本文選題：棕櫚酸　切入點：骨骼肌干細胞　出處：《南京醫(yī)科大學學報(自然科學版)》2017年05期 　論文類型：期刊論文

【摘要】：目的 :觀察棕櫚酸(palmitic acid,PA)對小鼠骨骼肌干細胞分化及功能的影響,為治療衰老相關的肌少癥尋找新思路。方法:對小鼠骨骼肌干細胞誘導分化后,同時予PA(100μmol/L)干預,用熒光染色法、熒光定量PCR測定成肌基因肌球蛋白重鏈(myosin heavy chains,MyHC)表達;用seahorse能量代謝儀檢測細胞的氧耗增加率,熒光定量PCR檢測線粒體融合基因線粒體融合素基因1(mitofusin1,Mfn1)、視神經萎縮癥蛋白基因(opticatrophy 1,Opa1)和裂解基因發(fā)動相關因子基因(dynamin 1-like,Drp1)、線粒體分裂素基因1(fission 1,Fis1)的相對表達量。結果 :PA刺激后,MyHC熒光表達量下降,肌小管融合率減低,同時seahorse能量代謝儀檢測細胞的氧耗增加率(oxygen consumption rate,OCR)降低(P0.05);線粒體融合基因無明顯異常,但裂解基因表達增加,融合/裂解比值下降(P0.05)。結論:游離脂肪酸抑制骨骼肌干細胞分化、影響線粒體氧耗功能,這可能是通過影響線粒體Drp1基因,增加線粒體裂解實現的。
[Abstract]:Objective: To observe the effect of palmitic acid (palmitic, acid, PA) on mice skeletal muscle stem cell differentiation and function, for the treatment of age-related sarcopenia in search of new ideas. Methods: mouse skeletal muscle stem cells after differentiation, and treated with PA (100 mol/L) intervention, using fluorescence staining, fluorescence quantitative determination of PCR muscle myosin heavy chain gene (myosin heavy chains, MyHC) expression; energy metabolism of seahorse cells was assessed with the increase in oxygen consumption rate, fluorescence quantitative PCR detection of mitochondrial fusion gene mitofusin 1 genes (mitofusin1, Mfn1), optic nerve atrophy protein gene (opticatrophy 1, Opa1) and a lytic gene related genes (dynamin, 1-like, Drp1) mitochondrial fission gene 1 (fission 1, Fis1). Results: the relative expression of PA after stimulation with MyHC fluorescence expression decreased, myotubule fusion rate reduce, and energy metabolism of seahorse detected by fine Cellular oxygen consumption rate of increase (oxygen consumption rate, OCR) decreased (P0.05); mitochondrial fusion gene had no obvious abnormalities, but lytic gene expression increased, fusion / cracking ratio decreased (P0.05). Conclusion: free fatty acids inhibit skeletal muscle stem cell differentiation, affecting mitochondrial oxygen consumption function, which may be affected by mitochondria Drp1 gene, increased mitochondrial fission is achieved.

【作者單位】： 南京醫(yī)科大學第一附屬醫(yī)院老年醫(yī)學科;
【基金】：國家自然科學基金面上項目(81370950)
【分類號】：R685

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