核素切倫科夫光學(xué)顯像基礎(chǔ)研究
發(fā)布時(shí)間：2018-12-10 22:01
【摘要】：目的 研究不同核素的切倫科夫光學(xué)顯像的光學(xué)信號(hào)在不同組織中的穿透性差異及空間分辨率變化。并且設(shè)計(jì)一種稀土納米顆粒對(duì)切倫科夫光進(jìn)行轉(zhuǎn)換以增強(qiáng)其信號(hào)強(qiáng)度和穿透性。 方法 將活度為0，3，6，12，25，50uCi的131I和18F-FDG分別加入24孔板中，進(jìn)行切倫科夫光學(xué)顯像，進(jìn)行切倫科夫光學(xué)信號(hào)與放射性活度的相關(guān)性分析。制作放射性濃度為20，50，100，200μCi/μl，長(zhǎng)度為100mm，，直徑為1mm的線狀131I放射源，間隔1cm平行放置，分別覆蓋不同厚度的脂肪、肝、肺和肌肉組織片，進(jìn)行光學(xué)顯像；觀察不同活度的131I對(duì)不同組織的光信號(hào)穿透性差異。制作放射性濃度為100uCi/μL，長(zhǎng)度為100mm，直徑為1mm的線狀131I和18F放射源，間隔2cm平行放置；上方覆蓋不同厚度的脂肪、肝、肺和肌肉組織薄片進(jìn)行切倫科夫光學(xué)顯像，研究切倫科夫光學(xué)顯像空間分辨率的變化。然后利用水熱法合成Yb3+-和Er3+-摻雜的NaYF4稀土納米顆粒。利用掃描電鏡和X射線衍射對(duì)其進(jìn)行表征并利用光譜儀測(cè)定其吸收和發(fā)射光譜。將其溶于水中加入發(fā)射性核素，利用光學(xué)顯像測(cè)定其對(duì)切倫科夫光信號(hào)的增強(qiáng)作用，同時(shí)利用仿體模型測(cè)定加入稀土納米顆粒后核素切倫科夫光的穿透性變化。最紅利用荷假瘤小鼠進(jìn)行正電子發(fā)射斷層顯像和光學(xué)顯像驗(yàn)證這一增強(qiáng)效果。 結(jié)果 切倫科夫光信號(hào)強(qiáng)度隨放射性活度的增加呈線性增加(r2=0.97，P=0.0001和r2=0.96，P=0.0001)。同時(shí)切倫科夫光信號(hào)的強(qiáng)度隨光源的深度增加而下降。其中以脂肪組織的光透過性最好。另外，切倫科夫光學(xué)顯像的空間分辨率隨放射性活度的降低和組織深度的增加而下降；同時(shí)對(duì)不同組織的空間分辨率各異，其中以脂肪組織中成像的空間分辨率最好。之后的增強(qiáng)實(shí)驗(yàn)顯示切倫科夫輻射可以在520nm和980nm波段激發(fā)稀土納米顆粒在660nm波段發(fā)光。加入稀土納米顆粒后，切倫科夫光強(qiáng)度提升約2倍而組織穿透性從5mm明顯提高至15mm。并且信號(hào)增強(qiáng)的強(qiáng)度與放射性核素活度(R2=0.996)及稀土納米顆粒的質(zhì)量(R2=0.994)有關(guān)。動(dòng)物實(shí)驗(yàn)則驗(yàn)證了這一效應(yīng)的可行性。 結(jié)論 切倫科夫光學(xué)顯像的光穿透性及空間分辨率受核素類型、放射性活度、組織類型及組織深度等因素影響較為明顯。其光信號(hào)穿透性差，而分辨率較高。在加入稀土納米顆粒后，切倫科夫光信號(hào)強(qiáng)度和穿透性都明顯提升，為進(jìn)一步的應(yīng)用奠定了基礎(chǔ)。
[Abstract]:Aim to study the difference of optical signal penetration and spatial resolution in different tissues of Cerenkov optical imaging with different nuclides. A rare earth nanoparticles were designed to convert the Cerenkov light to enhance its signal intensity and penetration. Methods 131I and 18F-FDG were added to the 24-hole plate respectively, and the correlation between the Cerenkov optical signal and the radioactivity was analyzed. A linear 131I radioactive source with a diameter of 100mm and a diameter of 1mm 渭 Ci/ 渭 l was prepared. The spacer 1cm was placed parallel to each other and covered with different thickness of fat, liver, lung and muscle tissue, respectively, for optical imaging. The optical signal penetration of 131I with different activity to different tissues was observed. 100uCi/ 渭 L, 100mm long, 131I and 18F radioactive sources with diameter of 1mm were prepared, and the 2cm was spaced parallel to each other. The different thickness of fat, liver, lung and muscle tissue were covered with Cerenkov optical imaging to study the spatial resolution of Cerenkov optical imaging. Then Yb3-and Er3-doped NaYF4 rare earth nanoparticles were synthesized by hydrothermal method. The absorption and emission spectra were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD). The enhancement of Cerenkov light signal was measured by optical imaging, and the penetrability of Cerenkov light with rare earth nanoparticles was measured by the imitating body model. Positron emission tomography (PET) and optical imaging were performed in mice bearing pseudotumor. Results the intensity of Cerenkov light signal increased linearly with the increase of radioactivity (r _ (2) O ~ (97) P ~ (0. 0001) and r ~ (2 +) ~ (0.96) P ~ (2 +) P ~ (0.0001). At the same time, the intensity of the Cherenkov light signal decreases with the increase of the depth of the light source. Among them, adipose tissue has the best light transmittance. In addition, the spatial resolution of Cerenkov optical imaging decreases with the decrease of radioactivity and the increase of tissue depth, and the spatial resolution of different tissues is different, and the spatial resolution of imaging in adipose tissue is the best. The subsequent enhancement experiments show that Cerenkov radiation can excite rare earth nanoparticles in the 520nm and 980nm bands to emit light in the 660nm band. With the addition of rare earth nanoparticles, the light intensity of Cerenkov was increased by about 2 times, while the penetration of the tissue was increased from 5mm to 15mm. The intensity of signal enhancement is related to the activity of radionuclide (R2N) 0.996 and the mass of rare earth nanoparticles (R2O0.994). Animal experiments verify the feasibility of this effect. Conclusion the optical penetrability and spatial resolution of Cerenkov optical imaging are obviously influenced by the type of radionuclide, the activity of radionuclide, the type of tissue and the depth of tissue. The optical signal has poor penetration and high resolution. After the addition of rare earth nanoparticles, the intensity and penetration of Cerenkov light signal were improved obviously, which laid a foundation for further application.
