發(fā)布時間：2019-02-23 11:01

【摘要】：目的采用超聲輔助酶法去抗原處理異種骨,期望獲得具有免疫原性低、具有良好生物力學(xué)性能的理想骨移植材料。方法將豬異種骨用超臨界CO2脫脂、深低溫冷凍處理后分為3組,分別采用超聲波輔助胰蛋白酶法去抗原(A組)、單純采用超聲波法(B組)或胰蛋白酶法(C組)處理異種骨,并取新鮮骨為D組。觀察和比較各組的骨材料大體標(biāo)本和切片、抗原性、材料的生物力學(xué)特性。結(jié)果超聲輔助酶法去抗原處理異種骨速度快,經(jīng)處理后的豬異種骨材料去抗原更徹底,免疫原性低,孔隙率最高,孔徑大小最佳,但材料的生物力學(xué)性能有明顯下降。結(jié)論超聲輔助酶法是一種較好的骨材料去抗原處理方法,但處理完后對材料的生物力學(xué)性能有明顯影響,還需要進(jìn)一步優(yōu)化實驗參數(shù)。
[Abstract]:Objective to obtain an ideal bone graft material with low immunogenicity and good biomechanical properties. Methods Pig xenograft bone was degreased with supercritical CO2 and cryopreserved into 3 groups. The antigens were removed by ultrasonic assisted trypsin method (group A). The heterotopic bone was treated by ultrasonic method (group B) or trypsin method (group C). The antigenicity and biomechanical properties of bone materials were observed and compared. Results Ultrasound-assisted enzymatic antigen-removal of xenogeneic bone was faster, and the treated porcine xenograft material had more complete antigenation, lower immunogenicity, the highest porosity and the best pore size, but the biomechanical properties of the treated porcine xenogeneic bone materials were obviously decreased. Conclusion Ultrasound-assisted enzymatic method is a good antigen-removing method for bone materials, but the biomechanical properties of the materials are significantly affected after the treatment, and further optimization of experimental parameters is needed.
【作者單位】： 貴州省人民醫(yī)院骨科;
【基金】：貴州省科學(xué)技術(shù)基金資助項目(黔合科J字[2010]2276號)
【分類號】：R687.3;R318.08
，

本文編號：2428756