二氧化鈦納米管生物透析膜的通透性和蛋白質(zhì)吸附性能研究
發(fā)布時(shí)間：2019-06-11 01:17
【摘要】：腎臟疾病成為又一個(gè)威脅人類健康的重大疾病，目前世界上有超過5億人口患有不同形式的腎臟疾病，每年有上百萬人死于與其相關(guān)的心腦血管疾病。由于供體太少使腎臟移植治療法受到極大的限制，現(xiàn)在血透或血濾替代治療法成為了腎臟失去功能后一種成功的維持性替代治療方法。因此，研究生物透析膜對(duì)于進(jìn)行血液透析的腎臟治療意義重大。 采用陽極氧化法制備的二氧化鈦納米管陣列，具有良好的形貌，進(jìn)行腐蝕以后，可以得到兩端通透的納米管陣列。得到的通透的納米管陣列可以應(yīng)用于生物過濾和生物透析領(lǐng)域。 本論文首先研究了二氧化鈦納米管陣列的制備工藝，分別采用兩步陽極氧化法和化學(xué)腐蝕法來消除納米管表面聚集的“納米線”，制備出了形貌良好、無“納米線”的納米管陣列。通過控制陽極氧化電壓參數(shù)得到了不同管徑的納米管陣列。同時(shí)采用揮發(fā)的氫氟酸氣體對(duì)剝落的二氧化鈦納米膜封閉的底部進(jìn)行刻蝕，獲得兩端通透的納米管陣列。實(shí)驗(yàn)表明，對(duì)比化學(xué)腐蝕法，兩步陽極氧化法制備的二氧化鈦納米管陣列形貌更好更穩(wěn)定；用揮發(fā)的HF氣體對(duì)納米管陣列底部刻蝕，獲得通透納米管陣列的最佳刻蝕時(shí)間為15min。 研究了苯酚紅、牛血清白蛋白（BSA）在二氧化鈦納米膜上的通透性能以及BSA在二氧化鈦納米膜上的靜態(tài)吸附和動(dòng)態(tài)吸附過程。采用光電比色計(jì)和紫外分光光度計(jì)進(jìn)行苯酚紅和BSA的通透研究，探討了納米管管徑形貌參數(shù)對(duì)納米膜通透性能的影響規(guī)律。發(fā)現(xiàn)管徑越大，通透性能越好，且苯酚紅的通透性能要優(yōu)于BSA。采用熒光標(biāo)記法、紅外光譜法（FTIR）、X射線光電子能譜分析（XPS），對(duì)比研究了不同管徑二氧化鈦納米透析膜以及幾種高分子透析膜的蛋白質(zhì)靜態(tài)吸附和動(dòng)態(tài)吸附規(guī)律。結(jié)果表明，蛋白質(zhì)的吸附量與膜的種類、吸附時(shí)間、二氧化鈦納米管管徑和蛋白質(zhì)溶液流速有關(guān)。在考察的各種透析膜中，其蛋白質(zhì)靜態(tài)吸附的最終吸附量較為一致，一般在8—9μg/mm~2左右。而膜的種類不同會(huì)影響到蛋白質(zhì)動(dòng)態(tài)吸附的最終吸附量；在動(dòng)態(tài)條件下蛋白質(zhì)在二氧化鈦納米膜上的吸附要小于在高分子膜上的吸附，其最終吸附量分別為6—7μg/mm~2和7—8μg/mm~2。在動(dòng)態(tài)吸附條件下，蛋白質(zhì)溶液流量為5.5ml/min時(shí)的蛋白質(zhì)吸附量要大于流量為2.5ml/min和8ml/min的蛋白質(zhì)吸附量。 設(shè)計(jì)并制造了一種新型透析器芯片。采用研制的TiO_2納米管生物透析膜，并與再生纖維素膜、混合纖維素膜和聚醚砜（PES）膜作對(duì)比，分別對(duì)含有維生素B12、尿素、磷酸鹽的代血漿進(jìn)行了透析研究。同時(shí)在透析器芯片中通入蛋白質(zhì)溶液，研究蛋白質(zhì)對(duì)透析裝置的影響規(guī)律。結(jié)果表明，TiO_2納米管生物透析膜的透析性能要優(yōu)于高分子透析膜，在三種高分子透析膜中混合纖維素膜和PES膜的透析性能要優(yōu)于再生纖維素膜；對(duì)于各種透析膜而言，均是維生素B12的清除率最小，對(duì)于再生纖維素膜而言，磷酸鹽的清除率較大，，對(duì)于其他三種透析膜，尿素的清除率最大。整個(gè)的透析過程中，蛋白質(zhì)的流失率小于16%，而且隨時(shí)間的延長，流失率變小，基本趨于平衡。雖然在透析膜材料表面會(huì)有蛋白質(zhì)的吸附，但是吸附的蛋白質(zhì)很少，而且膜表面的大部分孔隙都未被堵塞，蛋白質(zhì)的吸附不會(huì)影響透析器的功能。證實(shí)該新型透析器芯片可以應(yīng)用于血液透析替代人體腎臟過濾功能，為可便攜式或可移植式的生物人工腎的研制打下了良好的實(shí)驗(yàn)基礎(chǔ)。
[Abstract]:Kidney disease is another major disease that threatens human health. There are more than 500 million people in the world with different forms of kidney disease, and millions of people die each year from their associated cardiovascular and cerebrovascular diseases. Since the donor is too small to limit the renal transplantation therapy, the hemodialysis or hemofiltration replacement therapy has become a successful maintenance alternative to the loss of kidney function. Therefore, the study of the biological dialysis membrane is of great significance in the treatment of the kidney for hemodialysis. The titanium dioxide nanotube array prepared by the method of the anode oxidation has good morphology and can be used for obtaining two-end transparent nanotube array after the corrosion is carried out. Column. The resulting permeable nanotube array can be applied to both biofiltration and biodialysis The preparation process of the titanium dioxide nanotube array is first studied in this paper. The "nano-wire" of the surface aggregation of the nanotubes is eliminated by the two-step anodic oxidation method and the chemical corrosion method, and the nano-TiO2 nanotube array has good morphology and no "nano-wire". The tube array is obtained by controlling the parameters of the anodic oxidation voltage to obtain the nanometer tube with different pipe diameters. and simultaneously, a volatile hydrofluoric acid gas is used for etching the bottom of the stripped titanium dioxide nano-film, so as to obtain the transparent nano-film at the two ends The experiment shows that the morphology of the titanium dioxide nanotube array prepared by the two-step anodic oxidation method is better and more stable compared with the chemical corrosion method, the bottom of the nanotube array is etched with the volatile HF gas, and the optimal etching time of the transparent nanotube array is 15. The permeability of bovine serum albumin (BSA) on the titanium dioxide nanomembrane and the static adsorption of BSA on the titanium dioxide nanomembrane were studied. The pass-through study of phenol red and BSA was carried out by a