多種多樣的聚合物被潛在地廣泛應(yīng)用于組織再生生物材料�？勺⑸涞乃z在生物醫(yī)學領(lǐng)域的應(yīng)用十分廣泛:如細胞支持支架和治療細胞以及各種藥物的輸送系統(tǒng)。這些材料在再生醫(yī)學領(lǐng)域的多項研究中已證明具有理想的性能。先前的研究表明,衍生自天然聚合物的水凝膠具有出色的生物相容性、生物活性、生物降解性和生物利用度。水凝膠能夠借助其3D網(wǎng)絡(luò)結(jié)構(gòu)從而吸收大量的水,這一特性使水凝膠能夠擁有與ECM具有相似特性的基質(zhì),因此它們能夠提供細胞友好的環(huán)境以助其附著,生長和增殖。細胞死亡過程可以自然發(fā)生,也可以由于各種原因在人體內(nèi)發(fā)生。受損的細胞和組織由于失去功能而無法正常地執(zhí)行其功能。自然,如果根本原因是細胞自然死亡,則可以自動修復和恢復失去的功能,而無需藥物干預治療。再生醫(yī)學是現(xiàn)代組織工程中使用的方法之一。研究人員已付出巨大努力,以設(shè)計和開發(fā)能夠支持細胞的支架用于細胞療法。然而,一些報道的水凝膠系統(tǒng)在交聯(lián)后不易通過注射器注射,并且由于其不可移動的性質(zhì)而不能為細胞提供合適的環(huán)境。根據(jù)已有報道,使用精心設(shè)計制備的可注射水凝膠系統(tǒng)不僅可以提供模仿天然人體ECM的環(huán)境,而且可以顯著改善原位組織再生的程度。這項研究的主要內(nèi)容和... 

【文章來源】：中國科學技術(shù)大學安徽省 211工程院校 985工程院校

【文章頁數(shù)】：81 頁

【學位級別】：碩士

【文章目錄】：
摘要
ABSTRACT
CHAPTER 1 GENERAL INTRODUCTION
    1.1 Injectable hydrogels
    1.2 Various methods of developing hydrogels
        1.2.1 Crosslinking based on Michael addition reaction
        1.2.2 Crosslinking based on irradiation
        1.2.3 crosslinking based on Ionic interactions
        1.2.4 In situ crosslinking based on Schiff base reaction
    1.3 Natural and synthetic polymer-based hydrogels
    1.4 Application of hydrogels
        1.4.1 Hydrogels in wound healing and tissue repair
        1.4.2 Hydrogels in tumor therapy
        1.4.3 Cell support
    1.5 Aim of the study
    1.6 Summary of the thesis
    REFERENCES
CHAPTER 2 DESIGN AND VIEWS ON HYDROGELS FOR CELL SUPPORT
    2.1 Background
    2.2 Natural biopolymers
        2.2.1 Sodium alginate biopolymer
        2.2.2 Chitosan
        2.2.3 Hyaluronic acid
        2.2.4 Gelatin
        2.2.5 Chemical modification of natural biopolymers
        2.2.6 Injectable hydrogels for protein delivery
        2.2.7 Current studies on injectable hydrogels as therapeutic delivery systems
        2.2.8 Injectable hydrogel for cell therapy
        2.2.9 Injectable hydrogel formation through Schiff base reaction
    REFERENCES
CHAPTER 3 SYNTHESIS OF OXIDIZED SODIUM ALGINATE BASEDINJECTABLE HYDROGELS
    3.1 Structural units of alginate biopolymer
    3.2 Reagents and instruments
    3.3 Experimental procedures
        3.3.1 Synthesis of aldehyde functionalized alginate (O.A)
        3.3.2 Determination of oxidation degree
        3.3.3 FTIR characterization of oxidized alginate
        3.3.4 Quantification of amino groups of gelatin
        3.3.5 Procedures for injectable hydrogel formation
        3.3.6 Rheological measurement and gelation time
    3.4 Procedures for cell experiment
        3.4.1 Materials
        3.4.2 Hydrogel biocompatibility test
    3.5 Discussion of results
        3.5.1 oxidation of native sodium alginate
        3.5.2 Hydrogel formation
        3.5.3 Rheological measurement and gelation time
        3.5.4 Optimization of hydrogel formation condition
        3.5.5 Hydrogel biocompatibility test
    REFERENCES
CHAPTER 4 CONCLUSION AND FUTURE PERSPECTIVES
ACKNOWLDGEMENTS
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