本文選題：成骨細胞　切入點：皮質(zhì)酮　出處：《中國骨質(zhì)疏松雜志》2017年09期

【摘要】：目的探討皮質(zhì)酮作用下離體SD大鼠成骨細胞SREBP(固醇調(diào)節(jié)元件結(jié)合蛋白,sterol modulates the element binding protein)1、SREBP2、HMGCR(3-羥基-3-甲基戊二酸單酰輔酶A還原酶,3-hydroxy-3-methyl malonate coenzyme A reductase)、FAS(脂肪酸合成酶,fatty acid synthase)的表達改變對細胞內(nèi)脂質(zhì)積聚的影響。方法采用多次膠原酶組織消化法獲得新生SD大鼠顱骨中的成骨細胞作為研究對象,觀察皮質(zhì)酮作用后成骨細胞內(nèi)脂質(zhì)積聚的特征,以及SREBP1、SREBP2、HMGCR、FAS基因及蛋白表達。結(jié)果成骨細胞內(nèi)Nile Red細胞內(nèi)脂質(zhì)染色可見成骨細胞內(nèi)的脂質(zhì)染色隨皮質(zhì)酮濃度的增加的逐漸增多,相同濃度的皮質(zhì)酮在作用的24 h、48 h、72 h后未見明顯差異;細胞內(nèi)SREBP1、SREBP2、HMGCR、FAS基因表達在不同濃度皮質(zhì)酮作用24 h后均明顯增高,48 h、72 h后呈現(xiàn)下降趨勢;細胞內(nèi)SREBP1、SREBP2、HMGCR、FAS蛋白在1μmol/L皮質(zhì)酮作用24 h后表達升高,48 h后與對照組未見明顯差異,72 h后SREBP1、FAS表達降低,SREBP2表達升高,HMGCR未見明顯差異。結(jié)論超生理劑量的皮質(zhì)酮能促進成骨細胞內(nèi)脂質(zhì)異常沉積;不同濃度皮質(zhì)酮作用成骨細胞24 h后能促進細胞內(nèi)SREBP1、SREBP2、HMGCR、FAS基因表達;皮質(zhì)酮作用成骨細胞24 h后促進成骨細胞內(nèi)SREBP1、SREBP2、HMGCR、FAS蛋白的表達。
[Abstract]:Objective to investigate the expression of 3-hydroxy-3-methyl malonate coenzyme A reductase A reductase (3-hydroxy-3-methyl malonate coenzyme A reductase) in osteoblasts of SD rats induced by corticosterone in vitro, and to investigate the expression and modification of fatty acid synthase fatty acid synthase (fatty acid synthase). Methods osteoblasts from the skull of newborn SD rats were obtained by multiple collagenase tissue digestion. To observe the characteristics of lipid accumulation in osteoblasts and the expression of HMGCR-FAS gene and protein in osteoblasts treated with corticosterone, lipid staining in Nile Red cells showed that lipid staining in osteoblasts increased with the increase of corticosterone concentration. There was no significant difference between the same concentration of corticosterone at 24 h, 48 h and 72 h, but the expression of SREBP1 + SREBP2HMGCR-FAS gene in the cells increased significantly after 24 h of treatment with different concentrations of corticosterone, and decreased after 72 h of treatment with different concentrations of corticosterone. The expression of SREBP1HMGCR-FAS protein increased after 24 h treatment with 1 渭 mol/L corticosterone. There was no significant difference in expression of SREBP1FAS between the cells and the control group after treatment with 1 渭 mol/L for 24 h. Conclusion there is no significant difference in the expression of SREBP1FAS after 72 h. Conclusion the hyperphysiological dose of corticosterone can promote osteogenesis. Abnormal lipid deposition in cells; Different concentrations of corticosterone could promote the expression of HMGCR-FAS gene in osteoblasts after 24 h treatment with different concentrations of corticosterone, and promote the expression of FAS protein in osteoblasts with SREBP1- SREBP2- HMGCR-FAS protein after 24 h treatment with corticosterone.
【作者單位】： 廣東醫(yī)科大學附屬醫(yī)院骨科中心;
【基金】：廣東省科技計劃項目(2011B031800172) 湛江市非資助科技攻關(guān)計劃項目(2014B01077)
【分類號】：R68

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本文編號：1665333