發(fā)布時間：2019-07-01 16:06

【摘要】：研究背景 細菌粘附于材料表面是內(nèi)植物感染的始動環(huán)節(jié)。因此，在材料表面構(gòu)建抗菌涂層阻斷細菌粘附是預(yù)防內(nèi)植物感染的方向性策略。超疏水表面能抑制細菌粘附但不具備殺菌效果。而銀抗菌譜廣、熱穩(wěn)定性好、生物安全性高，但常規(guī)方法制備載銀涂層存在釋放速度快，抗菌有效期短等不足。本研究擬將抗菌性能優(yōu)異的銀引入超疏水表面構(gòu)建，在提高超疏水表面抗菌性能同時抑制載銀突釋，實現(xiàn)持久抗菌目的。 目的 1.探討TiO_2納米管作為納米銀載體釋放規(guī)律及長期抗菌能力；2.探討載銀/TiO_2納米管表面修飾超疏水及抑制細菌粘附-殺滅細菌能力；3.探討超疏水修飾對載銀突釋的抑制作用及釋放規(guī)律影響。 方法 1.電化學(xué)陽極氧化法制備TiO_2納米管載體層,通過修飾氟硅烷(PETS)制備超疏水表面，掃描電鏡（SEM）、X線衍射（XRD）、接觸角儀（CA）等方法分析表面特征及浸潤性，評估超疏水涂層對細菌粘附作用。 2.脈沖電沉積法制備載納米銀/TiO_2納米管涂層，SEM、X射線能譜（XPS）等離子質(zhì)譜儀（ICPMS）等評估表面特征及載銀釋放，并評估抗菌性能及生物相容性。 3.在載納米銀/TiO_2納米管表面修飾PETS制備超疏水涂層，評估對細菌粘附的抑制作用及殺滅粘附細菌能力。通過ICPMS評估超疏水對載銀突釋抑制作用及對釋放規(guī)律影響。通過動物試驗，探討納米銀超疏水涂層的抗菌效能。 結(jié)果 1.電化學(xué)陽極氧化法可制備出排列規(guī)則的TiO_2納米管載體層，管徑80-100nm，管長200-400nm。在其表面修飾PETS制備超疏水涂層，接觸角達168.2°，能明顯抑制細菌粘附。 2.脈沖電沉積法可將納米級銀（10-30nm）均勻分布TiO_2納米管表面及管內(nèi)。而且以5-300、10-300、20-300樣品銀顆粒大小及分布最為均勻，表面含銀比例分別為4.08At%、1.23At%、0.2At%。 3.樣品（5-300、10-300、20-300）銀釋放時間14d，前3天由于表面銀突釋作用釋放量較大，其后呈平穩(wěn)釋放狀態(tài)。載銀樣品周圍均能形成明顯抑菌圈。 4.載銀樣品（5-300、10-300、20-300）具有良好生物相容性，對成骨細胞增殖、分化及礦化能力未見明顯影響，，而且血液相容性良好。 5.在5-300、10-300、20-300樣品表面修飾PETS，其接觸角分別達160.1°、161.6°、158.0°。載銀超疏水表面能有效抑制細菌粘附并殺滅粘附細菌，對載銀突釋具有一定程度的抑制作用，使載銀釋放更加平穩(wěn)。 6.動物實驗表明，相對于TiO_2鈦棒，載銀鈦棒、超疏水載銀鈦棒均能預(yù)防骨髓炎及術(shù)后感染。 結(jié)論 1.脈沖電沉積法可在TiO_2納米管表面及管內(nèi)均勻沉積納米級單質(zhì)銀顆粒。 2.載納米銀/TiO_2納米管涂層能夠持久釋放，體外抗菌效果較好，而且具有良好的生物相容性。 3.載銀超疏水涂層具有抑制細菌粘附-表面抗菌雙重功效。 4.超疏水修飾對載銀突釋具有一定程度的抑制作用，使載銀釋放更加平穩(wěn)。 5.載銀鈦棒、載銀超疏水鈦棒均在一定程度預(yù)防內(nèi)植物術(shù)后感染。
[Abstract]:Study Background The bacterial adhesion to the surface of the material is the starting ring of the internal plant infection. Section. Therefore, the construction of an antibacterial coating on the surface of the material to block bacterial adhesion is a directional measure to prevent the infection of the plant. The super-hydrophobic surface can inhibit the adhesion of bacteria, but does not have the effect of sterilization. the silver antibacterial spectrum is wide, the heat stability is good, the biological safety is high, the silver-carrying coating prepared by the conventional method has the advantages of high release speed, short antibacterial period, and the like In this study, it is proposed to introduce the silver with excellent antibacterial performance into the super-hydrophobic surface, to improve the antibacterial performance of the super-hydrophobic surface and to inhibit the release of the silver-loaded surface, so as to realize the long-lasting antibacterial effect. It's all right. Objective 1. To investigate the release and long-term resistance of TiO _ 2 nanotubes as nano-silver carriers Surface modification of silver-bearing/ TiO _ 2 nanotubes, superhydrophobic and inhibition of bacterial adhesion-killing of fine particles the effect of super-hydrophobic modification on the release of silver-loaded silver and its release to set a gauge Method 1. The preparation of the TiO _ 2 nanotube carrier layer by electrochemical anodic oxidation, the preparation of superhydrophobic surface by modified