【摘要】：目的軟骨細(xì)胞復(fù)合同種異體脫鈣松質(zhì)骨(demineralized cancellous bone,DCB)體外構(gòu)建組織工程軟骨,評價DCB作為組織工程軟骨支架的可行性。方法取新西蘭兔長骨干骺端松質(zhì)骨制備DCB,掃描電鏡觀察;分離幼兔關(guān)節(jié)軟骨細(xì)胞,原代培養(yǎng)后種植在同種異體DCB體外培養(yǎng),分別于7、14、21、28、35、42d取材進(jìn)行蘇木素-伊紅(HE)、甲苯胺藍(lán)(TB)、Ⅰ型和Ⅱ型膠原染色。結(jié)果制備的DCB成三維立體多孔結(jié)構(gòu),孔隙大小100~500μm,有良好的可塑性和一定的力學(xué)強(qiáng)度;軟骨細(xì)胞在DCB表面和空隙內(nèi)生長、分化良好,形成軟骨陷窩,分泌基質(zhì)蛋白聚糖和Ⅱ型膠原。培養(yǎng)42d軟骨細(xì)胞在DCB表面及孔隙內(nèi)形成軟骨樣組織。結(jié)論 DCB復(fù)合同種異體軟骨細(xì)胞在體外成功構(gòu)建了組織工程軟骨,是一種較理想的軟骨組織工程支架材料。
[Abstract]:Objective to construct tissue engineered cartilage with chondrocytes combined with allogeneic decalcified cancellous bone (demineralized cancellous bone,DCB) in vitro and evaluate the feasibility of DCB as scaffold for tissue engineering cartilage. Methods New Zealand rabbit epiphyseal cancellous bone was prepared by DCB, scanning electron microscope. Articular chondrocytes were isolated from young rabbits and cultured in vitro with allogeneic DCB. The specimens were collected for (TB), 鈪，

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