【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R310
[Abstract]:Aim to study the difference of optical signal penetration and spatial resolution in different tissues of Cerenkov optical imaging with different nuclides. A rare earth nanoparticles were designed to convert the Cerenkov light to enhance its signal intensity and penetration. Methods 131I and 18F-FDG were added to the 24-hole plate respectively, and the correlation between the Cerenkov optical signal and the radioactivity was analyzed. A linear 131I radioactive source with a diameter of 100mm and a diameter of 1mm 渭 Ci/ 渭 l was prepared. The spacer 1cm was placed parallel to each other and covered with different thickness of fat, liver, lung and muscle tissue, respectively, for optical imaging. The optical signal penetration of 131I with different activity to different tissues was observed. 100uCi/ 渭 L, 100mm long, 131I and 18F radioactive sources with diameter of 1mm were prepared, and the 2cm was spaced parallel to each other. The different thickness of fat, liver, lung and muscle tissue were covered with Cerenkov optical imaging to study the spatial resolution of Cerenkov optical imaging. Then Yb3-and Er3-doped NaYF4 rare earth nanoparticles were synthesized by hydrothermal method. The absorption and emission spectra were characterized by scanning electron microscope (SEM) and X-ray diffraction (XRD). The enhancement of Cerenkov light signal was measured by optical imaging, and the penetrability of Cerenkov light with rare earth nanoparticles was measured by the imitating body model. Positron emission tomography (PET) and optical imaging were performed in mice bearing pseudotumor. Results the intensity of Cerenkov light signal increased linearly with the increase of radioactivity (r _ (2) O ~ (97) P ~ (0. 0001) and r ~ (2 +) ~ (0.96) P ~ (2 +) P ~ (0.0001). At the same time, the intensity of the Cherenkov light signal decreases with the increase of the depth of the light source. Among them, adipose tissue has the best light transmittance. In addition, the spatial resolution of Cerenkov optical imaging decreases with the decrease of radioactivity and the increase of tissue depth, and the spatial resolution of different tissues is different, and the spatial resolution of imaging in adipose tissue is the best. The subsequent enhancement experiments show that Cerenkov radiation can excite rare earth nanoparticles in the 520nm and 980nm bands to emit light in the 660nm band. With the addition of rare earth nanoparticles, the light intensity of Cerenkov was increased by about 2 times, while the penetration of the tissue was increased from 5mm to 15mm. The intensity of signal enhancement is related to the activity of radionuclide (R2N) 0.996 and the mass of rare earth nanoparticles (R2O0.994). Animal experiments verify the feasibility of this effect. Conclusion the optical penetrability and spatial resolution of Cerenkov optical imaging are obviously influenced by the type of radionuclide, the activity of radionuclide, the type of tissue and the depth of tissue. The optical signal has poor penetration and high resolution. After the addition of rare earth nanoparticles, the intensity and penetration of Cerenkov light signal were improved obviously, which laid a foundation for further application.
【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R310
【共引文獻(xiàn)】
相關(guān)期刊論文 前10條
1 康飛;楊衛(wèi)東;汪靜;;對(duì)切倫科夫光學(xué)成像應(yīng)用前景的思考[J];醫(yī)學(xué)爭(zhēng)鳴;2013年06期
2 張慶彬;孔祥貴;王新;程成;;NaYF_4:Yb~(3+),Er~(3+)上轉(zhuǎn)換發(fā)光納米晶在三元混合溶劑體系中的配體交換修飾及發(fā)光性質(zhì)[J];高等學(xué)�；瘜W(xué)學(xué)報(bào);2014年02期
3 呂光磊;劉克印;孟路燕;易濤;;二芳乙烯熒光開關(guān)材料及其應(yīng)用進(jìn)展[J];影像科學(xué)與光化學(xué);2014年01期
4 楊衛(wèi)東;汪靜;;核素切倫科夫顯像研究進(jìn)展[J];功能與分子醫(yī)學(xué)影像學(xué)(電子版);2013年03期
5 李向麗;譚貴良;張娜;劉W
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