發(fā)布時間：2018-01-27 04:36

  本文關(guān)鍵詞： 神經(jīng)病理性疼痛 脈沖射頻 背根神經(jīng)節(jié) 小膠質(zhì)細胞 IRF8 反義寡核苷酸　出處：《福建醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

【摘要】：【目的】本研究選用結(jié)扎大鼠一側(cè)坐骨神經(jīng)主干制備慢性坐骨神經(jīng)縮窄損傷(CCI)模型,通過一般行為學(xué)、50%機械刺激縮爪反應(yīng)閾值(50%MWT)、熱輻射刺激縮爪反應(yīng)潛伏期(TWL)觀察判斷對大鼠背根神經(jīng)節(jié)(DRG)進行脈沖射頻(PRF)治療及鞘內(nèi)注射干擾素調(diào)節(jié)因子8(IRF8)反義寡核苷酸是否具有緩解疼痛的作用,并通過Western-Blot、免疫組化(IHC)法檢測大鼠脊髓組織中IRF8、離子鈣接合蛋白Iba1表達來闡述脈沖射頻背根神經(jīng)節(jié)緩解疼痛的可能機制�！痉椒ā康谝徊糠置}沖射頻大鼠CCI模型背根神經(jīng)節(jié)對痛閾和脊髓IRF8表達的影響采用大鼠CCI模型,于造模后第7天行背根神經(jīng)節(jié)脈沖射頻治療。造模前,造模后1、3、5、7天,脈沖射頻后1、7、14天,通過觀察一般行為學(xué),50%MWT、TWL檢測大鼠痛覺改變。造模后7天,脈沖射頻后7、14天,Western-Bolt、IHC法檢測IRF8蛋白水平。第二部分鞘內(nèi)注射IRF8反義ODN對大鼠CCI模型痛閾和脊髓IRF8、Iba1表達的影響建立鞘內(nèi)置管和CCI模型,于造模后12h分別進行鞘內(nèi)注射IRF8反義ODN、IRF8錯義ODN、等體積NS,每天1次,共6天。造模前,造模后1、3、5、7、10、12、14天大鼠實施一般行為學(xué)、50%MWT,TWL測定。造模后第7、14天采用Western-Blot法檢測IRF8、Iba1蛋白表達�！窘Y(jié)果】1、與Sham組比,CCI組、CCI+PRF組、CCI+NPRF組大鼠于神經(jīng)損傷后右后爪50%MWT和TWL均顯著降低(P0.01),同時脊髓IRF8蛋白表達上調(diào)(P0.05)。脈沖射頻后1、7、14天CCI+PRF組與治療前比較50%MWT和TWL升高(P0.01),并顯著高于CCI組和CCI+NPRF組(P0.01),但仍低于Sham組(P0.01),脊髓IRF8蛋白的表達則相應(yīng)減少(P0.05)。2、與Sham組比較,NS組、MM組大鼠神經(jīng)損傷后右后爪50%MWT和TWL均顯著降低(P0.01),脊髓IRF8、Iba1蛋白表達則增強(P0.05)。AS組與Sham組相比于CCI后第10天起右后爪50%MWT和TWL下降(P0.01),但仍明顯高于MM組、NS組(P0.01)。CCI后第7、14天IRF8、Iba1蛋白表達,AS組較MM組、NS組顯著減弱(P0.05),但仍強于Sham組(P0.05)。AS組第14天IRF8、Iba1蛋白較第7天增強(P0.05)�！窘Y(jié)論】1、PRF可以減輕CCI模型痛敏,其機制可能是PRF抑制大鼠脊髓IRF8的表達,從而緩解疼痛。2、IRF8是誘導(dǎo)外周神經(jīng)損傷后痛覺敏化的關(guān)鍵因子,提前給予阻斷IRF8可以緩解神經(jīng)病理性疼痛。
[Abstract]:[Objective] this study selected one side of sciatic nerve trunk for ligation of rat chronic constriction injury of the sciatic nerve (CCI) model, the general behavior of 50% mechanical stimulation, paw withdrawal threshold (50%MWT), thermal radiation to stimulate the paw withdrawal latency (TWL) in rat dorsal root ganglion (DRG) were observed pulsed radiofrequency (PRF) therapy and intrathecal injection of interferon regulatory factor 8 (IRF8) or antisense oligonucleotides has the effect of pain relief, and by Western-Blot, immunohistochemistry (IHC) method for the detection of IRF8 in spinal cord of rats, calcium binding protein Iba1 expression to explain the possible mechanism of RF pulse to relieve the pain of dorsal root ganglion [method] the first part. Effects of pulsed radiofrequency CCI model of rat dorsal root ganglion and spinal IRF8 expression on the pain threshold of the rat CCI model was used to model seventh days after dorsal root ganglion pulsed radio frequency treatment. Before modeling, modeling After 1,3,5,7 days, 1,7,14 days after the observation of pulsed radiofrequency, the general behavior, 50%MWT, TWL in rats were detected pain. 7 days after modeling, 7,14 days after pulsed RF Western-Bolt, detection of IRF8 protein level by IHC. The second part of intrathecal injection of IRF8 antisense ODN on CCI rat model of pain threshold and spinal cord IRF8, Iba1 expression and the effects of intrathecal catheter and CCI model, after modeling 12h respectively by intrathecal injection of IRF8 antisense ODN, IRF8 missense ODN, volume NS, 1 times a day, a total of 6 days. Before modeling, after modeling 1,3,5,7,10,12,14 rats in the implementation of the general behavior, 50%MWT, TWL determination of the first 7,14 days after modeling. Western-Blot method was used to detect IRF8 and Iba1 protein expression. [results] 1, compared with the Sham group, CCI group, CCI+PRF group, CCI+NPRF group of rats after nerve injury in the right hind paw of 50%MWT and TWL were significantly decreased (P0.01), while the expression of IRF8 protein in the spinal cord adjustment (P0.05). Pulsed RF 1,7,14 days after CCI +PRF緇勪笌娌葷枟鍓嶆瘮杈，

本文編號：1467586