【摘要】：目的采用抗菌肽(GBB)替代傳統(tǒng)抗菌劑,制備新型濕性抑菌水凝膠敷料。方法將聚丙烯酸鈉固定于無紡布上作為抑菌敷料的基底,并用涂抹方式在基底表面吸附上0.004 mg/cm2~1.00 mg/cm2不同濃度的抗菌肽。參照標(biāo)準(zhǔn)YY/T0471.1-2004中的方法測(cè)定敷料的吸水率、含水率及水蒸氣透過率;采用2015版《中國(guó)藥典》通則0952黏附力測(cè)定第一法,測(cè)定初粘力表征材料理化性能;同時(shí)采用接觸法及抑菌圈法檢測(cè)敷料抑菌性,評(píng)價(jià)抑菌敷料抑菌作用大小,并與商業(yè)化含銀敷料抑菌率進(jìn)行比較;用噻唑藍(lán)(MTT)法評(píng)估檢測(cè)材料有無明顯細(xì)胞毒性。結(jié)果敷料吸水率平均值可達(dá)404.59%,含水率平均值可達(dá)80.09%,水蒸氣透過率平均值可達(dá)1476.67g/m~2/24h,能黏附最大鋼球質(zhì)量10.10g,敷料表面GBB含0.20 mg/cm~2,對(duì)大腸埃希菌抑菌率為100%,MTT法檢測(cè)材料無明顯毒性。結(jié)論材料基本滿足濕性抑菌水凝膠敷料要求,具有優(yōu)良生物相容性,故有望作為抗感染的創(chuàng)面敷料。
[Abstract]:Objective to prepare a new hydrogel dressing with antimicrobial peptide (GBB) instead of traditional antimicrobial agent. Methods Sodium polyacrylate was immobilized on the non-woven fabric as the substrate of bacteriostasis dressing, and 0.004 mg/cm2~1.00 mg/cm2 antimicrobial peptides were adsorbed on the substrate surface by smear method. The water absorption, moisture content and water vapor transmittance of the dressing were determined by referring to the method in standard YY/T0471.1-2004, the first method was used to determine the adhesion force of the dressing under the general rule 0952 of Chinese Pharmacopoeia, and the physical and chemical properties of the material were characterized by the determination of the initial adhesion. At the same time, contact method and bacteriostatic circle method were used to test the bacteriostasis of the dressing, to evaluate the bacteriostatic effect of the antifungal dressing, and to compare the bacteriostatic rate of the antibacterial dressing with commercial silver dressing. The thiazolyl (MTT) method was used to evaluate the cytotoxicity of the material. Results the average water absorption rate of dressing was 404.59, the average water content was 80.09, the average water vapor transmittance was 1476.67 g / m ~ 2 / 24 h, and the maximum steel ball mass could be adhered to 10.10 g. The bacteriostatic rate of GBB on dressing surface was 0.20 mg/cm~2, and the bacteriostatic rate of Escherichia coli was 100%. Conclusion the material can meet the requirements of wettable bacteriostasis hydrogel dressing and has good biocompatibility, so it is expected to be used as an anti-infection wound dressing.
【作者單位】： 浙江省醫(yī)療器械檢驗(yàn)院;杭州先端生物技術(shù)有限公司;
【基金】：浙江省省屬科研院所基金資助項(xiàng)目(2017F50029)
【分類號(hào)】：R318.08
，

本文編號(hào)：2258050