本文選題：膝骨關(guān)節(jié)炎　切入點(diǎn)：木瓜蛋白酶　出處：《中國老年學(xué)雜志》2017年09期 　論文類型：期刊論文

【摘要】：目的采取2%木瓜蛋白酶溶液關(guān)節(jié)腔注射和手術(shù)造成關(guān)節(jié)不穩(wěn)兩種方法誘導(dǎo)大鼠膝關(guān)節(jié)骨性關(guān)節(jié)炎(KOA)模型,比較兩種造模方法在不同時(shí)間節(jié)點(diǎn)關(guān)節(jié)軟骨Ⅱ型膠原表達(dá)的異同。方法 54只8周齡SD大鼠按體重分層后隨機(jī)分為3組,每組18只。手術(shù)組手術(shù)切除大鼠右膝內(nèi)側(cè)半月板、切斷內(nèi)側(cè)副韌帶誘導(dǎo)KOA;藥物組通過在大鼠右膝關(guān)節(jié)腔注射2%木瓜蛋白酶溶液誘導(dǎo)KOA;對照組常規(guī)飼養(yǎng)。于造模后第4、6、8周取右膝股骨內(nèi)側(cè)髁關(guān)節(jié)軟骨,行免疫組化染色觀察Ⅱ型膠原表達(dá)。結(jié)果造模后第4、6、8周,手術(shù)組和藥物組軟骨細(xì)胞Ⅱ膠原表達(dá)逐漸減低,軟骨表面缺損,與對照組相比有統(tǒng)計(jì)學(xué)差異(P0.05)。手術(shù)組關(guān)節(jié)軟骨Ⅱ型膠原表達(dá)與藥物組比較無統(tǒng)計(jì)學(xué)差異(P0.05)。結(jié)論手術(shù)切除膝關(guān)節(jié)內(nèi)側(cè)半月板、切斷內(nèi)側(cè)副韌帶和關(guān)節(jié)腔注射木瓜蛋白酶溶液是KOA的理想造模方法,在第4、6、8周關(guān)節(jié)軟骨Ⅱ型膠原表達(dá)逐漸減少,Ⅱ型膠原的表達(dá)手術(shù)組與藥物組比較無統(tǒng)計(jì)學(xué)差異。實(shí)驗(yàn)可根據(jù)個(gè)體需要,做出科學(xué)地選擇。
[Abstract]:Objective to induce the rat model of osteoarthritis of knee joint by injecting 2% papain solution into articular cavity and making joint instability by operation. Methods 54 8-week-old SD rats were randomly divided into 3 groups, 18 rats in each group. The medial meniscus of right knee was resected in the operation group. KOAA was induced by transection of medial collateral ligament; in drug group, 2% papain solution was injected into the right knee joint cavity of rats; in control group, the articular cartilage of medial femoral condyle of right knee was taken at 4 ~ 6 ~ (th) week after modeling. Results the expression of collagen 鈪，

本文編號：1560207