fluorosilane (PETS), scanning electron microscopy (SEM), X-ray diffraction (XRD), contact angle instrument (CA), etc. Analysis of surface features and wettability, and evaluation of super-hydrophobic The surface features of silver-loaded nano-silver/ TiO _ 2 nanotube coating, SEM, X-ray energy spectrum (XPS) plasma mass spectrometer (ICPMS) were prepared by the pulse electrodeposition method. Preparation of super-hydrophobic coating on the surface modified PETS of nano-silver/ TiO _ 2 nano-tube to evaluate the adhesion of bacteria The inhibitory effect and the ability to kill the adherent bacteria. The super-hydrophobic effect on the silver-bearing process was assessed by the ICPMS. The effect of the release and the effect on the release rule. nanometer Results 1. The electrochemical anodic oxidation method can be used to prepare the TiO2 nanotube carrier layer with regular arrangement, and the tube diameter is 80- 100 nm, the tube length is 200-400 nm. The super-hydrophobic coating and the contact angle are prepared on the surface modified PETS. up to 168.2 擄, the adhesion of bacteria can be obviously inhibited.2. The pulse electrodeposition method can transfer the nano-grade silver (10-30n m) the surface and the tube of the TiO2 nanotube are uniformly distributed, and the size and the distribution of the silver particles in the sample of 5-300,10-300 and 20-300 are the most uniform, the silver-containing ratio of the surface is 4.0, 8At%, 1.23 At%, 0.2 At%.3. Sample (5-300,10-300,20-300) Silver release time 14d, first 3 days due to Table The release amount of the surface silver burst is large, and then it is flat. 4. the silver-carrying sample (5-300,10-300,20-300) has good biocompatibility, 5-300,10-300,20-300 sample surface modified PETS, The antennae reach 160.1 擄, 161.6 擄 and 158.0 擄 respectively. The silver-bearing super-hydrophobic surface can effectively inhibit the adhesion of bacteria and kill the adherent bacteria. 6. The results of animal experiments show that relative to TiO _ 2, the release of silver is more stable. titanium rod Conclusion:1. The pulse of titanium-bearing titanium rod and super-hydrophobic silver-bearing titanium rod can prevent osteomyelitis and post-operative infection. The nano-level elemental silver particles can be uniformly deposited on the surface of the TiO _ 2 nano tube and the tube by the electrodeposition method, and the nano-silver/ TiO _ 2 is loaded. The nanotube coating can be released for a long time, the in vitro antibacterial effect is good, 3. The silver-bearing super-hydrophobic coating has the double work of inhibiting bacterial adhesion-surface antibacterial and 4. the super-hydrophobic modification has a certain inhibition effect on the release of the silver-loaded burst, so that the carrie
【學(xué)位授予單位】：中國人民解放軍醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2013
【分類號】：R318.08;TB383.1

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相關(guān)期刊論文 前1條

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