photoelectric colorimeter and an ultraviolet spectrophotometer, and the permeability of the nano-membrane was discussed. It is found that the larger the pipe diameter, the better the permeability, and the permeability of the phenol red Compared with BSA, the static adsorption and dynamic properties of the titanium dioxide nano-dialysis membrane and several high-molecular dialysis membranes were studied by means of fluorescence labeling, infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The results show that the adsorption amount of the protein is the same as that of the membrane, the adsorption time, the diameter of the titanium dioxide nanotube and the dissolution of the protein. The final adsorption capacity of the protein in the various dialysis membranes is more consistent, generally at 8-9 & mu; g/ m And the adsorption of the protein on the titanium dioxide nano-membrane under the dynamic condition is less than that on the high-molecular membrane, and the final adsorption amount of the protein is 6-7 & mu; g/ mm-2 and 7-8 & mu; g, respectively. The protein adsorption capacity of the protein solution at 5.5 ml/ min under dynamic adsorption is more than 2.5ml/ min and 8ml/ min. White matter adsorption. A design and manufacture of a white matter. A new type of dialyser chip was made by using the prepared TiO _ 2 nanotube biological dialysis membrane and comparing with the regenerated cellulose membrane, the mixed cellulose membrane and the polyetheric membrane (PES) membrane, respectively, the generation of plasma containing the vitamin B12, the urea and the phosphate Dialysis was carried out. At the same time, the protein solution was introduced into the dialyser chip to study the effect of protein on dialysis. The results show that the dialysis performance of the TiO _ 2 nanotube bio-dialysis membrane is superior to that of the high-molecular dialysis membrane, and the dialysis performance of the mixed cellulose membrane and the PES membrane in the three high-molecular dialysis membranes is superior to that of the regenerated cellulose membrane; for various dialysis membranes, it is the vitamin B1 2 has the smallest clearance and, for the regenerated cellulose membrane, the clearance of the phosphate is large, for the other three dialysis membranes, urine, In the whole dialysis process, the flow rate of the protein is less than 16%, and the loss rate decreases with the extension of time. And the adsorption of the protein is little, and most of the pores on the surface of the membrane are not blocked, and the adsorption of the protein is not reflected. In response to the function of the dialyzer, it is confirmed that the new type of dialyser chip can be used for hemodialysis instead of the human kidney filtering function, and lays a foundation for the development of a portable or portable biological artificial kidney.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08
本文編號(hào)：2496887
[Abstract]:Kidney disease is another major disease that threatens human health. There are more than 500 million people in the world with different forms of kidney disease, and millions of people die each year from their associated cardiovascular and cerebrovascular diseases. Since the donor is too small to limit the renal transplantation therapy, the hemodialysis or hemofiltration replacement therapy has become a successful maintenance alternative to the loss of kidney function. Therefore, the study of the biological dialysis membrane is of great significance in the treatment of the kidney for hemodialysis. The titanium dioxide nanotube array prepared by the method of the anode oxidation has good morphology and can be used for obtaining two-end transparent nanotube array after the corrosion is carried out. Column. The resulting permeable nanotube array can be applied to both biofiltration and biodialysis The preparation process of the titanium dioxide nanotube array is first studied in this paper. The "nano-wire" of the surface aggregation of the nanotubes is eliminated by