【摘要】：背景:合金化可提高純鎂的抗腐蝕性能,延緩其降解速率;大量文獻(xiàn)表明鋅具有良好的抗腫瘤效果。目的:評(píng)價(jià)含鋅鎂合金對人成骨肉瘤U2OS細(xì)胞體外增殖及凋亡的影響。方法:檢測純鎂及不同含鋅量鎂合金(質(zhì)量分?jǐn)?shù)2%、4%、6%)在Hank’s液中的氫氣釋放速率。取人成骨肉瘤U2OS細(xì)胞(或MC3T3-E1細(xì)胞),分別與不同含鋅量鎂合金浸提液、純鎂浸提液及鈦合金浸提液共培養(yǎng)1,3,5 d,MTT法檢測細(xì)胞增殖。取人成骨肉瘤U2OS細(xì)胞,分別與不同含鋅量鎂合金浸提液、純鎂浸提液及鈦合金浸提液共培養(yǎng)24 h,流式細(xì)胞儀分析細(xì)胞凋亡,Western Blotting檢測凋亡相關(guān)蛋白的表達(dá)水平。結(jié)果與結(jié)論:①在初始的100 h內(nèi),純鎂組氫氣釋放速率明顯高于含鋅鎂合金組;在不同含鋅量鎂合金組中,以含鋅量4%鎂合金組氫氣釋放速率最慢;②不同含鋅量鎂合金均可明顯抑制人成骨肉瘤U2OS細(xì)胞的增殖,且與鋅含量呈正相關(guān);不同含鋅量鎂合金對MC3T3-E1細(xì)胞的毒性在0至1級(jí)之間;③不同含鋅量的鎂合金可明顯促進(jìn)人成骨肉瘤U2OS細(xì)胞的凋亡,且與鋅含量呈正相關(guān);④不同含鋅量鎂合金可上調(diào)人成骨肉瘤U2OS細(xì)胞p53、Bax蛋白表達(dá),下調(diào)Bcl-2蛋白表達(dá),改變Bcl-2/Bax比例,其中以含鋅量4%鎂合金效果最顯著;⑤結(jié)果表明:含鋅鎂合金可抑制人成骨肉瘤U2OS細(xì)胞的體外增殖,誘導(dǎo)其凋亡。
[Abstract]:Background: alloying can improve the corrosion resistance and delay the degradation rate of pure magnesium, and a large number of literatures show that zinc has good antitumor effect. Aim: to evaluate the effect of zinc magnesium alloy on proliferation and apoptosis of human osteosarcoma U2OS cells in vitro. Methods: the hydrogen release rate of pure magnesium and magnesium alloys with different zinc content (mass fraction 2%, 4%, 6%) in Hank' s solution was measured. Human osteosarcoma U2OS cells (or MC3T3-E1 cells) were co-cultured with magnesium alloy extract with different zinc content, pure magnesium extract and titanium alloy extract for 1 day and 5 days, respectively. the proliferation of human osteosarcoma cells was detected by MC3T3-E1 assay. Human osteosarcoma U2OS cells were co-cultured with magnesium alloy extract with different zinc content, pure magnesium extract and titanium alloy extract for 24 H. the expression of apoptosis-related proteins was detected by flow cytometry., Western Blotting was used to detect the expression of apoptosis-related proteins. Results and conclusion: 1 the hydrogen release rate of pure magnesium group was significantly higher than that of zinc magnesium alloy group within the initial 100 h, and the hydrogen release rate of zinc content 4% magnesium alloy group was the slowest in different zinc content magnesium alloy group, and the hydrogen release rate of pure magnesium alloy group was significantly higher than that of zinc magnesium alloy group, and the hydrogen release rate of zinc content 4% magnesium alloy group was the slowest. (2) magnesium alloys with different zinc content could significantly inhibit the proliferation of human osteosarcoma U2OS cells, which were positively correlated with zinc content, and the toxicity of magnesium alloys with different zinc content to MC3T3-E1 cells was between 0 and 1 grade, and the toxicity of magnesium alloys with different zinc content to MC3T3-E1 cells was between 0 and 1, and the toxicity of magnesium alloys with different zinc content to MC3T3-E1 cells was between 0 and 1. (3) magnesium alloys with different zinc content could significantly promote the apoptosis of human osteosarcoma U2OS cells, which was positively correlated with zinc content. (4) magnesium alloy with different zinc content could up-regulate the expression of p53, down-regulate the expression of Bcl-2 protein and change the proportion of Bcl-2/Bax in human osteosarcoma U2OS cells, among which 4% magnesium alloy had the most significant effect. The results showed that zinc magnesium alloy could inhibit the proliferation and induce apoptosis of human osteosarcoma U2OS cells in vitro.
【作者單位】： 煤炭總醫(yī)院骨科;
【分類號(hào)】：R318.08;R738.1

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