【摘要】：體外檢測(cè)草魚魚皮酸溶性膠原蛋白(FASC)的細(xì)胞相容性。采用MTT實(shí)驗(yàn),檢測(cè)不同濃度的FASC溶液或FASC涂層膠對(duì)NIH3T3成纖維細(xì)胞增殖能力的影響(n=6);利用transwell趨化實(shí)驗(yàn),檢測(cè)不同濃度的FASC溶液對(duì)成纖維細(xì)胞的趨化作用(n=3);利用transwell侵襲實(shí)驗(yàn),檢測(cè)不同濃度FASC涂層膠的細(xì)胞通透性(n=3)。結(jié)果表明,FASC溶液濃度依賴性地促進(jìn)NIH3T3成纖維細(xì)胞增殖,16μg/mL的FASC溶液在48和96 h的增殖率分別為63.7%±7.9%和87.3%±8.7%,較對(duì)照組有顯著性差異(P0.001)。NIH3T3細(xì)胞能夠在FASC涂層膠上生長(zhǎng),各實(shí)驗(yàn)組與對(duì)照組相比,增殖率均無(wú)顯著性差異(P0.05)。趨化實(shí)驗(yàn)顯示,FASC溶液各濃度梯度組與陰性對(duì)照組的趨化指數(shù)均有顯著性差異(P0.05),提示FASC對(duì)成纖維細(xì)胞具有趨化作用。侵襲實(shí)驗(yàn)結(jié)果顯示,成纖維細(xì)胞可以通過(guò)FASC涂層膠。實(shí)驗(yàn)證實(shí),FASC與NIH3T3細(xì)胞具有良好的相容性。
[Abstract]:The cytotoxicity of acid-soluble collagen (FASC) in grass carp skin was detected in vitro. MTT assay was used to detect the effect of different concentrations of FASC solution or FASC coating gel on the proliferation of NIH3T3 fibroblasts (n 鈮，

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