【摘要】：背景:納米羥基磷灰石/聚酰胺66是一種新型的納米仿生復(fù)合材料,具有良好的組織相容性以及骨傳導(dǎo)性,但其植入體內(nèi)后和骨界面的接合生長(zhǎng)如何、臨床應(yīng)用是否安全等尚需要進(jìn)一步研究。目的:觀察納米羥基磷灰石/聚酰胺復(fù)合材料在人工肱骨頭柄界面的結(jié)合能力。方法:將兔隨機(jī)分為2組,均進(jìn)行肩關(guān)節(jié)肱骨頭轉(zhuǎn)換,復(fù)合材料組植入納米羥基磷灰石/聚酰胺人工肱骨頭;對(duì)照組植入自體髂骨。置換后對(duì)兔進(jìn)行骨髓間充質(zhì)干細(xì)胞原代培養(yǎng),再將骨髓間充質(zhì)干細(xì)胞與納米羥基磷灰石/聚酰胺復(fù)合材料共培養(yǎng)進(jìn)行觀察。結(jié)果與結(jié)論:細(xì)胞培養(yǎng)24 h后,骨髓間充質(zhì)干細(xì)胞在復(fù)合材料局部生長(zhǎng)情況較好。復(fù)合材料組培養(yǎng)1,4 h的細(xì)胞黏附率顯著高于對(duì)照組(P0.05)。復(fù)合材料組共培養(yǎng)4.5,5.5,6.5 h細(xì)胞吸光度值百分率值顯著高于對(duì)照組(P0.05);置換24周后復(fù)合材料組表面骨組織、纖維層的比例變化幅度及覆蓋率最高(P0.05)。結(jié)果證實(shí),納米羥基磷灰石/聚酰胺復(fù)合材料植入體柄具有一定的界面骨結(jié)合能力,可使界面骨整合良好。
[Abstract]:Background: Nano-hydroxyapatite / polyamide 66 is a novel nano-biomimetic composite with good histocompatibility and bone conductivity. Whether clinical application is safe or not needs further study. Aim: to observe the binding ability of nano-hydroxyapatite / polyamide composite at the interface of humerus handle. Methods: rabbits were randomly divided into two groups. The humerus head of shoulder joint was transformed into composite group with nano-hydroxyapatite / polyamide artificial humerus and the control group with autogenous iliac bone. Bone marrow mesenchymal stem cells (BMSCs) were cultured in primary culture and co-cultured with nano-hydroxyapatite / polyamide composite material after replacement of rabbit bone marrow mesenchymal stem cells (BMSCs). Results and conclusion: bone marrow mesenchymal stem cells grew well in composite materials after 24 hours of cell culture. The cell adhesion rate of the composite group was significantly higher than that of the control group for 4 h (P0.05). The percentage of cell absorbance in the composite group was significantly higher than that in the control group (P0.05), and the ratio of the fibrous layer were the highest in the composite group after 24 weeks of replacement (P0.05). The results showed that nano-hydroxyapatite / polyamide composite implants had some interfacial bone binding ability, which could make the interfacial bone integrate well.
【作者單位】： 宜賓市第一人民醫(yī)院骨一科;重慶醫(yī)科大學(xué)附屬第一醫(yī)院骨科;
【分類號(hào)】：R318.08

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本文編號(hào)：2225646