發(fā)布時間：2018-03-01 09:32

  本文關鍵詞： 骨性關節(jié)炎 基質金屬蛋白酶-1 基質金屬蛋白酶-13 白介素-1 關節(jié)牽伸　出處：《山西醫(yī)科大學》2015年碩士論文　論文類型：學位論文

【摘要】：目的:應用Ilizarov環(huán)形外固定對兔膝骨性關節(jié)炎關節(jié)進行不同程度的持續(xù)關節(jié)牽伸,觀察其對關節(jié)軟骨組織形態(tài)學和MMP-1、-13及IL-1β表達的影響,評估治療效果。方法:24只新西蘭大白兔隨機分為正常對照組(A組)、模型對照組(B組)、正常牽伸治療組(C組)及高度牽伸治療組(D組)。模型組及治療組采用關節(jié)腔內注射木瓜蛋白酶溶液的方法建立兔膝骨性關節(jié)炎模型。造模成功后,C、D牽伸治療組手術安裝特制Ilizarov外固定支架,分別進行不同強度牽伸治療,治療時間為6周。實驗結束后觀察各組家兔軟骨組織形態(tài)學變化及軟骨中MMP-1、-13及IL-1β的蛋白表達變化。結果:組織學檢查顯示模型對照組軟骨的病理損害程度明顯重于正常對照組(P0.01),軟骨組織中MMP-1、-13及IL-1β的表達也明顯高于對照組(P0.01)。而經(jīng)關節(jié)牽伸治療后,軟骨的病理損害程度較模型對照組明顯減輕(P0.01),軟骨組織中MMP-1、-13及IL-1β的表達也較模型組顯著減少(P0.01);高度牽伸組中上訴蛋白的表達較正常牽伸組減少(P0.05),但兩組軟骨組織Mankin評分無明顯差異。結論:應用Ilizarov環(huán)形外固定對OA關節(jié)進行持續(xù)穩(wěn)定的關節(jié)牽伸可延緩關節(jié)軟骨病理退變,并可抑制軟骨組織中MMP-1、-13及IL-1β蛋白的表達,其蛋白減少程度可能與牽伸強度有關。
[Abstract]:Objective: to observe the effects of Ilizarov ring external fixation on articular cartilage tissue morphology and the expression of MMP-1- 13 and IL-1 尾 in rabbit knee osteoarthritis. Methods 24 New Zealand white rabbits were randomly divided into three groups: normal control group A, model control group B, normal drafting treatment group C) and high drafting group D group. The model group and the treatment group were treated with articular cavity. The rabbit knee osteoarthritis model was established by injection of papain solution. After the successful establishment of the model, special Ilizarov external fixator was installed in the treatment group. They were treated with different intensities of drafting, The time of treatment was 6 weeks. The changes of cartilage morphology and the expression of MMP-1- 13 and IL-1 尾 in chondrocytes of each group were observed after the experiment. Results: histological examination showed that the pathological damage of cartilage in the model control group was serious. The expression of MMP-1- 13 and IL-1 尾 in chondrocytes was significantly higher than that in control group (P 0.01), and the expression of MMP-1- 13 and IL-1 尾 in cartilage was significantly higher than that in control group (P 0.01). Compared with the model group, the pathological damage of cartilage was significantly alleviated (P 0.01), the expression of MMP-1 + -13 and IL-1 尾 in cartilage tissue was also significantly reduced than that in the model group, and the expression of appellate protein in the high draft group was significantly lower than that in the normal draft group, but the expression of appellate protein in the cartilage tissue in both groups was lower than that in the normal draft group. There was no significant difference in Mankin score. Conclusion: continuous and stable joint drafting with Ilizarov ring external fixation can delay the pathological degeneration of articular cartilage. It also inhibited the expression of MMP-1- 13 and IL-1 尾 in cartilage tissue, and the decrease of MMP-1- 13 and IL-1 尾 protein may be related to the drafting intensity.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R684.3

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