發(fā)布時間：2019-07-24 09:19

【摘要】：目的·應(yīng)用高壓靜電法結(jié)合冷凍干燥(凍干)法和離子交聯(lián)法制備殼聚糖 明膠多孔微球,探討微球多孔結(jié)構(gòu)形成的影響因素。方法·以三聚磷酸鈉(STPP)為交聯(lián)劑,利用高壓靜電法結(jié)合凍干法和離子交聯(lián)法制備具有多孔結(jié)構(gòu)的殼聚糖多孔微球和殼聚糖 明膠多孔微球,比較殼聚糖 明膠不同體積比、冷凍溫度、飽和STPP的85%乙醇溶液交聯(lián)固化時間對微球多孔結(jié)構(gòu)的影響,光鏡、掃描電鏡、蘇木精-伊紅染色切片觀察殼聚糖多孔微球和殼聚糖 明膠多孔微球的形態(tài)、表面及內(nèi)部結(jié)構(gòu)、粒徑。結(jié)果·高壓靜電結(jié)合凍干法和離子交聯(lián)法制備的微球,球形良好,球體表面及內(nèi)部均有較多微孔,表面與內(nèi)部孔徑相通。飽和STPP的85%乙醇溶液交聯(lián)固化后的殼聚糖 明膠微球結(jié)構(gòu)能在中性溶液中穩(wěn)定存在,冷凍、凍干與交聯(lián)反應(yīng)的先后順序,交聯(lián)時間及二次冷凍溫度影響多孔微球的孔徑。明膠可以和殼聚糖形成聚電解質(zhì)復(fù)合物,也可以作為致孔劑參與多孔結(jié)構(gòu)的形成。結(jié)論·高壓靜電法結(jié)合凍干法和離子交聯(lián)法制備的殼聚糖 明膠多孔微球具有較大的孔徑(100~200μm),適合細胞的生長及遷移。
[Abstract]:Objective To study the influence factors of the porous structure of the microspheres by using the high-voltage electrostatic method in combination with the freeze-drying (freeze-drying) method and the ion cross-linking method to prepare the chitosan-gelatin porous microspheres. The method comprises the following steps: using sodium tripolyphosphate (STPP) as a cross-linking agent, and preparing a chitosan porous microsphere and a chitosan gelatin porous microsphere with a porous structure by using a high-voltage electrostatic method in combination with a freeze-drying method and an ion cross-linking method, and comparing the different volume ratio and the freezing temperature of the chitosan gelatin, The morphology, surface and internal structure and particle size of the porous microspheres and the chitosan-gelatin porous microspheres were observed by light microscope, scanning electron microscope and hematoxylin-eosin staining. Results The microspheres prepared by the method of high-voltage static electricity combined with the freeze-drying method and the ion cross-linking method have good spherical shape, and the surface of the sphere and the inner part of the microsphere have more micropores, and the surface is communicated with the internal pore. The cross-linking time and the secondary freezing temperature influence the pore size of the porous microspheres in the order of the stable existence, the freezing, the freeze-drying and the cross-linking reaction in the neutral solution after the crosslinking and curing of the 85% ethanol solution of the saturated STPP. The gelatin can form a polyelectrolyte complex with the chitosan, and can also be used as a pore-forming agent to participate in the formation of the porous structure. Conclusion The chitosan-gelatin porous microspheres prepared by the combination of freeze-drying and ion-crosslinking have a large pore size (100-200. mu.m), which is suitable for cell growth and migration.
【作者單位】： 上海交通大學醫(yī)學院附屬瑞金醫(yī)院泌尿外科;上海交通大學醫(yī)學院附屬瑞金醫(yī)院普外科;
【基金】：國家高新技術(shù)研究發(fā)展計劃(“863”計劃)(2008AA02Z417) 國家自然科學基金(30672043,31170938)~~
【分類號】：R318.08

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本文編號：2518543