the two-step anodic oxidation method and the chemical corrosion method, and the nano-TiO2 nanotube array has good morphology and no "nano-wire". The tube array is obtained by controlling the parameters of the anodic oxidation voltage to obtain the nanometer tube with different pipe diameters. and simultaneously, a volatile hydrofluoric acid gas is used for etching the bottom of the stripped titanium dioxide nano-film, so as to obtain the transparent nano-film at the two ends The experiment shows that the morphology of the titanium dioxide nanotube array prepared by the two-step anodic oxidation method is better and more stable compared with the chemical corrosion method, the bottom of the nanotube array is etched with the volatile HF gas, and the optimal etching time of the transparent nanotube array is 15. The permeability of bovine serum albumin (BSA) on the titanium dioxide nanomembrane and the static adsorption of BSA on the titanium dioxide nanomembrane were studied. The pass-through study of phenol red and BSA was carried out by a photoelectric colorimeter and an ultraviolet spectrophotometer, and the permeability of the nano-membrane was discussed. It is found that the larger the pipe diameter, the better the permeability, and the permeability of the phenol red Compared with BSA, the static adsorption and dynamic properties of the titanium dioxide nano-dialysis membrane and several high-molecular dialysis membranes were studied by means of fluorescence labeling, infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The results show that the adsorption amount of the protein is the same as that of the membrane, the adsorption time, the diameter of the titanium dioxide nanotube and the dissolution of the protein. The final adsorption capacity of the protein in the various dialysis membranes is more consistent, generally at 8-9 & mu; g/ m And the adsorption of the protein on the titanium dioxide nano-membrane under the dynamic condition is less than that on the high-molecular membrane, and the final adsorption amount of the protein is 6-7 & mu; g/ mm-2 and 7-8 & mu; g, respectively. The protein adsorption capacity of the protein solution at 5.5 ml/ min under dynamic adsorption is more than 2.5ml/ min and 8ml/ min. White matter adsorption. A design and manufacture of a white matter. A new type of dialyser chip was made by using the prepared TiO _ 2 nanotube biological dialysis membrane and comparing with the regenerated cellulose membrane, the mixed cellulose membrane and the polyetheric membrane (PES) membrane, respectively, the generation of plasma containing the vitamin B12, the urea and the phosphate Dialysis was carried out. At the same time, the protein solution was introduced into the dialyser chip to study the effect of protein on dialysis. The results show that the dialysis performance of the TiO _ 2 nanotube bio-dialysis membrane is superior to that of the high-molecular dialysis membrane, and the dialysis performance of the mixed cellulose membrane and the PES membrane in the three high-molecular dialysis membranes is superior to that of the regenerated cellulose membrane; for various dialysis membranes, it is the vitamin B1 2 has the smallest clearance and, for the regenerated cellulose membrane, the clearance of the phosphate is large, for the other three dialysis membranes, urine, In the whole dialysis process, the flow rate of the protein is less than 16%, and the loss rate decreases with the extension of time. And the adsorption of the protein is little, and most of the pores on the surface of the membrane are not blocked, and the adsorption of the protein is not reflected. In response to the function of the dialyzer, it is confirmed that the new type of dialyser chip can be used for hemodialysis instead of the human kidney filtering function, and lays a foundation for the development of a portable or portable biological artificial kidney.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08
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