發(fā)布時(shí)間：2019-03-22 20:28

【摘要】：神經(jīng)損傷后可植入性組/細(xì)胞支架設(shè)計(jì)是周圍神經(jīng)損傷、和脊髓損傷后神經(jīng)修復(fù)一個(gè)重要思路。本研究利用靜電紡絲技術(shù)成功構(gòu)建具有隨或有序拓?fù)浣Y(jié)構(gòu)聚甲丙烯酸甲酯（PMMA）納米纖維材料，并進(jìn)行大鼠原代背根神經(jīng)節(jié)神經(jīng)元（DRGn）培養(yǎng)，及其與大鼠原代雪旺細(xì)胞（SCs）共培養(yǎng)。結(jié)果顯示所構(gòu)建PMMA電紡納米纖維具有良好生物相容性，適合進(jìn)行神經(jīng)細(xì)胞培養(yǎng)；通過所轉(zhuǎn)染綠色熒光蛋白表達(dá)，發(fā)現(xiàn)DRGn能夠順利在電紡纖維上貼壁并生長，電紡纖維拓?fù)浣Y(jié)構(gòu)能夠影響DRGn生長能力和生長方向，DRGn在有序電紡納米纖維上能夠生成更長神經(jīng)突，且神經(jīng)突生長方向能夠和及質(zhì)纖維延伸方向保持高度一致；通過進(jìn)一步分析及掃描電鏡檢查，發(fā)現(xiàn)不管在隨或有序PMMA電紡納米纖維，DRGn神經(jīng)突均能夠沿纖維行，，但相比隨纖維，有序電紡纖維對(duì)DRGn引導(dǎo)能力更強(qiáng)，DRGn似乎更傾向于循有序纖維生長；通過DRGn與SCs共培養(yǎng)，則發(fā)現(xiàn)較PMMA薄膜和隨電紡纖維，有序電紡纖維能夠在形態(tài)上促成DRGn與SCs建立更多共定位，估計(jì)能夠促進(jìn)后續(xù)髓鞘化進(jìn)程。本課研究結(jié)果示有序PMMA電紡納米纖維具有作為神經(jīng)損傷后植入性支架力，值后續(xù)更進(jìn)一步體內(nèi)研究。
[Abstract]:The design of implantable group / cell scaffold after nerve injury is an important idea for peripheral nerve injury and nerve repair after spinal cord injury. In this study, poly (methyl methacrylate) (PMMA) nanofibers with random or ordered topological structure were successfully constructed by electrospinning technique, and the primary dorsal root ganglion neurons (DRG) of rats were cultured with (DRGn). And co-cultured with rat primary Schwann cells (SCs). The results showed that the PMMA electrospun nanofibers had good biocompatibility and were suitable for nerve cell culture. Through the expression of green fluorescent protein, it was found that DRGn could attach and grow on electrospun fibers smoothly. The topological structure of electrospun fibers could affect the growth ability and growth direction of DRGn, and DRGn could produce longer nerve processes on ordered electrospun nanofibers. And the direction of neurite growth was highly consistent with the direction of fiber extension. By means of further analysis and scanning electron microscopy, it was found that the DRGn nerve processes were able to walk along the fibers, regardless of whether they were electrospun with or without PMMA, but the ordered electrospun fibers had stronger ability to guide DRGn than with fibers. DRGn seems to be more inclined to follow the order of fiber growth; By co-culture of DRGn and SCs, it was found that the ordered electrospun fibers could promote more co-localization of DRGn and SCs in morphology than those of PMMA thin films and electrospun fibers, and could promote the subsequent myelinization process. The results of this study show that ordered PMMA electrospun nanofibers have implantable scaffold force after nerve injury and further study in vivo.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2014
【分類號(hào)】：R651.3;R318.08

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李樹峰;王正敏;李雯;;用于神經(jīng)突導(dǎo)向研究的螺旋神經(jīng)元培養(yǎng)體系初步觀察[J];中國眼耳鼻喉科雜志;2011年02期

2 李深;秦華民;藍(lán)曉艷;王蘇平;;神經(jīng)細(xì)胞粘附分子通過P21活化激酶1促進(jìn)神經(jīng)突生長[J];中國組織化學(xué)與細(xì)胞化學(xué)雜志;2013年05期

3 夏海堅(jiān);劉丹;鐘東;晏怡;夏永智;唐文淵;孫曉川;;聚甲基丙烯酸甲酯靜電紡絲納米纖維的拓?fù)浣Y(jié)構(gòu)對(duì)于PC12細(xì)胞神經(jīng)突生長能力和方向的影響[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2014年04期

4 王豐;朱悅;;Nogo-66抑制神經(jīng)元神經(jīng)突生長的研究[J];中國現(xiàn)代醫(yī)學(xué)雜志;2007年18期

5 張擁軍;Rho GTP酶與智力落后[J];國外醫(yī)學(xué)(兒科學(xué)分冊(cè));2004年03期

6 沈劍虹 ,羅其中;中樞神經(jīng)再生抑制信號(hào)傳導(dǎo)機(jī)制研究進(jìn)展[J];國際神經(jīng)病學(xué)神經(jīng)外科學(xué)雜志;2005年04期

7 ;[J];;年期

8 ;[J];;年期

9 ;[J];;年期

10 ;[J];;年期

相關(guān)會(huì)議論文 前1條

1 范麗菲;莫日根;;小G蛋白R(shí)if與神經(jīng)突發(fā)育的研究[A];細(xì)胞—生命的基礎(chǔ)——中國細(xì)胞生物學(xué)學(xué)會(huì)2013年全國學(xué)術(shù)大會(huì)·武漢論文摘要集[C];2013年

相關(guān)重要報(bào)紙文章 前1條

1 馬金林;補(bǔ)藥能提高孩子智力嗎[N];中國中醫(yī)藥報(bào);2007年

相關(guān)博士學(xué)位論文 前3條

1 馬驥;Galanin在神經(jīng)細(xì)胞發(fā)育過程中的作用研究[D];華東師范大學(xué);2008年

2 李樹峰;電場導(dǎo)向螺旋神經(jīng)元神經(jīng)突生長的體外實(shí)驗(yàn)研究[D];復(fù)旦大學(xué);2008年

3 夏海堅(jiān);有序排列PMMA電紡納米纖維在引導(dǎo)大鼠背根神經(jīng)元生長中的價(jià)值探討[D];重慶醫(yī)科大學(xué);2014年

相關(guān)碩士學(xué)位論文 前1條

1 楊明;MeCP2在神經(jīng)發(fā)育中的作用及其分子機(jī)制[D];北京協(xié)和醫(yī)學(xué)院;2011年

本文編號(hào)：2445894