基于反離子電滲透原理的無(wú)創(chuàng)血糖檢測(cè)研究
發(fā)布時(shí)間：2018-12-15 04:29
【摘要】：糖尿病是影響人類健康的三大殺手之一，世界上糖尿病患者數(shù)量巨大且越來(lái)越多，目前沒(méi)有根治糖尿病的方法，只有通過(guò)頻繁的血糖濃度檢測(cè)，注射胰島素控制血糖濃度來(lái)預(yù)防和治療糖尿病及其并發(fā)癥。無(wú)創(chuàng)血糖檢測(cè)技術(shù)是血糖檢測(cè)研究的熱點(diǎn)，主要研究方法是近紅外光法和反離子電滲透法。本文探討了利用反離子電滲透原理進(jìn)行組織液葡萄糖提取和滲透出的組織液葡萄糖濃度測(cè)定研究，設(shè)計(jì)了能夠用于葡萄糖檢測(cè)的電流型葡萄糖傳感器，并利用單片機(jī)集成電路技術(shù)，以STC89C51RC為核心，設(shè)計(jì)能夠進(jìn)行初步提取和檢測(cè)葡萄糖濃度的血糖檢測(cè)裝置。 論文主要完成的工作如下： （1）完成了基于絲網(wǎng)印刷工藝的三電極電流型葡萄糖傳感器研制，經(jīng)CHI600A電化學(xué)工作站檢測(cè)，該傳感器具有較好的氧化還原性、線性度、可重復(fù)性及一致性。在葡萄糖溶液濃度為4~22mmol/L時(shí)，傳感器的電流響應(yīng)與葡萄糖濃度間有較好的線性關(guān)系，同一批次和不同批次設(shè)計(jì)的傳感器測(cè)量的平均偏差為0.85%，具有較好的重復(fù)性和一致性。本傳感器初步實(shí)現(xiàn)了mmol/L級(jí)葡萄糖濃度的檢測(cè)。 （2）研制了基于反離子電滲透原理的血糖檢測(cè)組織液提取和檢測(cè)裝置。以STC89C51RC主控芯片為基礎(chǔ)，研制了0.1mA/cm2的恒流源提取電路和5.0~11.0V可調(diào)的恒壓源電路，用于葡萄糖的提取實(shí)驗(yàn)。還研制了弱電流信號(hào)的檢測(cè)和顯示電路用于葡萄糖溶液的顯示。經(jīng)電路調(diào)試發(fā)現(xiàn)，設(shè)計(jì)的恒壓源和恒流源電路達(dá)到所需電流和電壓要求，可用于葡萄糖的提��；實(shí)現(xiàn)了信號(hào)采集和顯示功能，，能用于葡萄糖傳感器上產(chǎn)生弱電流信號(hào)的檢測(cè)和顯示。 （3）最后，利用該裝置對(duì)4~22mmol/L的葡萄糖溶液進(jìn)行測(cè)試，驗(yàn)證所研制的傳感器和無(wú)創(chuàng)血糖檢測(cè)裝置的性能。試驗(yàn)結(jié)果表明，裝置能把不同濃度的葡萄糖溶液很好的區(qū)分，且檢測(cè)結(jié)果與三諾安穩(wěn)血糖儀檢測(cè)結(jié)果的相關(guān)系數(shù)為0.9980。
[Abstract]:Diabetes is one of the three major killers affecting human health. There are large and growing numbers of diabetes patients in the world. There is no cure for diabetes at present, only through frequent blood glucose testing. Insulin injection controls blood glucose levels to prevent and treat diabetes and its complications. Non-invasive blood glucose detection is a hot spot in blood glucose detection. The main research methods are near infrared light and reverse ion electroosmosis. In this paper, using the principle of reverse ion electroosmosis, we have studied the determination of glucose concentration in tissue solution for glucose extraction and osmosis, and designed an amperometric glucose sensor which can be used for glucose detection. Using the integrated circuit technology of single chip computer and STC89C51RC as the core, a blood glucose detection device was designed to carry out preliminary extraction and detection of glucose concentration. The main work of this thesis is as follows: (1) the three-electrode current type glucose sensor based on screen printing technology has been developed and tested by CHI600A electrochemical workstation. The sensor has good redox and linearity. Repeatability and consistency. When the concentration of glucose solution is 4~22mmol/L, there is a good linear relationship between the current response of the sensor and the concentration of glucose. The average deviation of the sensor designed for the same batch and different batches is 0.85. It has good repeatability and consistency. This sensor has preliminarily realized the detection of mmol/L grade glucose concentration. (2) based on the principle of reverse ion electroosmosis, a device for extracting and detecting tissue fluid for blood glucose detection was developed. Based on the STC89C51RC main control chip, a constant current source extraction circuit for 0.1mA/cm2 and an adjustable constant voltage source circuit of 5.0 ~ 11.0V have been developed for glucose extraction experiments. A weak current signal detection and display circuit is also developed for glucose solution display. The circuit debugging shows that the designed constant voltage source and constant current source circuit meet the required current and voltage requirements and can be used to extract glucose. The function of signal acquisition and display is realized, which can be used to detect and display weak current signal generated on glucose sensor. (3) finally, the glucose solution of 4~22mmol/L was tested by the device to verify the performance of the sensor and the non-invasive blood glucose detection device. The experimental results show that the device can distinguish different concentrations of glucose solution well, and the correlation coefficient between the detection results and the results of Sannoan stable blood glucose meter is 0.9980.
