)及麻醉劑量(375 mg/kg)丙泊酚(濃度為1%),連續(xù)給藥5 d,給藥后20 min以及第6~7天均進(jìn)行Morris水迷宮訓(xùn)練,測(cè)定空間記憶能力;給藥后第8天,斷頭取腦,獲得海馬組織,測(cè)定其中Tau蛋白磷酸化水平、Caspase-3表達(dá)水平和γ氨基丁酸(GABA)水平。結(jié)果隨著給藥和訓(xùn)練天數(shù)增加,4組大鼠的逃避潛伏期縮短(P0.05);C組和D組大鼠不同時(shí)間點(diǎn)的逃避潛伏期高于A組和B組(P0.05),且D組高于C組(P0.05);第7天空間搜索實(shí)驗(yàn)時(shí),C組和D組大鼠穿越平臺(tái)次數(shù)低于A組和B組(P0.05),且D組低于C組(P0.05);C組和D組大鼠海馬組織中GABA含量、陽(yáng)性細(xì)胞平均光密度值高于A組和B組(P0.05),且D組高于C組(P0.05)。結(jié)論鎮(zhèn)靜和麻醉劑量的丙泊酚會(huì)降低高脂血癥大鼠的空間記憶能力,可能與海馬組織中GABA的高水平和Tau蛋白過(guò)度磷酸化有關(guān)。
[Abstract]:Objective to study subanesthesia, Effects of sedation and anesthetic dose propofol on spatial memory and hippocampal tissue in hyperlipidemic rats. Methods Sixty-five healthy adult male SD rats were randomly divided into basic feeding group (n = 15) and hyperlipidemic diet group (n = 50). After 6 weeks, 10 rats were randomly divided into 4 groups: group A: normal saline 75 mg / kg intraperitoneal injection of normal saline 75 mg / kg, group D received intraperitoneal injection of subanaesthesia 10 mg / kg, sedation 30 mg / kg) and anesthetic dosage 375 mg / kg) propofol. Morris water maze training was performed 20 min and 6 ~ 7 days after continuous administration for 5 days. On the 8th day after administration, the brain was taken from the head and the hippocampus was obtained. The level of Tau protein phosphorylation and the level of GABA were measured. Results with the increase of drug administration and training days, the escape latency of rats in group C and group D was shortened at different time points. On the 7th day, the frequency of crossing platform in group C and group D was lower than that in group A and group B, and the content of GABA in hippocampus of group D was lower than that of group C and group D. The average optical density of positive cells was higher than that of group A and group B, and that of group D was higher than that of group C. Conclusion sedative and anesthetic doses of propofol can reduce the spatial memory ability of hyperlipidemic rats. It may be related to the high level of GABA and excessive phosphorylation of Tau protein in hippocampus.
【作者單位】： 海南醫(yī)學(xué)院第二附屬醫(yī)院麻醉科;海南醫(yī)學(xué)院第一附屬醫(yī)院解剖教研室;
【分類號(hào)】：R614

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