【摘要】：目的：研究食管上皮細胞在多孔絲素蛋白支架上的粘附和生長情況,觀察人食管上皮細胞與絲素蛋白支架的生物相容性,探索以絲素蛋白支架為基質(zhì)構建組織工程化食管的可行性。 方法：體外分離、培養(yǎng)、擴增人正常食管上皮細胞,并以一定數(shù)量接種于絲素蛋白支架上,在體外持續(xù)培養(yǎng),通過組織學及掃描電鏡觀察食管上皮細胞在體外的增殖與分化情況以及細胞與絲素蛋白支架的生物相容性。 結果：分離培養(yǎng)的人食管上皮細胞呈鋪路石樣,體外可大量擴增,細胞角蛋白抗體免疫組織化學染色陽性,體外培養(yǎng)可見食管上皮細胞在支架上粘附、生長良好,持續(xù)培養(yǎng)仍可保持食管上皮特性。 結論：酶消化法適合食管上皮細胞的分離,所得細胞成分較純,絲素蛋白支架有良好的生物相容性和細胞粘附性,無細胞毒性,適于食管上皮細胞的粘附生長,可作為組織工程食管的支架材料。
[Abstract]:Aim: to study the adhesion and growth of esophageal epithelial cells on porous silk fibroin scaffolds, to observe the biocompatibility of human esophageal epithelial cells with silk fibroin scaffolds, and to explore the feasibility of constructing tissue engineering esophagus with silk fibroin scaffolds. Methods: human normal esophageal epithelial cells were isolated, cultured and expanded in vitro and inoculated on silk fibroin scaffolds. The proliferation and differentiation of esophageal epithelial cells in vitro and the biocompatibility of esophageal epithelial cells with silk fibroin scaffolds were observed by histology and scanning electron microscope. Results: the isolated and cultured human esophageal epithelial cells were paving stone, which could be expanded in vitro, and the cytokeratin antibody was positive. Esophageal epithelial cells adhered to the scaffold in vitro and grew well. Continuous culture could still maintain the characteristics of esophageal epithelial cells. Conclusion: enzyme digestion is suitable for the isolation of esophageal epithelial cells. The obtained cells are pure. Silk fibroin scaffolds have good biocompatibility, cell adhesion and no cytotoxicity. They are suitable for the adhesion and growth of esophageal epithelial cells and can be used as scaffolds for tissue engineering esophagus.
【學位授予單位】：復旦大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R318.08

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