【學(xué)位授予單位】：重慶理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R318.6
本文編號(hào)：2379976
[Abstract]:Diabetes is one of the three major killers affecting human health. There are large and growing numbers of diabetes patients in the world. There is no cure for diabetes at present, only through frequent blood glucose testing. Insulin injection controls blood glucose levels to prevent and treat diabetes and its complications. Non-invasive blood glucose detection is a hot spot in blood glucose detection. The main research methods are near infrared light and reverse ion electroosmosis. In this paper, using the principle of reverse ion electroosmosis, we have studied the determination of glucose concentration in tissue solution for glucose extraction and osmosis, and designed an amperometric glucose sensor which can be used for glucose detection. Using the integrated circuit technology of single chip computer and STC89C51RC as the core, a blood glucose detection device was designed to carry out preliminary extraction and detection of glucose concentration. The main work of this thesis is as follows: (1) the three-electrode current type glucose sensor based on screen printing technology has been developed and tested by CHI600A electrochemical workstation. The sensor has good redox and linearity. Repeatability and consistency. When the concentration of glucose solution is 4~22mmol/L, there is a good linear relationship between the current response of the sensor and the concentration of glucose. The average deviation of the sensor designed for the same batch and different batches is 0.85. It has good repeatability and consistency. This sensor has preliminarily realized the detection of mmol/L grade glucose concentration. (2) based on the principle of reverse ion electroosmosis, a device for extracting and detecting tissue fluid for blood glucose detection was developed. Based on the STC89C51RC main control chip, a constant current source extraction circuit for 0.1mA/cm2 and an adjustable constant voltage source circuit of 5.0 ~ 11.0V have been developed for glucose extraction experiments. A weak current signal detection and display circuit is also developed for glucose solution display. The circuit debugging shows that the designed constant voltage source and constant current source circuit meet the required current and voltage requirements and can be used to extract glucose. The function of signal acquisition and display is realized, which can be used to detect and display weak current signal generated on glucose sensor. (3) finally, the glucose solution of 4~22mmol/L was tested by the device to verify the performance of the sensor and the non-invasive blood glucose detection device. The experimental results show that the device can distinguish different concentrations of glucose solution well, and the correlation coefficient between the detection results and the results of Sannoan stable blood glucose meter is 0.9980.
【學(xué)位授予單位】：重慶理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R318.6
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