本文關(guān)鍵詞： 椎間盤退變 基因治療 BMP- 細(xì)胞分化抑制因子　出處：《中國修復(fù)重建外科雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的研究兔腰椎間盤髓核內(nèi)注射慢病毒攜帶的BMP-2和細(xì)胞分化抑制因子1(inhibitor of differentiation 1,Id1)基因能否有效改善椎間盤退變進(jìn)程。方法取4月齡新西蘭兔32只,體質(zhì)量2.0~2.5 kg;以L3、4、L4、5、L5、6為目標(biāo)椎間盤,經(jīng)腹細(xì)針穿刺法制造椎間盤退變模型。將30只造模成功的實驗動物隨機(jī)分為5組,每組6只;造模后4周于各組目標(biāo)椎間盤中分別注射Lv-BMP-2病毒(A組)、Lv-BMP-2+Lv-Id1病毒(B組)、LvId1病毒(C組)、Lv-綠色熒光蛋白空載體病毒(D組)及PBS(E組)。于注射后2、4、8周各組分別隨機(jī)取2只動物行T2-mapping MRI檢查獲得確切的T2弛豫時間(T2值);之后處死動物取椎間盤組織,應(yīng)用實時熒光定量PCR、ELISA法檢測Ⅱ型膠原及蛋白聚糖的m RNA相對表達(dá)量及含量。結(jié)果 T2-mapping MRI檢查示,注射后0、2周各組間T2值比較差異均無統(tǒng)計學(xué)意義(P0.05);注射后4周,A、B組T2值顯著高于C、D、E組(P0.05),A、B組間比較差異無統(tǒng)計學(xué)意義(P0.05);注射后8周,B組T2值顯著高于其余各組(P0.05)。實時熒光定量PCR及ELISA檢測示,注射后2、4、8周B組Ⅱ型膠原及蛋白聚糖的m RNA相對表達(dá)量及含量均顯著高于其余各組(P0.05)。結(jié)論聯(lián)合應(yīng)用BMP-2和Id1基因在體內(nèi)實驗中能有效延緩?fù)醚甸g盤退變的進(jìn)程。
[Abstract]:Objective to study the intramedullary injection of lentivirus-carrying BMP-2 and cell differentiation inhibitor of differentiation 1 into the nucleus pulposus of lumbar intervertebral disc in rabbits. Methods 32 New Zealand rabbits (4 months old) with 2.0 kg body weight and 2.5 kg / kg were used to improve the degenerative process of intervertebral disc. The model of intervertebral disc degeneration was established with L3, L4, L4, L5, L5 and 6 as the target disc. The 30 successful experimental animals were randomly divided into 5 groups, 6 in each group, and 6 in each group were randomly divided into 5 groups, 6 rats in each group, and 6 animals in each group were randomly divided into 5 groups. After 4 weeks of modeling, Lv-BMP-2 virus group A was injected into the target disc of each group, respectively. Group B was injected with Lv-BMP-2 Lv-Id1 virus group B and group C was treated with LvId1 virus. Lv-green fluorescent protein empty vector virus group D) and PBS(E group. After 8 weeks, 2 animals were randomly selected for T2-mapping MRI to obtain the exact T 2 relaxation time and T 2 value. Then the animals were killed to take the intervertebral disc tissue and real-time fluorescence quantitative PCR was used. The relative expression and content of m RNA in type 鈪，

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