【摘要】：根據(jù)在NCBI中已登錄的植物轉(zhuǎn)錄因子NAC家族中與抗逆性有關(guān)成員的核苷酸序列和氨基酸序列,應(yīng)用生物信息學(xué)軟件分析了其理化性質(zhì)、疏水性/親水性、跨膜結(jié)構(gòu)、二級結(jié)構(gòu)、功能結(jié)構(gòu)域等,并構(gòu)建了這類NAC家族成員的系統(tǒng)進(jìn)化樹。結(jié)果表明,17種NAC成員的蛋白質(zhì)一級結(jié)構(gòu)中存在明顯的疏水區(qū)和親水區(qū),都存在明顯的ɑ-螺旋和β折疊。二級結(jié)構(gòu)組成上相似,都由ɑ-螺旋、β折疊和無規(guī)則卷曲組成。這些成員都能夠通過同源建模分析NAC蛋白質(zhì)的三維結(jié)構(gòu)。進(jìn)化分析表明,它們主要分成4大類群。通過多序列比對得到了這些成員的保守區(qū)段,并設(shè)計了簡并引物。
[Abstract]:Based on the nucleotide and amino acid sequences of members of the NAC family of plant transcription factors registered in NCBI, the physicochemical properties, hydrophobicity / hydrophilicity, transmembrane structure and secondary structure were analyzed by bioinformatics software. The functional domain and the phylogenetic tree of this kind of NAC family members are constructed. The results showed that there were obvious hydrophobic and hydrophilic regions in the primary structure of 17 NAC members, and obvious helix and 尾 -folding. The secondary structure is similar in composition, which consists of helix, 尾-fold and irregular crimp. These members were able to analyze the three-dimensional structure of NAC protein by homologous modeling. Evolutionary analysis showed that they were mainly divided into 4 groups. The conservative regions of these members were obtained by multiple sequence alignment and degenerate primers were designed.
【作者單位】： 棗莊學(xué)院生命科學(xué)學(xué)院;
【基金】：棗莊學(xué)院博士科研基金項目(307070910)
【分類號】：R318.04

【共引文獻(xiàn)】

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