本文關(guān)鍵詞：反復(fù)七氟烷暴露對老年大鼠學(xué)習(xí)記憶功能的影響　出處：《南昌大學(xué)》2016年碩士論文　論文類型：學(xué)位論文

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【摘要】：背景術(shù)后認知功能障礙(POCD)是術(shù)后常見的并發(fā)癥之一,嚴重影響患者術(shù)后生活質(zhì)量并增加患者病死率。年齡是已知引起POCD發(fā)生的主要因素之一,但引起POCD的具體機制仍不清楚。目的觀察反復(fù)七氟烷暴露對老年大鼠學(xué)習(xí)記憶功能的影響,以及對海馬區(qū)Aβ和p-tau蛋白表達的影響,探討其對大鼠學(xué)習(xí)記憶影響的可能機制。方法20月齡SD雄性老年大鼠20只,將其隨機分為七氟烷組(S組)及對照組(C組),每組10只。于20月齡時,將S組大鼠暴露于2.1%七氟烷+100%氧氣中2h,對照組只給予氧氣吸入,每周一次。于第四次七氟烷暴露后一周,運用Morris水迷宮系統(tǒng)測定大鼠空間學(xué)習(xí)記憶功能,然后,取大腦海馬組織,通過免疫組化法測定海馬組織CA1區(qū)Aβ和p-tau的分布和表達水平。結(jié)果與C組比較,S組大鼠逃避潛伏期無明顯差異(P0.05),穿越平臺次數(shù)更多但無顯著差異(P0.05),平臺所在象限停留時間更長(P0.05)。與C組比較,S組Aβ、p-tau蛋白和Aβ/p-tau的水平?jīng)]有明顯改變(P0.05)。結(jié)論反復(fù)暴露于2.1%的七氟烷可延緩老年大鼠記憶功能的衰退,對海馬CA1區(qū)Aβ和p-tau蛋白的表達無明顯影響,其機制有待進一步的研究。
[Abstract]:The background of postoperative cognitive dysfunction (POCD) is one of the common complications postoperatively, seriously affect the life quality of patients and increase the mortality of patients. Age is known to cause one of the main factors of POCD, but the specific mechanisms induced by POCD is still unclear. Objective To observe the effect of repeated seven halothane exposure on learning and memory function in aged rats the effect on the expression of A in hippocampus and beta and p-tau protein, and explore the possible mechanism of memory effects on rat study. Methods 20 month old male SD 20 rats were randomly divided into seven sevoflurane group (S group) and control group (C group), 10 rats in each group. At the age of 20 months, S rats were exposed to 2.1% isoflurane seven +100% oxygen 2h, the control group was given oxygen inhalation, fourth times each week. In the seven week after halothane exposure, using Morris water maze learning and memory function of rats were measured, then take the space, the hippocampus group Fabric, through the immune group distribution determination of hippocampus tissue CA1 A beta and p-tau method and the expression level. Results compared with C group, S group rats had no significant difference in escape latency (P0.05), the number of cross platform more but no significant difference (P0.05), platform quadrant longer residence time (P0.05). With the C group, S group, A beta, p-tau did not significantly alter the A protein and beta /p-tau level (P0.05). Conclusion the 2.1% recession repeated exposure to seven isoflurane can delay memory function in aged rats, had no effect on the expression of CA1 in hippocampus region of A beta and p-tau protein, the mechanism need further research.

【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R614

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