本文選題：鈦離子　切入點(diǎn)：鈦顆粒　出處：《南昌大學(xué)》2015年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:構(gòu)建小鼠air-pouch植骨氣囊模型,并選擇鈦顆粒所誘導(dǎo)的骨溶解模型,觀(guān)察在氣囊內(nèi)部注射鈦離子溶液或者鈦顆粒混懸液后對(duì)鈦顆粒所誘導(dǎo)的無(wú)菌性炎癥的程度是否具有一致性,探討鈦離子在炎性因子介導(dǎo)的無(wú)菌性松動(dòng)致炎性過(guò)程中其具有的可能作用。方法:雄性昆明近交系小鼠60只,平均約8-10周齡大小,每只體重約為30g左右。20只小鼠作為氣囊植骨模型顱骨供體。取余下的40只小鼠按照隨機(jī)法分為4組,每組10只。分別設(shè)定為空白對(duì)照組(A組)、鈦顆粒組(B組)、rh-VEGF組(C組)和鈦離子組(D組)。小鼠背部皮膚備皮后皮下注入無(wú)菌空氣3m L作為初始?xì)饽?隔日在氣囊內(nèi)加注1ml無(wú)菌空氣維持,第8天無(wú)菌條件下切開(kāi)氣囊并在內(nèi)壁植入同源顱骨片,完成植骨氣囊模型。植骨后第一天在B、C組小鼠氣囊中注入0.5m L鈦顆粒懸液,D組注入0.5ml鈦離子溶液,A組注入0.5m L生理鹽水溶液。氣囊植骨前第6、7天及植骨后隔天,在C組小鼠氣囊內(nèi)注射0.2m L的rh-VEGF混懸液,A、B、D組則相應(yīng)注射0.2m L的無(wú)菌生理鹽水。植骨2周后使用頸椎脫臼法處死小鼠并取囊壁以及骨片行HE染色觀(guān)察其病理學(xué)改變,免疫組化及q RT-PCR檢測(cè)炎性因子TNF-α、IL-1β、IL-6、VEGF的表達(dá)量改變。結(jié)果:所有小鼠均存活直至實(shí)驗(yàn)完成。大體觀(guān)察發(fā)現(xiàn)A組氣囊紅腫輕,囊壁上新生血管少見(jiàn)。B、C、D組氣囊紅腫明顯加重,可見(jiàn)較多滲出及新生血管,其中C組最重,B組次之,D組介于B、C組之間。組織學(xué)及分子生物學(xué)檢測(cè)表明,B組囊壁炎性反應(yīng)及骨溶解反應(yīng)明顯,囊壁厚度、細(xì)胞密度及TN F-α、IL-1β、IL-6、VEGF表達(dá)量較A組對(duì)照組明顯增加(P0.05);C組囊壁炎性反應(yīng)及骨溶解最為明顯,上述觀(guān)察指標(biāo)均高于B組顆粒組(P0.05);D組囊壁亦可見(jiàn)明顯炎性反應(yīng)及輕微骨溶解,但炎性因子的表達(dá)相較于B組而言則表達(dá)量相當(dāng)或略低(P0.05),較A組則明顯增高(P0.05)。結(jié)論:鈦離子在體內(nèi)可以誘導(dǎo)炎性因子的表達(dá),并且同樣能夠促進(jìn)VEGF的表達(dá)及分泌,效果同加入VEGF促進(jìn)劑后相當(dāng)。抑制鈦離子釋放能夠抑制鈦顆粒所誘導(dǎo)的無(wú)菌性炎性反應(yīng)。
[Abstract]:Objective: to construct the air-pouch mice bone bag model, and select the model of titanium particle induced osteolysis, is observed in the internal air injection of titanium ion solution or titanium particle suspension on titanium particle induced by the inflammatory degree of consistency, to investigate the titanium ion in aseptic loosening of inflammatory cytokines mediated inflammatory process with its possible role. Methods: Kunming male inbred mice 60, an average of about 8-10 weeks of age size, each weighing about 30g.20 mice as a model of skull bone graft balloon donor. The remaining 40 mice randomly divided into 4 groups, 10 rats in each group. Were set as blank control group (A group), titanium particle group (B group), rh-VEGF group (group C) and titanium ion group (D group). The skin of mice skin after subcutaneous injection of sterile air 3M L as the initial balloon, every other day in the airbag with 1ml sterile air to maintain the 8 days under sterile conditions, cut and skull piece in the inner balloon homologous bone graft implantation, complete the airbag model. The first day after bone grafting in B, C group were injected 0.5m L titanium particles air suspension, D group injected 0.5ml titanium ion solution, the A 0.5m group were injected with L saline solution. The bone grafting before balloon day 6,7 and bone graft in C group were injected 0.2m L rh-VEGF air suspension, A, B, D 0.2m L group was injected with sterile saline. Bone graft 2 weeks after mice were killed by cervical dislocation and cystic wall and bone slices stained with HE to observe its change pathological, immunohistochemical and Q RT-PCR detection of inflammatory factor TNF- alpha, IL-1 beta, IL-6, the expression of VEGF. Results: all the mice survived until the end of the experiment. The general observation group A bag red light, the cyst wall neovascularization rare.B, C, D and airbags significantly heavier, more visible exudation and neovascularization, which The most severe in C group, B group, D group is B, C between the groups. Show that the histological and molecular biological detection, B group of cystic wall inflammation and osteolysis, wall thickness, cell density and TN F- alpha, IL-1 beta, IL-6, VEGF expression increased significantly in A group (P0.05); C group of cystic wall inflammation and osteolysis is most obvious, the observation indexes were higher than that of group B particles (P0.05); group D group also showed cystic wall inflammation and mild osteolysis, but the expression of inflammatory cytokines compared with B group, the expression of P0.05 (equivalent to or slightly lower), was significantly higher than A group (P0.05). Conclusion: the expression of titanium ions can induce inflammatory cytokines in vivo, and also can promote the expression of VEGF and secretion, the effect is the same as adding VEGF accelerator after aseptic inflammatory reaction. Inhibition of titanium ions can inhibit the release of titanium particles induced.

【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R687.4

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